Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"uncoating"”.
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Differential modulation of HIV-1 capsid uncoating kinetics revealed by a novel single-particle uncoating assay
… capsid disassembles, in a process referred to as uncoating, with temporal and spatial precision. HIV has evolved to exploit a network of host cofactors to regulate capsid stability while the cell has developed restriction factors that recognise the CA lattice to block infection. Despite of that, …
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Investigation of the Vesicular Stomatitis Virus Matrix Protein: Uncoating and Assembly
… it is multifunctional and is involved in uncoating, cytopathic effects (CPE), and assembly of the virus. M protein interactions involved in the uncoating and assembly of VSV have been examined in this dissertation. <br /><br />Uncoating of VSV involves dissociation of M from the …
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Development of an Assay to study the Kinetics of HIV-1 Capsid Uncoating
… resistance to therapeutic intervention. Capsid uncoating is the process by which p24CA proteins (CA) disassemble from the viral ribonucleoprotein during the early phase of the HIV-1 lifecycle. Despite intensive investigation, much is yet unknown about the spatial and temporal occurrence of this …
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Steps to Reconstitute in vitro a Complete Round of COPI vesicle Budding, Uncoating and Fusion
… a program to reconstitute one round of budding, uncoating and fusion of a COPI vesicle from defined components, the next step is to include in a liposomal system the components needed for the fusion reaction. For that purpose, SNAREs (Soluble N-Ethylmaleimid-sensitive fusion protein Attachment …
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Host Proteins Interact with the Hiv-1 Core to Facilitate and Restrict
… infection. However, the precise process of HIV-1 uncoating is still unknown. Studies suggest that the process of uncoating is modulated by viral and cellular factors. Previously, HIV-1 was shown to traffic on microtubules, in a dynein and kinesin dependent mechanism. However, key host proteins …
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Evading Innate and Adaptive Immunity During Adenovirus Cell Entry
… suggest that adenovirus type 5 (Ad5) capsid uncoating occurs at the cell surface upon binding to both the coxsackievirus and adenovirus receptor and α<sub>v</sub> integrins. This uncoating event leads to the exposure of the capsid membrane lytic protein VI. Using the cytosolic protein …
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Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans
… which we hypothesize to be involved in vesicle uncoating and/or in modifying membrane phospholipids. SAC-2::GFP is localized to punctate structures along the ventral nerve cord and in the neuron soma. SAC-2::GFP localization to puncta in the soma is enhanced in rab-6.2(ok2254) null mutant …
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The Interferon Stimulated Gene Product Lymphocyte Antigen 6 Complex, Locus E Promotes Entry of a Subset of Diverse RNA Viruses and Inhibits Infection by Coronaviruses
… effect of LY6E specifically to the entry step of uncoating, which precedes release of the viral genome into the cytoplasm and is required for viral replication. I also observed that the viral enhancement phenotype is conserved across evolution, as orthologs from bat, rhesus macaque, and mouse …
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Principles of COPII Coat Assembly
… domain features to potentially stimulate uncoating and thus permit membrane fusion. An interface generated through multiple transient interactions highlights the capacity for their utilisation by sequence-divergent proteins to compete with the coat components during coat regulation, or to …
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Cryo-EM reveals how adeno-associated virus engages its receptor AAVR and an investigation of the GPR108 ectodomain
… are still unknown factors in AAV cell entry, uncoating, and host protein interactions. Furthermore, its use in gene therapy can be optimized with more accurate cell targeting and immune evasion. Being a non-enveloped virus, the protein shell of AAV is responsible for all of the host …
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Xylan chain length and plant cell wall assembly
… residues underwent conformational changes upon uncoating. Xylan binds cellulose, and xylan glucuronic acid branches crosslink xylan and lignin. Then, does xylan indirectly connect cellulose and lignin? I found that plants with short and scarce xylan were more severely stunted when their xylan …
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Charakterisierung einer Hüllprotein-vermittelten Restriktion von HIV-2 in humanen Zellen
… Verständnis von Post-Fusions-Ereignissen wie Uncoating, Zusammenlagerung des Präintegrationskomplexes und Start der reversen Transkriptase beitragen, die bislang nur unzureichend erforscht sind.
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Studio dell’attività antivirale e/o anti-infiammatoria di nuove molecole di sintesi e/o di farmaci noti
… that compound 7a interfered with the penetration/uncoating stage of the virus cycle. Conclusion: Further experiments to confirm the mechanism of action of the compoundare ongoing. Part II - In vitro studies on the anti-inflammatory activity of statins during neuroinflammation Aim: Due to the known …
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The Structural Basis of Flaviviridae Interaction with Antibodies and Receptors
… influence the assembly, receptor interaction and uncoating of virions. St. Louis Encephalitis Virus: SLEV) is another member of the JEV serocomplex with similar pathogenesis to JEV. We determined the 4.0 Ã… structure of the SLEV E protein in the post-fusion trimer conformation to compare it with E …
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Causes and Consequences of Genetic Robustness and Fragility in HIV-1 Proteins
… to preserve roles in viral assembly, maturation, uncoating and nuclear import, but is also under intense immunological pressure to diversify, particularly from adaptive immune responses. To evaluate the genetic robustness of HIV-1 CA, we generated a large library of random single amino acid …