Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 91 for “"uridine"”.
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Purification of uridine diphosphate glucuronyltransferase
… of the toxicant, enhancing its elimination. Uridine diphosphate glucuronyl transferase (UDPGT) is one microsomal enzyme involved in phase II reactions. It catalyzes the conjugation of toxic compounds with glucuronic acid in reactions in which uridine diphosphate glucuronic acid (UDPGA) is the …
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"Substitution of ""universal"" Uridine-33 in yeast phenylalanine transfer ribonucleic (Rna)"
Embargo set by: Seth Robbins for item 70706 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs
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Investigations into the mode of action of the DNA uridine endonuclease Mth212 of Methanothermobacter thermautotrophicus ΔH
Die spontane hydrolytische Desaminierung von Cytosin zu Uracil ist eine der häufigsten DNA-Modifikationen während des zellulären Stoffwechsels. Um dieser mutagenen Wirkung zu widerstehen, entfernen die meisten Organismen Uracil-Reste aus der DNA mittels Basen-Exzisionsreparatur (BER). Diese wird …
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Studies into the structural basis of the DNA uridine endonuclease activity of exonuclease III homolog Mth212
… entwickelt, um auf ExoA mit erworbener DNA-Uridinendonukleaseaktivität aus der Bibliothek zu selektieren. Insgesamt wurden drei Selektionsansätze, die jeweils auf B.subtilis- oder E.coli-Genetik basierten, entwickelt und getestet. Im ersten Ansatz sollte eine lytische Mutante des …
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A Study of the Pyrimidine Biosynthesis Pathway and its Regulation in Two Distinct Organisms: Methanococcus jannaschii and Pseudomonas aeruginosa
… (pyrG) mutants. By incorporating a functional uridine (cytidine) kinase gene from E. coli it has been possible to isolate a pyrG mutant. In this novel mutant we have been able to independently manipulate the nucleotide pools and study its effects on the enzymes in the biosynthetic pathway. The …
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Nucleic acid changes during lymphocyte transformation in vitro
… measurements of the incorporation of [5-$^{3}$H] uridine during transformation are described. Such measurements produced results which were difficult to interpret. Most of the chapter is devoted to finding to what extent the incorporation of [5-$^{3}$H] uridine can be treated in simple terms. A …
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Cell cycle control of aspartate transcarbamylase levels in Chlorella sorokiniana
… Chlorella sorokiniana was stabilized in vitro by uridine and UMP with 0.04 mM uridine and 0.05 mM UMP giving half maximal stability. Positive cooperative effects on stabilization were observed for UMP but not uridine. The enzyme was stabilized at all temperatures between 2° and 50°, but in the …
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Investigation of Substrate Specificity in Phosphate Binding Barrels and Mechanistic Studies of Orotidine Monophosphate Decarboxylase
… of orotidine 5'-monophosphate (OMP) and uridine 5'-monophosphate (UMP) with an enormous rate acceleration, 1.8 x 1016 (kcat = 5 s-1, 25°C; knon = 2.8 x 1016 s-1), and proficiency, 7.1 x 1021 M-1 (kcat /KM = 2.0 x 106 M-1 s -1, 25°C), without employing metal ions or other …
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Structure-Function Studies on Aspartate Transcarbamoylase and Regulation of Pyrimidine Biosynthesis by a Positive Activator Protein, PyrR in Pseudomonas putida
… salvage enzymes including nucleoside kinases, uridine phosphorylase and cytidine deaminase. Without a functional nucleoside kinase, it was impossible to feed exogenous uridine to P. putida. To obviate this problem, uridine kinase was transferred to P. putida from E. coli and shown to function …
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Effect of Ozone and Ultraviolet Irradiation Treatments on Listeria monocytogenes Populations in Chill Brines
… residual ozone, studying the effect of ozone on uridine absorbance and determining presence of residual listericidal activity post ozonation. An ozone diffuser was designed using 3/16 inch PVC tubing for the ozonation of brines. The sparger was designed to facilitate better diffusion and its …
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Aspects of purine and pyrimidine metabolism
… number of anions and the lack of competition by uridine is indicative of transport by a general anion transporter, with the ability for concentrative uptake in the absence of other external anions being compatible with transport via a ping-pong mechanism. Inhibition of transport by the specific …
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Nucleoside and oligonucleotide approaches towards potential HIV chemotherapies
… a series of nucleoside analogues derived from uridine, 2'-deoxyuridine and AZT. Crystal structures of six compounds were successfully determined. Anti-HIV activity was observed for most compounds in the series although none were without cytotoxicity. The most active compound of the series was …
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Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes.
… of intact cells in the presence of [³H]-uridine and actinomycin D was shown to be very similar with respect to S-value and the ratio of UMP/uridine incorporated. This similarity suggests the presence of a ribosome bound RNA-dependent RNA polymerase activity in intact cells.
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Triplex formation as monitored by EPR spectroscopy and molecular dynamics studies of spin -probe -labeled DNAs
… (2,2,5,5-tetramethyl-3-ethynylpyrrolidine-1-oxyl)-uridine, which has been previously used in our laboratory to monitor triplex formation. Because of the difficult synthetic steps involved in the synthesis of this particular probe a new spin labeled DNA base, …
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Simulated impact trauma and osteoarthritis: the role of cell volume and mechanotransduction
… for stretch-activated calcium channels), uridine-5’-triphosphate (uridine; calcium mobilizing mediator) and the phosphoinositide 3-kinases (PI3K) inhibitor wortmannin were used to determine its mechanism of action. Wortmannin alone increased cell death and inhibited REV5901 …
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Characterization of 50S Ribosomal Subunit Assembly Inhibition in Erythromycin Treated <em>Escherichia coli</em> Cells.
… has been investigated using <sup>3</sup>H-uridine pulse-chase labeling of control and erythromycin treated <em>E. coli</em> cells.</p> <p>Subunit assembly was examined by sucrose gradient centrifugation of labeled cell lysates. Normal assembly kinetics of subunit assembly were observed in …
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High pressure liquid chromatography of nucleotides and nucleic acid bases from fungi
… than purines by a factor of at least three, with uridine nucleotides often representing 60- 80 mole percent of the total nucleotides. Extractable cytidine nucleotides are in negligible concentration in these species. Uridine diphosphoryl 2-acetamido-2-deoxy-α-D-glucopyranoside dominates all other …
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