Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"vesicle formation"”.
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Theoretical and experimental studies of vesicle formation in surfactant mixtures
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1996.
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Proteins that interact with Sec16p during COPII vesicle formation in Saccharomyces cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Regulation of COPII vesicle formation and protein sorting by the BST genes in Saccharomyces cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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An investigation of the functional role of GRAB in the context of Rab3, Rab8 and Rab11
… regulate membrane trafficking processes, such as vesicle formation, vesicle trafficking within the cell and fusion with an acceptor compartment. Multiple effector proteins have been identified for many Rabs, some of which can interact with more than one Rab to link their function at a specific …
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The behaviour of fatty acid vesicles in prebiotically plausible environments
… of model protocells assembled from fatty acid vesicles is lacking in comparison to the abundant literature on phospholipid vesicles. There is evidence that the existence of fatty acids predated life on Earth. Moreover, the highly dynamic nature of fatty acid vesicles is not well documented in …
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Overcoming the plasma membrane barrier to improve the efficiency of therapeutic delivery to the cellular cytoplasm
… of targeted Connectosomes, cell-derived lipid vesicle materials that contain embedded connexons and are capable of forming functional gap junctions with cells. These materials encapsulated diverse molecular cargo, including dyes and drugs. These materials achieved efficient delivery of …
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Analysis of the recruitment of the class I myosin Myo5p to endocytic sites in Saccharomyces cerevisiae
… as sites of endocytosis. During endocytic vesicle formation, proteins associate and dissociate at these membrane subdomains in a highly defined temporal order. The type I myosin Myo5p is an essential component of the endocytic machinery of S. cerevisiae. The protein has been shown to be …
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Characterization of Alsin and Its Role in IGF-1-Mediated Neuronal Survival
… involved in each trafficking step with roles in vesicle formation, budding, movement, and fusion. One class of proteins, the Rab GTPases, is required for docking and fusion of transport vesicles with their target membrane. These proteins are regulated by their state of nucleotide binding, with …
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Regulatory domains of the Arf GEF Sec7 scaffold autoinhibited and active conformations
… dependent on the small GTPase Arf1 which drives vesicle formation and membrane modification following its activation by the Arf GEF Sec7. A network of inter- and intramolecular interactions of Sec7 gives robust spatiotemporal specificity to the potent Arf GEF. The Sec7 homodimer is autoinhibited, …
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Novel pleiotropic regulators of gas vesicle biogenesis in Serratia
… production. In addition, S39006 produces gas vesicles to float in aqueous environments and enable colonization of air-liquid interfaces. Gas vesicles are thought to be constructed solely from proteins expressed from a gene cluster composed of two contiguous operons, gvpA1-gvpY and gvrA-gvrC. …
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The effect of hyperosmolarity on fluid-phase and receptor-mediated endocytosis in P388D1 macrophages
… of receptor-ligand complexes into coated vesicles of about 0.1 μm in diameter. The average diameter of primary pinocytic vesicles has been calculated to be 0.24 - 0.28 μm. The discrepancy in size between coated vesicles and the average pinosome diameter can be explained if, in addition to …
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Lipid rafts in protein sorting and yeast cell polarity
… characterize the lipid composition of secretory vesicles. Hence, we established a vesicle purification protocol based on an immunoisolation strategy. Additionally, in collaboration with the group of A. Shevchenko, we developed a mass spectrometry methodology that allows the comprehensive and …
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THE OPTIMIZATION OF A TIME-RESOLVED, VESICLE-BASED FLUORESCENCE ASSAY FOR THE ACTIVITY OF THE LIPID KINASE PI4K-IIIBETA AND THE EFFECT OF LIPID TRANSFER PROTEINS ON ENZYME ACTIVITY
… network where it is essential for secretory vesicle formation, as well as the precursor for other phosphoinositides that are crucial for intracellular signalling. Among others, phosphoinositide homeostasis in eukaryotic membranes rely on PI kinases and PI transfer proteins (PITPs). In yeast, …
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Principles of COPII Coat Assembly
… membrane and allow budding of the cargo-loaded vesicle. My work provides evidence that the essential connection between the two coat layers is established by multiple different interfaces between Sec23 and the Sec31 disordered proline-rich domain. The Sec31 unstructured domain comprises known …
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Roles for cell surface glycans in guiding human pluripotent stem cell fate
… distribution and ultrastructure of this information-rich, signaling polysaccharides are poorly defined. In this work, I aim to understand the interplay between the HS organization and the developmental signal transduction in the hPS cells’ microenvironment. We discovered that HS of hPS …
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Interfacial Phenomena and Surface Forces of Hydrophobic Solids
… self-assembly in biological processes, such as vesicle formation and protein folding. Although it is known that a strong attraction between apolar molecules exists at the molecular level, it is not clear how this force scales up to objects with dimensions in the range 100 nm–1 m. This work sets …
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Investigation of stability, dynamics and scope of application of mycobacterial porin MspA: a highly versatile biomolecular resource
… scattering and X-ray crystallography. Nanoscopic vesicle formation of MspA molecules in aqueous media has been thoughroughly investigated. Temperature dependent dynamic light scattering experiments reveal that size of such vesicles is dependent on temperature but is independent of ionic strength …
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Translational tuning pre-emptively modulates protein folding and secretory pathway defects
… coat protein that generates ER-derived transport vesicles. Loss of Eap1 and other translation regulators exacerbates various Sec24 growth phenotypes. I further show that translation at the ER is repressed in cells where COPII vesicle formation is impaired by Sec24 mutation and propose that this …
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Effects of Light on BDBT Puncta Formation in the Drosophila Eye Reveal Involvement of BDBT with Color Preference Behavior and Distinct Nuclear Localization Pathways for DBT and PER
… behind BRIDE OF DOUBLETIME (BDBT) puncta/foci formation. During this process it was discovered that both the circadian and visual photoreceptors have roles for normal cycles of BDBT foci formation. This BDBT foci cycling is needed for normal changes in subcellular localization of the circadian …
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Conserved and Novel Properties of Clathrin-Mediated Endocytosis in Dictyostelium Discoideum
… is the adaptor complex AP-2, to co-ordinate vesicle formation. Disruption of genes involved in clathrin-mediated endocytosis causes embryonic lethality in multicellular animals suggesting that clathrin-mediated endocytosis is a fundamental cellular process. However, loss of clathrin-mediated …
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