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Showing 1 to 20 of 26 for “"type three secretion"”.
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Regulation of the Salmonella Pathogenicity Island 1 Type Three Secretion System
… enterica serovar Typhimurium is mediated by a Type Three Secretion System (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). The SPI1 TTSS injects effector proteins into the cytosol of host cells where they promote actin rearrangement and engulfment of the bacteria. HilC, HilD, and …
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Virulence associated functions and vaccine potential of the burkholderia mallei type three secretion effector BopA
… of a polysaccharide capsule and presence of secretion systems as the only known mechanisms associated with virulence. Various gram-negative pathogens utilize a secretion system known as type III, which is used to deliver bacterial (effector) proteins into host cells to modulate immunological …
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Structural and functional studies of type three secretion virulence factors from gram-negative pathogenic bacteria
Many pathogenic Gram-negative bacteria utilize type III secretion systems (TTSS) to alter the normal functions of target host epithelial cells. Of the 1.1 million deaths that are caused by Shigella each year, nearly a third are in children under five years of age. Salmonella enterica serovar …
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Tagging of the type three secretion system basal apparatus of Enterohaemorrhagic Escherichia coli 0157:H7
… Escherichia coli (EHEC) O157:H7 possesses a type III secretion system (T3SS). The T3SS translocates effector proteins into host cells during infection and consists of a basal apparatus that spans the inner and outer membranes and a needle and filament complex. The basal apparatus is composed …
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Structural characterization of chaperone and substrates from the type three secretion system of bacterium aeromonas hydrophilla
… humans. This gram-negative pathogen utilizes the Type Three Secretion System to transport effector proteins into the host cell. Several proteins are essential for the transportation of these effectors, they are the secretion needle subunit, AscF and pore forming translocators, AopB and AopD. Prior …
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The Chlamydia Trachomatis Protein Interaction Network: Insights into the Unique Composition of the Type Three Secretion System
… in modulating host cellular functions and the secretion of virulence factors into the host. Since the delivery of virulence proteins by a type three secretion (T3S) system is a critical step for Chlamydia, we identified the proteins that interacted with the T3S apparatus by yeast two-hybrid …
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The Role of Periplasmic Disulfide Bond Status in the Regulation of the Salmonella SPI1 Type Three Secretion System
… via the Salmonella Pathogenicity Island I (SPI1) type III secretion system (T3SS) to induce inflammatory diarrhea and bacterial uptake into intestinal epithelial cells. The master SPI1 regulatory gene hilA is controlled directly by three AraC-like regulators: HilD, HilC and RtsA. A periplasmic …
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Mechanistic Studies of the Roles of the Transcriptional Activator ExsA and Anti-activator Protein ExsD in the Regulation of the Type Three Secretion System in Pseudomonas aeruginosa
… injecting toxins into a host cell, called the type three secretion system (T3SS). The secretion machinery itself is highly complex and, together with the specific secreted factors, requires expression of more than 30 genes. Due to the high energy cost of its synthesis to the organism this …
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Analysis Of Yscf And Associated Homologs And The Presence Of An Inflammatory Molecular Pattern
… pathogen, Yersinia pestis, expresses a type three secretion system to accomplish its virulence. The YscF needle protein provides a conduit of effectors from bacterium to eukaryote and is exposed to the host immune system. This thesis explores YscF and its associated needle homologs and …
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Rational development of novel small molecule leads against the transcriptional activator protein ExsA of Pseudomonas aeruginosa
… to acute and chronic infections. P. aeruginosa’s type three secretion system (T3SS) dominate in acute infection, and the expression of this system is regulated by the transcription factor ExsA. The latter therefore represents an attractive target for developing anti-virulence drugs against this …
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Comparative Genomics of Selected Species of Gram-Negative Bacteria
… \(coli\), the distribution of two Type III secretion system, ETT2 (\(E.\) \(coli\) Type Three Secretion 2) and Flag-2 (\(E.\) \(coli\) Flagellar system 2), were surveyed among a collection of 79 strains. Remnants of both clusters were found to be present in most strains, suggesting …
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The impact of a single nucleotide polymorphism in fusA1 on biofilm formation and virulence in Pseudomonas aeruginosa
… of a major virulence determinant, the type three secretion system, typically characteristic of cells comprising an acute infection. Proteomic and transcriptomic profiling of the mutant strain provided an insight into the genetic basis behind these phenotypes, identifying the …
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Small molecule inhibitors against enteric bacterial glycosyltransferases
… Pathogenic bacteria evolved to develop various secretion systems to deliver virulence proteins termed ‘effectors’. These virulence factors promote the invasion of pathogens as well as evasion of host innate immune response. Type III secretion system (T3SS) is one of the most complex secretion …
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Coordinated regulation of the flagellar, Salmonella pathogenicity island 1, and the type I fimbriae gene networks in Salmonella typhimurium
… In addition, there is cross-talk between these three systems which serves to dynamically control the timing of activation and de-activation of these networks. Collectively, we demonstrate that cells dynamically process information in genetic networks which ensures that the encoded products are …
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The Characterization of the Fucose Sensing Kinase (FUSK) and the Fucose Sensing Response Regulator (FUSR) and Their Role in Virulence Regulation in Enterohemorrhagic Escherichia Coli O157:H7
… (LEE) that encodes for a molecular syringe, a type three secretion system (T3SS), which translocates effectors to the host cell. Expression of the LEE is controlled by the AI-3/Epi/NE interkingdom signaling cascade. The two-component systems QseBC and QseEF are at the core of the AI-3/Epi/NE …
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Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay
… humans and other vertebrates. To delineate pathotypes, the genomes of twenty-eight <em>Aeromonas </em>isolates<em> </em>were screened by PCR to determine the presence of virulence factors including: aerolysin (<em>aerA</em>)<em>, </em>cytotoxic enterotoxin (<em>act</em>), hemolysin …
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Investigation Of The Interconnected Roles Of Cmal And Hopaa1-1 In The Virulence Of Pseudomonas Syringae Pv.Tomato Dc3000 In Nicotiana Benthamiana
… the phytotoxin coronatine and the delivery of type III effector proteins (T3Es) into the host cell cytoplasm for defense suppression and virulence. CmaL is a small protein found to be necessary for coronatine production. Coronatine is a potent molecular mimic of jasmonoyl-isoleucine, a plant …
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Coordinate Regulation of Salmonella Virulence Gene Expression During Infection
… epithelial cells. Invasion is mediated by a Type Three Secretion System (TTSS), which is encoded on Salmonella Pathogenicity Island 1 (SPI1). The SPI1 TTSS forms a needle complex through which effector proteins are injected into the cytosol of host cells where they induce engulfment of the …
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The WXXXE Effector Map Functions as a Potent and Specific Guanine Nucleotide Exchange Factor for CDC42
Many gram negative bacterial pathogens utilize a type three secretion system (TTSS) in order to infect eukaryotic cells. A TTSS looks and acts like a molecular syringe, allowing bacteria to inject effector proteins directly into the host cell cytoplasm. These effectors commandeer and manipulate …
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Elucidating three novel mechanisms of Pseudomonas syringae pathogenicity
… P. syringae secrete effector proteins through a Type Three Secretion System (T3SS) to suppress the immune response elicited by their MAMPs. However, secretion of effectors can also trigger a strong plant immune response if the plant harbors resistance proteins capable of recognizing the secreted …
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