Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"uORFs"”.
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An inductive logic programming approach to learning which uORFs regulate gene expression.
Some upstream open reading frames (uORFs) regulate gene expression (i.e. they are functional) and can play key roles in keeping organisms healthy. However, how uORFs are involved in gene regulation is not het fully understood. In order to get a complete view of how uORFs are involved in gene …
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Comparative analysis of African and Asian/American Zika virus strains at the transcriptional and translational level
… overlooked upstream open reading frames (uORFs) in the 5′ untranslated region (UTR) of the American ZIKV genome were identified, initiating at non-canonical CUG and UUG codons; and a single uORF in the 5′UTR of the African ZIKV, initiating at a CUG start codon. The uORFs in the American …
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The response of eIF2α kinases to plant virus infection
… regulates translation of mRNAs with particular uORFs. To determine whether this type of regulation occurs in plants, a Luciferase/Renilla reporter system and yeast general control non-derepressible-4 (GCN4) uORFs were used. The uORFs did not regulate the translation of a main reporter ORF in …
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The Regulation of NAP4 in Saccharomyces cerevisiae
… via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which …
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Understanding regulation of mRNA by RNA binding proteins
… translated upstream open reading frames (uORFs) are enriched in transcription factor mRNAs and predict mRNA repression by NMD, while poorly translated mRNAs escape repression.
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Mitochondrial parts, pathways, and pathogenesis
… widespread role of upstream open reading frames (uORFs) in blunting translation of mitochondrial and other cellular proteins. Next we used the mitochondrial protein inventory to identify genes underlying inherited diseases of mitochondrial dysfunction. In collaboration with clinicians, we …
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Characterization of KPAP, a Novel Pokeweed Antiviral Protein Isoform
… of predicted upstream open reading frames (uORFs) in the long leader intron, perhaps belonging to retrotransposons. The enzymatic activity recorded from KPAP in an in vitro translation assay resembles the activity of PAP-I. Moreover, the exclusive expression of KPAP in leaves compared to …
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Post-Transcriptional Regulation of Mammalian Gene Expression In Non-Coding Region of Target Rna
… that two small upstream open-reading frames (uORFs) in the 5’UTR of TUSC2 could markedly inhibit the translational initiation of TUSC2 protein by interfering with the “scanning” of the ribosome initiation complexes. Site-specific stem-loop array reverse transcription-polymerase chain reaction …
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The mechanisms regulating the transcription factor ATF5 and its function in the integrated stress response
… involves two upstream open reading frames (uORFs) located in the 5′-leader of the ATF5 mRNA, a feature shared with ATF4. Mutational analyses of the 5′-leader of ATF5 mRNA fused to a luciferase reporter suggests that the 5′-proximal uORF1 is positive-acting, allowing scanning ribosomes to …
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Studying sequence effects of mRNA 5' cap juxtapositions on translation initiation rate using randomization strategy of the extreme 5' end of mRNA
… initiation, it is possible that such as uORFs and RNA secondary structures, have a higher influence on translation initiation than E5S. The E5S is a previously unappreciated determinant of translation initiation, and this work suggests that differences in mRNA 5' end accessibility defined …
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RNA regulatory elements and translational control
… the possible existence of two additional uORFs. And finally, the work focussed on a plant RNA ThermoSwitch. I established a reporter-based experiment used to test the effect of ThermoSwitch sequences in a transient expression system in *N. benthamiana*. The results mark a step towards the …
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Gene misexpression in humans
… for specific sORF types including upstream ORFs (uORFs) and lncRNA-ORFs. Overall, the performance of the best models was modest achieving a median AUROC of 0.71, 0.74 and 0.60 for all sORFs, uORFs and lncRNA-ORFs, respectively. Analysing the contribution of different features to model performance …
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Development of a ribosome profiling protocol to study translation in the yeast Kluyveromyces marxianus
… open reading frames present in the 5’ leaders (uORFs). Ribosome profiling was initially developed in the model yeast S. cerevisiae, but has since spread to human and bacterial models. The first results chapter of this thesis describes the development of a ribosome profiling protocol to study …