Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 68 for “"ubiquitylation"”.
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Sortase-mediated ubiquitylation of Histone H2B and its biological consequences
… of cell biological processes. Histone 2B (H2B) ubiquitylation has been suggested to collaborate in the complex mechanisms that control the activation or silencing of genes. Here we try to explain how we could use the sortagging technique to control ubiquitylation events inside the nuclear …
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ERK1/2 signalling and protein ubiquitylation in the control of apoptosis
… and this serves to drive its K48-linked polyubiquitylation and proteasome-dependent degradation, thereby promoting cell survival. βTrCP, an F-box protein that acts in a larger SCF complex, is one of several E3 ligases that have been proposed to polyubiquitinate BIM. This study demonstrated …
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In vitro reconstitution of the ubiquitylation and disassembly of the eukaryotic replisome
… post-translational modification in the form of ubiquitylation on one of the subunits of CMG, leading to its disassembly from the chromatin.<br/><br/>Though the main players in the disassembly of CMG were known, viz the E3 ligase SCFDia2 and segregase Cdc48, very little was known about the …
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Investigating the role of protein ubiquitylation in the maintenance of genome stability
Homologous recombination (HR) is one the main mechanisms for repairing DNA double strand breaks (DSBs), as well as being a key downstream step in the repair of other DNA lesions, such as interstrand crosslinks (ICLs). The importance of HR for maintaining genome stability is underscored by the …
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Using "Designer" Nucleosomes to Study Enzymatic Crosstalk Between Histone Ubiquitylation and Histone Methyltransferases
… of direct enzymatic crosstalk between histone ubiquitylation and H3 lysine 79 methyltransferase, Dot1L, by investigating the plasticity of ubiquitin position in stimulation of Dot1L methyltransferase activity. Finally, using designer mononucleosomes containing H3 phosphoserine mimetics, …
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Ubiquitylation regulates vesicle trafficking and innate immune responses on the phagosome of inflammatory macrophages
… modifications. Here I explored the role of ubiquitylation in the phagosome biogenesis of Interferon-gamma (IFN-ɣ) activated macrophages. Ubiquitylation is a diverse, reversible post-translational modification which is not only involved in protein degradation but also in vesicle trafficking …
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The Role of Histone H2B Ubiquitylation and its Related Factors in Transcriptional Regulation in Mammalian Cells
… recent studies in yeast also have implicated H2B ubiquitylation in the transcription of specific genes. However, a systematic study of H2B ubiquitylation in mammalian cells has been hindered by the lack of information about mammalian homologues of the yeast enzymes responsible for H2B …
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Covalent modification and intrinsic disorder in the stability of the proneural protein Neurogenin 2
… Firstly I will describe work focused on ubiquitylation of xNgn2, targeting it for proteasomal degradation. xNgn2 is ubiquitylated on lysines, the recognized site of modification. I will discuss the role of lysines in ubiquitylation and stability of xNgn2. In addition to canonical …
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Functional analysis of the histone variant H2A.Z during lineage commitment
… we focused on the Polycomb-mediated mono-ubiquitylation of H2A.Z. We found that this modification regulates the differentiation potential of mouse embryonic stem cells (mESCs). Loss of H2A.Z ubiquitylation leads to a disrupted chromatin state and derepression of key developmental …
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Functional analysis of Shigella encoded IpaH E3 ubiquitin ligases in cell-autonomous immunity
… motility and cell-to-cell spread. So far, ubiquitylation of bacteria has mostly been associated with anti-bacterial autophagy or immune signaling. However, the ubiquitin coat assembled around intracellular Shigella by IpaH effectors, in particular IpaH9.8, serves a pro-bacterial function, …
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Modulation of Transcription Factor Activity by Mono-Ubiquitin
… domain of the activator. Remarkably, mono-ubiquitylation of the activator blocks this repressive activity. In this study, we probe the mechanism of this protective effect. Using novel label transfer and chemical cross-linking techniques, we show that ubiquitin contacts the ATPase complex …
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Proteasome Inhibition Enhances The Effect of Bikdd Gene Therapy
… for BikDD degradation revealed that both ubiquitylation dependent and ubiquitylation independent pathways contribute to BikDD degradation. High proteasome activity might be a potential mechanism for both intrinsic and acquired resistance in BikDD gene therapy, and combinational therapy …
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The ubiquitin coat of cytosol-invading bacteria
… Labelling, a novel tool to artificially induce ubiquitylation of S. flexneri by enforcing the recruitment of a Salmonella-targeting E3 ligase to the surface of Shigella. I demonstrate that SPLINT Labelling with LUBAC induces a M1-linked ubiquitin coat on S. flexneri and mediates the recruitment …
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Origins and composition of the cullin-RING E3 ligases in trypanosomes
Ubiquitylation is a post-translation modification essential for protein homeostasis that controls the fate of the targeted proteins by inducing their degradation, controlling enzymatic activity or changing cellular localisation following the covalent attachment of ubiquitin to a lysine residue of …
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Structural characterization of interaction of K11 and K63 mixed-linkage polyubiquitin chains with the tUIMs of epsin1
Ubiquitylation or covalent attachment of ubiquitin (Ub) to a variety of substrate proteins in cells is a versatile post-translational modification involved in the regulation of numerous cellular processes. The distinct messages that polyubiquitylation encodes are attributed to the multitude of …
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Fragment-based approaches to probe the surface of the VHL E3 ligase
… for specifically recognising substrates for ubiquitylation. Another strategy of increasing interest consists of developing bivalent molecules capable of bringing an E3 ligase in close proximity to a desired protein, inducing the ubiquitylation and subsequent degradation of an unnatural …
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Suppressor of cytokine signalling 3 (SOCS3) turnover and regulation of human saphenous vein smooth muscle cell signalling and function
… all 9 Lys residues that are potential sites of ubiquitylation to Arg should produce a mutated SOCS3 resistant to ubiquitin-mediated proteasomal degradation (“Lys-less” SOCS3). This study hypothesise that enhancing SOCS3 stability and limiting JAK/STAT signalling may provide sustained inhibition …
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Suppressor of cytokine signalling 3 (SOCS3) turnover and regulation of human saphenous vein smooth muscle cell signalling and function
… all 9 Lys residues that are potential sites of ubiquitylation to Arg should produce a mutated SOCS3 resistant to ubiquitin-mediated proteasomal degradation (“Lys-less” SOCS3). This study hypothesise that enhancing SOCS3 stability and limiting JAK/STAT signalling may provide sustained inhibition …
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Interlocking mechanisms regulating the circadian clock response to DNA damage
… of p53. In addition, MDM2 targets PER2 for ubiquitylation and degradation in a phosphorylation-independent fashion. Lastly, our studies showed that MDM2 collaborates with β-transducin repeat-containing proteins (β-TrCPs), an E3 ligase that targets PER2 for ubiquitylation in a …
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