Global ETD Search
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Showing 1 to 6 of 6 for “"ultrahigh-throughput screening"”.
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Strategies to Roam Enzymatic Fitness Landscapes in Ultrahigh-Throughput
Ultrahigh-throughput screening of enzymes enables us to explore large regions of sequence space – the theoretical universe of all possible enzyme variants for a given protein function. Each theoretical enzyme has a fitness value; peaks for high activity variants, and valleys for inactive enzymes …
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Directed evolution of amino acid dehydrogenases for biocatalysis of chiral amines
… is the development of technologies allowing the screening of large libraries of enzyme variants to explore sequence space efficiently. Massive scale-down of assay volumes by compartmentalization of library members in water-in-oil emulsions has recently led to the development of …
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Exploring protein fitness landscapes with new high-throughput technologies
… up protein engineering. In this study, high-throughput technologies will be developed that enable the read-out of directed evolution on a large scale, tracking the exploration of the valley of a fitness landscape: the conversion of an amino acid- to amine dehydrogenase will be investigated as …
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Towards ultrahigh-throughput phenotypic screening using microfluidic droplets and functionalised polyacrylamide beads
… and efficient core platform on which to build. Ultrahigh-throughput screening offers researchers a powerful tool to begin sampling larger regions of sequence space, and thus partially addresses one of evolution’s main advantages. This approach has particularly grown in promise and scale as DNA …
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Functional metagenomic discovery and characterisation of CAZymes by microfluidic methods
… of the protein landscape. Experimental screening for new enzymes based on actual catalytic turnover is the most direct way to new biocatalytic function without relying on homology to already known sequences. However, traditional wet lab-based work for the functional discovery and …
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
… are incredibly rare (1 in 105 on average). Ultrahigh-throughput droplet microfluidics has emerged as an effective technology to overcome this limitation, with the ability to screen 107 reactions per day. However, fewer than ten enzyme assays have been implemented in droplets to date and only …