Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"vaginal epithelial"”.
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Methylation-Specific Differentiation of Vaginal Epithelial Cells for Forensic Tissue Typing by Bisulfite Conversion and Pyrosequencing
… a clear absence of reliable testing to identify vaginal epithelial tissue. Though there are serological tests available for this purpose, tissue-specific methylation markers have recently been investigated as a candidate for the identification of blood, saliva, and spermatozoa.</p> <p>In this …
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Interactions of human immunodeficiency virus type 1 with mucosal epithelial surfaces and Candida albicans
… to be less susceptible to HIV-1 infection than vaginal epithelium. In addition, infections with co-pathogens that elicit immune activation, such as Candida albicans, may also promote HIV-1 infection. The project objectives are to determine whether (i) HIV-1 is able to bind and integrate into …
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Effect of a novel contraceptive compound on vaginal health
… spermicides can cause damage to the vaginal epithelium and can increase susceptibility to sexually transmitted diseases (STDs). As such, our laboratory has identified a novel non-hormonal contraceptive method to block the semen liquefaction process using a small molecule inhibitor …
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Tissue-Specific Mediation of Estrogen-Induced Epithelial Events by the Estrogen Receptor in Female Reproductive Tract Organs
… (ERalpha), which mediates the normal uterine and vaginal epithelial proliferative and differentiative effects of 17-beta estradiol (E2), is present in both stroma and epithelium of female reproductive tract organs. Although, the epithelial response to E2 has been assumed to be mediated directly …
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Exposure of 3D organotypic vaginal tissues to semen induces expression of the chemokine MCP-1, but does not alter tissue integrity
… that human semen induces inflammatory changes in vaginal epithelial cell cultures, which may promote HIV transmission. Since the normal human vagina is differentiated into multiple cell layers, we conducted a study to determine the effect of semen on EpiVaginal™, a 3-D organotypic differentiated …
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DNA Methylation Analysis for Tissue and Age Determination for Forensic Human Identification
… to differentiate sperm from blood, saliva, and vaginal epithelial cells, and age specific markers to estimate the age group of a donor using semen samples. Samples were collected of the four relevant tissue types and the genomic DNA was extracted, quantitated, and bisulfite converted. The …
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High Resolution Melt Analysis of Samples with Differential DNA Methylation to Identify Tissue Source of Origin
… higher than the other tissue types tested (skin epithelial, vaginal epithelial, blood, and buccal), and DDX4 had a melting temperature that was 3.0 lower than the other tissue types. In addition, both of the blood markers, cg-06379435 and cg-08792630 were found to have melting temperatures …
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An attempt to advance seasonal reproductive activity in stoats (Mustela erminea) by use of an artifical photoperiod: A dissertation submitted in partial fulfilment of the requirements of the Degree of Bachelor of Science with Honours at Lincoln University
… samples were collected for hormone analysis. Vaginal cytology and physical changes associated with oestrus were monitored in females and scrotal size was monitored in males. In early spring months there was a high incidence of keratinised vaginal epithelial cells and signs of oestrus in …
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The recombinant expression and localization of TvCP2 of trichomonas vaginalis
… cysteine proteases that have been implicated in vaginal epithelial apoptosis as well as immune system evasion. In the past the various cysteine proteases have been studied as a group, and the following work examines, one specific protease, TvCP2, in detail through Western blot analysis, …
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Characterisation of the HIV inhibitory activity of vaginal lactobacilli isolates from young South African women at high risk of HIV acquisition
… and activating HIV target cells, reducing epithelial barrier function and directly promoting HIV replication. Lactobacillus species on the other hand are thought to protect against HIV by competitive exclusion, producing virucidal hydrogen peroxide (H2O2), maintaining an acidic pH by …
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Identification of Saliva Using DNA Methylation Analysis for Forensic Use
… biological fluids: blood, semen, vaginal epithelial tissue, and saliva. While serological testing can identify these tissue types to some degree of accuracy, there has recently been momentum in research to use DNA methylation for tissue identification.</p> <p>In the current study, …
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Evaluation of immunomodulatory compounds for identification of an anti-herpetic innate immune response profile
… than men, most anti-herpetic strategies focus on vaginal application. One promising intervention involves the use of immunomodulatory compounds to engender an HSV-2 resistant environment by stimulating the natural innate immune defenses of the vaginal mucosa. The compounds consist of …
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Development of Advanced Delivery Systems for Microbicides
… research project is to design and develop a vaginal delivery system for combination of microbicides that can sustain the acidic environment of the vaginal cavity and able to release the loaded microbicides as response to change in pH. Microbicide loaded nanoparticles were prepared from …
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Estrogenic Activity of the Polybrominated Diphenyl Ether Flame Retardant Mixture DE-71
… administered alone had no effect on uterine or vaginal tissues but when administered subcutaneously potentiated estradiol’s effect on uterine weight in a dose-dependent manner. DE-71 administered SQ to BALB/c mice for 34 days slightly increased uterine epithelial height (UEH), vaginal epithelial …
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Gene expression patterns of the female genital tract and immunomodulation by Lactobacillus species
… to investigate interactions between different vaginal Lactobacillus species and the host to elucidate its immunomodulatory mechanisms. Cervical cytobrushes and blood samples were collected from chronically HIV-infected South African women. Cervical and peripheral blood mononuclear cells (CMCs …
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Development and Evaluation Of HIV gp120 Responsive Microbicide Formulation for the Prevention of HIV Sexual Transmission
… there is an urgent need to develop topical (vaginal/rectal) microbicide formulations capable of preventing HIV sexual transmission. The objective of this dissertation is to develop a mannose specific, lectin-based topical microbicide formulation capable of targeting HIV gp120 for the …