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Showing 1 to 20 of 63 for “"unwinding"”.
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Unwinding Duchamp: Mots et Paroles a Tous les Etages
Practice is defined as the exercise of a method or profession, and a treatise. Practise is defined as to practise tricks or artifices upon; to act upon by artifice so as to induce to do or believe something: to impose upon, delude: to make trial of, practically. The practise that my practice …
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Critical role of histone tail entropy in nucleosome unwinding
… by determining the free energy cost of its DNA unwinding. Simulation results quantitatively reproduce thermodynamic parameters estimated from single-molecule force spectroscopy experiments, and capture the appearance of a large energetic barrier as the system transitions from the outer to the …
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Blaschke Decompositions on Weighted Hardy Spaces and the Unwinding Series
… in signal analysis, referred to as either the unwinding series or adaptive Fourier decomposition. In these processes, a signal is represented as the real component of the boundary value of an analytic function F : ∂D → C, and by performing an iterative method to obtain a sequence of Blaschke …
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Single-molecule optical tweezers studies of the mechanism of DNA unwinding by a model helicase
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-09-01 without embargo terms
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Functional characterization of Pura in vivo and in vitro - Pura knock-out mice and Pura DNA unwinding activity
… Pura displayed a helix destabilizing activity by unwinding a labeled 17-mer oligonucleotide annealed to ssM13 DNA. This activity was independent of ATP or any other nucleosides and lacks any directionality, distinguishing it therefore from common helicase activities. 16 truncated GST-Pura were …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
… A (RPA2). However, the mechanism by which unwinding enhancement occurs is unknown. In single-molecule optical trapping experiments, we monitor – with single base-pair precision – the unwinding of a DNA hairpin by XPD in the presence of RPA. We observe the effect of RPA2 on XPD unwinding in …
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Single-molecule optical trapping studies of the regulation mechanisms of XPD helicase
… play a critical role in this maintenance by unwinding DNA and RNA to enable replication, repair, and transcription. Harnessing ATP hydrolysis, helicases navigate complex nucleic acid structures, preserving genome stability and ensuring that genetic information remains accessible. Given their …
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XPD Helicase: Shifting the Inchworm Into Reverse
… (2) do helicases use the mechanism for unwinding as they use for translocation; (3) the role of unique insertions found among an otherwise conserved motor core; and (4) how these enzymes function as integral members of larger DNA processing machines. Here, I used the archaeal XPD …
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Single molecule fluorescence studies of replicative helicases
… translocation on single stranded (ss) DNA and unwinding of double stranded (ds) DNA. All of the 12 known replicative helicases are hexameric helicases that are ring-shaped in structure and are homohexamers with the exception of the eukaryotic minichromosomal maintenance (MCM) helicase. Their …
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Mechanism of Mcm10 function during DNA replication
… Cdc45 and GINS (forming the CMG complex). DNA unwinding cannot proceed, however, until an additional protein, Mcm10, associates with the CMG. Mcm10 continues to travel with the replication fork after stimulating initial DNA unwinding but a function for Mcm10 during DNA replication elongation …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
… force spectroscopy to study translocation and unwinding mechanism of Escherichia coli Rep, a model helicase with 3’5’ motor translocation activity on single‐stranded DNA (ssDNA) fueled by ATP hydrolysis. When a translocating Rep on a 3’‐overhang DNA reaches the DNA duplex junction, instead of …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
… force spectroscopy to study translocation and unwinding mechanism of Escherichia coli Rep, a model helicase with 3’5’ motor translocation activity on single‐stranded DNA (ssDNA) fueled by ATP hydrolysis. When a translocating Rep on a 3’‐overhang DNA reaches the DNA duplex junction, instead of …
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Single molecule studies of helicase mechanisms
… coli. Besides the canonical helicase function of unwinding duplex DNA, the RecQ family of helicases is known to preferentially bind to non-canonical DNA structures such as G-quadruplexes and Holliday junctions. Ensemble biochemical research has shown that BLM probably has roles in the maintenance …
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Mechanism and importance of Mcm2-7 double-hexamer formation during DNA replication initiation
… by helicase-activation proteins to initiate DNA unwinding. Importantly, this event is the committed step of replication initiation. Loading of two helicases in the head-to-head double hexamer ensures DNA unwinding on both sides of the origin and allows the assembly of bi-directional forks …
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Direct observation of XPD helicase base-pair stepping and regulation by RPA2
… of the genomic integrity. First, we examined DNA unwinding by XPD helicase in isolation to understand the basic physicochemical process of DNA base pair (bp) separation. We demonstrated that monomeric XPD unwinds duplex DNA in single base-pair steps, yet is non-processive, unwinding for short …
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Design criteria and equations of motion for the de-spin of a vehicle by the radial release of weights and cables of finite mass
… by the use of weights attached to the ends of unwinding cables of finite mass that are released when colinear with a radius of the payload.
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Single molecule investigations into DNA replication, recombination and repair
… indicating its specificity for Rad51. Srs2’s DNA-unwinding activity is greatly suppressed when Rad51 filaments form on duplex DNA. Taken together, our results reveal an exquisite and highly specific mechanism by which Srs2 regulates the Rad51 filament formation. Trinucleotide repeat (TNR) …
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Keeping It Together and Taking It Apart: Structural Investigations of Replisome Complexes Involved in DNA Replication Fork Protection and Termination
… cancer. Broadly, DNA replication involves the unwinding of the DNA double-helix, allowing DNA polymerases to use each unwound strand as a template for nascent DNA synthesis; the complex molecular machinery responsible for DNA replication is known as the replisome. However, during unwinding and …
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The Biology and Biochemistry of PhoH2 proteins
… show ATP/Mg²⁺ dependent, sequence specific RNA unwinding and cleavage. The sequence (A C) (A/U) (A/U) (G/C) U was deduced as a substrate for PhoH2, and PhoH2alt MTB also demonstrated unwinding and cleavage activity on its upstream ~152 base RNA transcript, suggesting a potential autoregulatory …
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