Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"variant surface glycoprotein (VSG)"”.
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Discovery and development of diagnostic biomarkers for human African trypanosomiasis
… patients’ sera from the WHO HAT specimen bank. Invariant Surface Glycoprotein (ISG) 65 and a soluble Variant Surface Glycoprotein (VSG) were selected for development into a lateral flow format and 80 randomised patients’ sera were used to evaluate these prototypes. Here we describe the results …
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Antigenic Variation and Telomere Structure in Trypanosoma brucei
… its mammalian hosts by periodically changing the variant surface glycoprotein (VSG). From the ~1000 VSG genes (VSGs) scattered throughout the genome, a few are located at telomere loci called expression sites (ESs) and only one is expressed at any time, suggesting a stringent regulatory mechanism. …
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Characterisation of potential Holliday junction resolvases in Trypanosoma brucei
Antigenic variation on the cell surface of the T. brucei is essential for the survival of the parasite in the host immune system. Finding mechanisms to stop variant surface glycoprotein (VSG) switching, and hence the parasites ability to evade the host immune system provides a promising opportunity …
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VSG expression in African trypanosomes: Gene silencing, cell cycle and developmental control
… trypanosomes undergo antigenic variation of the variant surface glycoprotein (VSG) coat. The trypanosome genome contains ~2,000 VSG genes, however only a single VSG gene is transcribed with several expression site associated genes (ESAGs) in a polycistronic transcription unit by RNA-polymerase I, …
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Tracking the In Vivo Dynamics of Antigenic Variation in the African Trypanosome
… nagana in animals, constantly changes its dense variant surface glycoprotein (VSG) coat to avoid elimination by the immune system of its mammalian host, using an extensive repertoire of dedicated genes. Although this process, referred to as antigenic variation, is the major mechanism of …
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Analysis of the functions and interactions of RAD51 paralogues in Trypanosoma brucei
… variation, involving periodic switches in the variant surface glycoprotein (VSG) coat. DNA recombination is critical in this process and a key component of homologous recombination, RAD51, also plays a role in VSG switching. T. brucei encodes four proteins distantly related to RAD51; termed …
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Structure of the trypanosome transferrin receptor and insights into ligand binding and therapeutic strategies
… and protect against the host immune system. The variant surface glycoprotein (VSG) forms a dense and dynamic protective coat, which serves to defend against the immunoglobulin-mediated host response. In addition, a unique set of receptors mediate uptake of host macromolecules and must comply with …
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Macrophage cytokines as correlate of differential resistance to African trypanosomiasis
… of 'T. congolense' mediated by antibodies to variant surface glycoprotein (VSG) of IgG2a isotype induced several fold higher NO than phagocytosis mediated by IgM antibodies. NO had a trypanostatic effect on 'T. congolense in vitro' but an influence on the infectivity of the parasites 'in vivo' …
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Surface Coat Replacement Dynamics and Antigen Glycosylation in T. Brucei Influence Evasion of the Host Antibody Response
… host immunity through antigenic variation of its variant surface glycoprotein (VSG) coat. During infection, the VSG coat elicits a robust antibody response, but the parasite escapes antibody-mediated clearance by repeatedly accessing its large genomic VSG repertoire and switching expression to …
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Expression and behaviour of GPI-anchored surface proteins in trypanosomes
… African trypanosome species have evolved a variant surface glycoprotein (VSG) coat which underpins a population survival strategy based on antigenic variation. However, the vast majority of trypanosome species do not use this strategy and our understanding of the immune evasion strategies …
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Characterisation of factors that regulate homologous recombination and antigenic variation in Trypanosoma brucei
… variation, which involves switches in the Variant Surface Glycoprotein (VSG) expressed on the cell surface. Such reactions can occur at very high rates (~10-3 switches/cell/generation) and occur primarily by the recombination of VSG genes, selected from an enormous silent archive, into …