Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 311 for “"ubiquitination"”.
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Decoding Lysine-11 Signals in Ubiquitination
The diverse outcomes of ubiquitination primarily relate to the flexibility of ubiquitin in forming homo- or heterotypic chains on each of its seven lysine residues which in turn stimulate distinct downstream signaling pathways. These ubiquitin signals must be selectively initiated on the substrate …
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Structural Insights into TRIM21 Ubiquitination Mechanisms
… essentially all physiological processes. During ubiquitination, RING E3 ligases direct E2 Ub-conjugating enzymes to substrates to catalyze their ubiquitination. The cytosolic antibody receptor TRIM21 possesses unique ubiquitination activity that drives broad-spectrum anti-pathogen targeting and …
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Signal Specific Ubiquitination and Degradation of IkBa
… this pathway in vitro by using the purified ubiquitination and degradation machinery to degrade IkBa, and activate NF-kB. While signal dependant IkBa ubiquitination was achieved early in the project, this substrate was not degraded by a number of different 26S protein preparations. Instead, …
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Proviral Roles of Ebola virus VP35 Ubiquitination
… proviral, but we did not know which VP35 role(s) ubiquitination regulated. We generated recombinant EBOVs encoding glycine (G) or arginine (R) mutations at VP35/K309 (rEBOV-VP35/K309G/-R) and show that both mutations prohibit VP35/K309 ubiquitination. The rEBOV-VP35/K309G mutant loses the ability …
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SORTASE AND CHEMICAL BIOLOGY AS TOOLS TO STUDY UBIQUITINATION
… still surround the impacts and roles of protein ubiquitination due to limitations in the current technologies available to study the role of this common post-translational modification. To address limitations in this field, we leverage the power of sortase, a bacterial transpeptidase enzyme …
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Biochemical Mechanism of Protein Kinase Activation by the Ubiquitination System
… cascade by TRAF6 and Ubc13/Uev1A in a polyubiquitinationdependent manner. Mechanistically how TAK1 is activated by the TRAF6- Ubc13/Uev1A dependent polyubiquitination system is unknown. Whether TAK1 and IKK kinases can be activated by other than TRAF6-Ubc13/Uev1A is an open question. By …
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Studies of E2s Related to Unconventional Ubiquitination in Arabidopsis thaliana
Protein ubiquitination, a fundamental protein modification cascade, is catalyzed by three types of enzymes referred to as E1 for ubiquitin (Ub) activation, E2 for Ub conjugation and E3 for Ub ligation. Arabidopsis has 37 E2 genes and functions for most of them remain largely unknown. Further, …
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An investigation into the role of ubiquitination in plant immunity
Plants have developed elaborate defence mechanisms to protect themselves against pathogens. Recently, the ubiquitin-proteasome pathway has been proven to play important roles in regulating plant disease resistance. Previously, the tobacco (Nicotiana tabacum) ACRE276 and its Arabidopsis homolog …
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Histone Methylation and ubiquitination as epigenetic regulators of plant development.
Τα φυτά προσαρμόζονται συνεχώς στο περιβάλλον τους ανταποκρινόμενα σε διάφορα εγγενή και εξωτερικά σήματα. Αντιμετωπίζουν πολυάριθμες βιοτικές και αβιοτικές πιέσεις, όπως ακραίες θερμοκρασίες, ξηρασία ή παθογόνα, που απαιτούν πολύπλοκους ρυθμιστικούς μηχανισμούς για τον έλεγχο της γονιδιακής …
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A study on PIP5Kalpha degradation by Nedd4-1-mediated ubiquitination
… proteasomal degradation via Nedd4-1-mediated polyubiquitination at lysine 88, which leads to reduction in PIP2 levels. Furthermore, we found that the mutation K88R enhances the inhibition of neurite outgrowth in Neuro2A cells. Our results suggest a functional link of Nedd4 family to modulation of …
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Investigations into the role of histone H2A ubiquitination in chromatin
… was used to determine the effect of histone H2A ubiquitination on linker histone binding to mononucleosomes. Hybrid octamers containing either H2A or ubiquitinated H2A (uH2A) were reconstituted onto random sequence 167 bp DNA. The affinity of the resultant nucleosome cores for linker histone H1 …
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Ubiquitination of EpoR and p85 in Ligand Induced EpoR Down-Regulation
… are poorly understood. Here we show that ubiquitination of EpoR controls internalization, lysosomal sorting, degradation and signaling of the EpoR. Ubiquitination of EpoR at K256 is necessary and sufficient for efficient Epo-induced receptor internalization, while ubiquitination at K428 …
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The Role of K63-Linked Ubiquitination Cycles In Akt Kinase Activation
… Akt membrane translocation is unclear. Protein ubiquitination is a significant posttranslational modification that controls many biological functions such as protein trafficking and signaling activation. Therefore, we hypothesize that ubiquitination may be involved in Akt signaling …
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Regulation of Diacylglycerol Acyltransferase-2 and Triacylglycerol Synthesis by Protein Ubiquitination
… that are ubiquitinated and determine the role of ubiquitination in regulating DGAT2 stability and triacylglycerol synthesis. Initial experiments found that a lysine-less DGAT2 mutant (Lys-less-DGAT2) was not degraded. Moreover, Lys-less-DGAT2 exhibited altered subcellular localization and …
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Role of the RING domain in MDM2-mediated ubiquitination of p53
… to the target protein, in the final step of the ubiquitination cascade. Various E2 enzymes have been shown to interact with their partner E3 ligases, yet evidence for the interaction between MDM2 and its partner E2, UbcH5α has not yet been shown. It has been reported that the reason for this lack …
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Proteolysis targeting chimeras for the directed ubiquitination of the androgen receptor
Proteolysis targeting chimeras (PROTACs) are an emerging field of therapeutics and promising potential drug candidates. PROTACs consist of a target protein binder connected via a linker to an E3 ligase binder. PROTACs hijack the ubiquitin proteasome system to degrade the target protein in a …
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Functional Interactions of E2~Ubiquitin Conjugates in and outside the Ubiquitination Pathway
… E3), the molecular details required for protein ubiquitination are largely unknown. To address questions regarding the final stages of Ub transfer, we began with structural characterization of the pathway intermediate, the E2~Ub conjugate (~ indicates the thioester linkage between the E2 active …
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Modulation of Kras Structure and Dynamics By Kras Ubiquitination and Membrane Depolarization
… and the membrane.</p> <p>KRAS undergoes monoubiquitination at two sites, Lys104 and Lys147. We observed that ubiquitin is quite dynamic around KRAS and forms weak non-specific interactions with KRAS. Through these interactions, it is capable of modulating KRAS dynamics to overcome deleterious …
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Ubiquitination of proteins involved in metabolism and immunomodulatory drug sensitivity in lymphocytes
… post-translational modifications, such as ubiquitination and phosphorylation, which can alter their activity, localisation and stability, making a cell responsive to its internal and external environment. Ubiquitin ligases and kinases comprise large enzyme families which catalyse such …
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