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Showing 1 to 11 of 11 for “"ubiquitin-like protein"”.
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Novel Insights into Ubiquitin-like Protein E1-E2 Interactions
… modification of macromolecules by ubiquitin-like proteins (UBLs) such as ubiquitin, Sumo and NEDD8 regulate a vast array of processes in the cell. Transfer of UBLs to their target generally occurs by a series of molecular handoffs down an E1‑E2‑E3 cascade. We are interested in …
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Identification and Characterization of a New E1 That Activates Ubiquitin and FAT10
Ubiquitination is one of many post-translational modifications in eukaryotes. Three enzymes (E1, E2, and E3) are involved in conjugating ubiquitin to protein substrates. I identified a novel E1-like protein, E1-L2, which is homologous to the ubiquitin E1 and another E1 involved in the activation of …
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ELUCIDATION OF THE ROLE OF FAT10 IN TUMORIGENESIS
… antigen F-associated transcript 10) is an 18kDA protein, which consists of 165 amino acid residues. It belongs to the ubiquitin-like modifier (UBL) family of proteins and encompasses two ubiquitin-like domains in tandem array. Since Ubiquitin is known to be involved in tumorigensis, we …
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Characterizing the Role of SUMO in Transcriptional Repression in S. cerevisiae
Small ubiquitin-like modifier, SUMO, is a member of the ubiquitin-like protein family. Similar to ubiquitin, SUMO is conjugated to target proteins as a post-translational modification via an E1, E2, and E3 enzymatic cascade, in a process known as SUMOylation. SUMOylation leads to altered target …
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Discovery of Novel Ubiquitin- and Methylation-Dependent Interactions Using Protein Domain Microarrays
… signal transduction by interacting with "reader" proteins. Protein domain microarray is a high throughput platform to identify novel readers for PTMs. In this dissertation, I applied two protein domain microarrays identifying novel readers for histone H2Aub1 and H2Bub1, and H3TM K4me3. …
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ISG15 conjugation : cellular targets and the lysine selectivity of HERC5
ISG15 is a 17 kDa ubiquitin-like protein that functions in the innate immune response to pathogens. While human ISG15 is a multifunctional protein, its canonical function is that of an intracellular ubiquitin-like protein modifier. Like ISG15, the human E1 (UBE1L), E2 (UBE2L6) and E3 (HERC5) …
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Analysis of markers of cell division cycle, apoptosis and autophagy flux in glioblastoma
… cycle; Cyclin A, an S-phase cyclin; Geminin, a protein that prevents re-initiation; and Phosphohistone H3 (PHH3), a marker of mitosis. Apoptotic markers included two anti-apoptotic proteins, Bcl-2 and Bcl-xl; a pro-apoptotic protein, Bak; and a final executioner caspase, caspase 3. Markers of …
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Regulators of autophagy in Leishmania major
… degradation pathway for recycling long-lived proteins and organelles that is thought to be required for life cycle progression and virulence of Leishmania. ATG8 is a ubiquitin like protein that is required for the formation of autophagosomes, and Leishmania uniquely possesses a set of …
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COP9 signalosome subunit knockdown in K562 provides novel insight into the function and potential regulation of the CSN
The co-ordinated degradation of proteins is vital to all aspects of cellular activity. The main mechanism of intracellular protein degradation is the ubiquitin proteasome system (UPS) which labels target proteins with ubiquitin, thereby marking them for degradation by the 26S proteasome. Protein …
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Biochemical and Structural Characterization of the Atg8/LC3 Lipidation Pathway
… eukaryotic orthologues LC3 and GARBARAP family proteins (referred here to Atg8 family proteins) play crucial roles in autophagy through their covalent ligation to lipids, typically phosphatidylethanolamine (PE), in a process known as lipidation. Lipidation of Atg8 family proteins regulates …
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Evaluation of drought tolerance in genetically modified Lolium perenne L. and Arabidopsis thaliana
Ubiquitin like proteins (UBLs) are known to have different functions including a potential role in drought tolerance of perennial ryegrass (Lolium perenne L.). UBLs are related in tertiary structure to ubiquitin (UB) and they both serve as regulators of many cellular processes. In previous work …