Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"ubiquitin conjugating enzyme"”.
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Novel insights into the post-translational regulation of an ER localised ubiquitin conjugating enzyme
Ube2J1 is an enzyme that helps cells degrade misfolded proteins at the endoplasmic reticulum. This study shows it is controlled by several signalling pathways, and regulated by previously unidentified mechanisms. Ube2J1 also boosts replication of Vesicular Stomatitis Virus when active. These …
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Differential regulation of the phosphorylated and dephosphorylated forms of the ubiquitin-conjugating enzyme Ubc6e
The ubiquitin proteasome system (UPS) is a key stress response employed by cells to remove damaged and misfolded proteins that accumulate in the endoplasmic reticulum (ER) during energy deprivation, hypoxia, or viral infection. Ubc6e is an ER-localised ubiquitin-conjugating enzyme that plays an …
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Using VHHs to study the function and control of the E2 Ubiquitin-conjugating enzyme UBC6e
… stress and at steady state. We found that an E2 ubiquitin-conjugating enzyme UBC6e contributes to a new layer of homeostatic control, in which ERAD activity itself is regulated post-transcriptionally and independently of the unfolded protein response. Ablation of UBC6e causes up-regulation of …
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Enhanced protein degradation by branched ubiquitin chains
… modification of cell cycle regulators with ubiquitin chains is essential for eukaryotic cell division. Such chains can be connected through seven lysine residues or the amino-terminus of ubiquitin, thereby allowing the assembly of eight homogenous and multiple mixed or branched conjugates. …
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Biochemical Mechanism of Protein Kinase Activation by the Ubiquitination System
… cascade by TRAF6 and Ubc13/Uev1A in a polyubiquitinationdependent manner. Mechanistically how TAK1 is activated by the TRAF6- Ubc13/Uev1A dependent polyubiquitination system is unknown. Whether TAK1 and IKK kinases can be activated by other than TRAF6-Ubc13/Uev1A is an open question. By …
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Key Role of Lys63-Linked Polyubiquitination in Viral Activation of IRF3
… procedures, Ubc5 was identified as a specific ubiquitin-conjugating enzyme (E2) involved in IRF3 activation. Taking advantages of inducible-RNAi strategy, catalytically active Ubc5 was shown to be essential for viral activation of IRF3. Furthermore, evidences were obtained indicating that …
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The Role of Two Homologous E3 Ligases In Muscle Physiology
<p>Ubiquitin-mediated proteasomal degradation is an essential cellular function that is coordinated by three key components: E1 ubiquitin activating enzyme, E2 ubiquitin conjugating enzyme, and E3 ubiquitin ligases. There are an estimated 600 E3 ligases, some of which share high sequence homology; …
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Biochemical and Genetic Studies on CC2D1A, a new NF-κB Activator and a Regulator of Synaptic Functions
… components in the canonical pathway including ubiquitin-conjugating enzyme UBC13, a RING domain ubiquitin ligase TRAF2, a protein kinase TAK1, and an essential regulator of IKK complex, NEMO. CYLD, a deubiquitination enzyme specific for Lysine-63 linked polyubiquitin chains, negatively …
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Investigating The Interaction of Aurka and Ube2C In Colorectal Cancer Cells
… study, one of the genes in the signature, the ubiquitin conjugating enzyme UBE2C, was identified through preliminary bioinformatics analysis as a candidate for further examination of its role in CRC progression. Co-expression analysis of UBE2C in tumor-normal datasets from the public database …
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Dual Roles for NBR1 in Bone Remodelling and Autophagosomal Protein Degradation
… UBA domain of Nbrl binds mono- and polyubiquitinated chains whilst the N-terminal PB1 domain interacts with the structurally similar protein p62. The aims of this thesis were to further characterise the biological functions of Nbrl by identifying novel protein interactors. A …
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Regulation of Protein Degradation by ADP-Ribosylation
… is essential for cellular homeostasis. The Ubiquitin-Proteasome System (UPS) is responsible for the regulated breakdown of intracellular proteins. All proteins are not degraded at the same rate in the cell. For instance, global protein turnover rates in mammals change with an average time …
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Investigating the role of protein ubiquitylation in the maintenance of genome stability
… HR factors. In this manner, I identify the E2 ubiquitin conjugating enzyme UBE2S as a novel factor required for olaparib resistance, and confirm its direct involvement in DNA repair by showing that it rapidly accumulates at sites of DNA damage. This work highlights UBE2S as a potentially useful …
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Epigenetic Regulation of Replication-Dependent Histone mRNA 3 End Processing
… globaler Mengen an Histon H2B Lysin 120 Monoubiquitinierung (H2Bub1) verantwortlich ist, sondern darüber hinaus auch für die Histonmodifikationen Histon H3 Lysin 4 Trimethylierung (H3K4me3) und H3K36me3 von Bedeutung ist. Allerdings scheinen diese Modifikationen nicht ausschließlich von der …
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Regulation of Protein Degradation at the Endoplasmic Reticulum
… as HMG-CoA Reductase (HMGCR), the rate limiting enzyme in cholesterol biosynthesis. Under sterol rich conditions, HMGCR is rapidly degraded through ERAD, however, when sterols are limited, HMGCR is stabilised through decreased degradation and increased transcription by the transcription factor …
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Lineage-Specific Phenotypic Abnormalities in iPSC-Derived Smooth Muscle Cells from Type-I Loeys-Dietz Syndrome
… effects of the mutation. Additionally, ubiquitin-conjugating enzyme E2 L3 (UBE2L3), a regulator of TGFBR1 degradation, was upregulated in LM-SMCs relative to NC-SMCs. Silencing UBE2L3 expression in LM-SMCs did not restore TGF-β signalling and mitochondrial functions, but modestly …
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Components of the Ubiquitin Proteasome System are Required for the Nonapoptotic Death of the C. Elegans Linker Cell
… Our work suggests that components of the ubiquitin proteasome system (UPS) act to promote linker cell death. We show that LET-70, an E2 ubiquitin-conjugating enzyme, is required cell-autonomously for linker cell death. LET-70 levels, as well as those of ubiquitin and some proteasome …
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The Role of Histone H2B Ubiquitylation and its Related Factors in Transcriptional Regulation in Mammalian Cells
… about mammalian homologues of the yeast enzymes responsible for H2B ubiquitylation. I report identification of a functional human homologue, the hBRE1A/B complex, of the yeast BRE1 E3 ubiquitin ligase. hBRE1A, which forms a complex with hBRE1B, specifically increases the global level of …
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Protein quality control in the mammalian endoplasmic reticulum
… then moved across the ER membrane, tagged with ubiquitin, and extracted from the membrane and complex. Finally, the dislocation substrate is taken to the proteasome where it is degraded. Many proteins constituting the mammalian quality control machinery have been identified. Several different …
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Molecular Regulation of Vascular Calcification In Murine Models of Atherosclerosis
… Wnt/β-catenin system and potentially GTAP, an ubiquitin-conjugating enzyme responsible for protein degradation, which participates in the homeostasis of proteins and metabolites in stem cells. The long-term goal of this study is to delineate the molecular pathway(s) by which ApoE regulates …
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The Role and Regulation of Id2 In Dendritic Cells
… genetic mouse models. TLR activation of the E2 ubiquitin-conjugating enzyme Ubc13, rapidly stimulates <em>Id2</em> transcriptional activity, independent of type I IFN (IFN-I) signaling in pDCs. Based on this mechanism, we further assessed roles for TLR agonists in related DC populations. We …
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