Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"uncultured bacteria"”.
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Natural Product Biosynthesis In Uncultured Bacteria
… gram of soil can contain thousands of unique bacterial species, only a small fraction of which is regularly cultured in the laboratory. Although the fermentation of cultured microorganisms has provided access to numerous bioactive secondary metabolites, with these same methods it is not …
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Actinomycete biodiversity assessed by culture-based and metagenomic investigations of three distinct samples in Cape Town, South Africa
All of the samples used for actinobacterial isolation were subjected to a culture-independent (metagenomic) study. The results provided an explanation for why no actinomycetes were found in the aquatic samples, as all of the sequenced clones were shown to be most closely related to uncultured …
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Novel Molecules from Environmental DNA-Derived Type II Polyketide Biosynthetic Gene Clusters
… years. In fact, the origins of nearly 70% of antibacterial, antifungal, antiparasitic and antiviral small molecule drugs are in natural products (Newman and Cragg 2007). A significant proportion of natural product drugs and leads have been isolated from microbes (Newman and Cragg 2007). However, …
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Screening for novel cold-adapted nitrile hydratases in Antarctic metagenornic libraries
… been isolated from mesophilic and thermophilic bacteria. The aim of this study was to identify a novel cold-adapted NHase from psychrophilic and psychrotrophic bacteria. The identification of a cold-adapted NHase is of interest for its potential use in chemical and pharmaceutical production. …
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The Application of Functional Metagenomics to Natural Products Research
<p>The study of bacterial natural products offers important insights into the physiologies and behaviors of individual species of bacteria as well as the contributions of these organisms to complex microbial communities. Such information frequently has important downstream applications in medicine, …
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Metagenomics-Based Tryptophan Dimer Natural Product Discovery and Development Pipeline
… product discovery programs rely on the growth of bacteria in the laboratory, yet it is now well established that the vast majority of bacteria in the environment have not been cultured, particularly from the diverse soil microbiota. By extracting DNA directly from soil samples to construct large …
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Molecular characterization of Flavobacterium spp. isolated from Rainbow trout (Oncorhynchus mykiss) farmed in Southern Africa and development of a PCR-based tool for differentiating between isolates
Bacterial fish diseases caused by yellow-pigmented, filamentous bacteria of the genus Flavobacterium are among those that lead to significant losses in the international aquaculture industry. An increasing number of Flavobacterium spp. have been isolated in association with diseased fish within the …
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Efforts Toward Production of Novel Natural Products From Uncultured Soil Microbes
… of small molecule natural products from bacteria has led to the identification of many of the antibiotics currently in use today. Within the last 25 years, in fact, natural products (and their derivatives) account for almost three quarters of all antibiotic discoveries (Newman and Cragg). …
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Natural Products From Functional Screening of Soil Metagenomic Libraries
<p>Bacteria are a prolific source of therapeutically and industrially relevant natural products. Traditional routes of natural product discovery focus on cultured bacteria, a strategy that eludes 99% of the microbiome. Metagenomics offers a route to study uncultured bacteria by extracting DNA …