Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"unreplicated DNA"”.
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The Dynamics of the Unreplicated DNA Checkpoint in Xenopus laevis Embryos and Extracts
When unreplicated or damaged DNA is present, cell cycle checkpoint pathways cause cell cycle arrest by inhibiting cyclin-dependent kinases (Cdks). In Xenopus laevis, early embryonic development consists of twelve rapid cleavage cycles between DNA replication (S) and mitosis (M) without checkpoints …
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A model of the checkpoint response of the cell cycle of frog-egg extracts in the presence of unreplicated DNA
… consists of alternation between growth and DNA replication (interphase), and DNA distribution and cell-division (mitosis or M-phase). This process is regulated by a complex network of biochemical reactions. A core part of this network, called the "Cell Cycle engine" is evolutionarily …
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Loss of Chk1 Function and Exogenous Expression of Cyclin A1/Cdk2 Results in Apoptosis after the MBT in Early Xenopus laevis Embryos
… pathways to arrest cell cycles in response to DNA assaults. There is no transcription or apoptosis during these cell cycles, and they continue unperturbed until the 12th cell cycle, marking a period called the midblastula transition (MBT). At the MBT, the embryo undergoes a period of …
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Human common fragile sites, identification, and analysis of breaks using <i>saccharomyces cerevisiae</i>
… before mitosis and eventual breakage of unreplicated DNA. To test these hypotheses, we mapped 30 break locations in each of two yeast artificial chromosomes (YACs) containing human DNA inserts from fragile sites FRA3B and FRA7H. We compared break locations with the locations of high …
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The genome-wide analysis of the specification and activation of human chromosomal DNA replication origin sites
Background: Human DNA replication is a critical cellular process that initiates at tens of thousands of DNA replication origins. Next-generation sequencing (NGS) enabled the elucidation of some characteristics that define human replication origins. Initiation-site sequencing (iniSeq) utilises the …
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Quantitative Analysis of a Cell Cycle Checkpoint in Xenopus laevis Cell-Free Egg Extracts
… trigger cell cycle arrest in response to unreplicated or damaged DNA by inhibiting the activity of cyclin-dependent kinases (Cdks). In the Xenopus laevis embryo, checkpoints are not operational until the midblastula transition (MBT). Studies in cell-free egg extracts indicate that a …
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Post-Replicative Resolution of Under-Replication
… fork stall), which result in a segment of unreplicated DNA, was modelled using high quality origin-mapping data in HeLa and IMR-90 cells. From this, hypotheses were generated that related an increase in unreplicated segments of DNA to reduced functional origin number. Presented in this …
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Role of Chk1 and Chk2 in mitotic checkpoint control in vertebrate cells
… of mitotic checkpoint delay in response to DNA damage or incompletely replicated DNA is conventionally thought to be accomplished through inhibition of the cyclin-dependent kinase, Cdc2. Both Chk1 and Chk2 have the potential to operate in this pathway. Therefore, the initial aim of this …
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Establishing how bacterial cells position the division site
… to the progress of the initiation stage of DNA replication, such that the frequency of Z rings at midcell increases as this stage of DNA replication is progressively completed. Moreover, this link was found to be nucleoid occlusion independent. Spatial and temporal control of Z ring assembly …