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Showing 1 to 20 of 22 for “"type VI secretion system"”.
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Characterizing the Role of the Type VI Secretion System in Interbacterial Species Interactions and Pathogenesis
… to properly sense and respond to their environment. This allows them to compete for specific niches, persist in polymicrobial communities, and contribute to virulence and pathogenesis. One mechanism Gram-negative bacteria have adapted is the use of type VI secretion systems (T6SS). The …
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Specialised Effectors and Accessory Proteins Important in the Antibacterial Type VI Secretion System of <i>Serratia marcescens</i>
Bacteria are often found in polymicrobial environments and, to ensure a fitness advantage, have adopted diverse strategies to fight other microbes within their preferred niche. An important mechanism utilised by Gram-negative bacteria is protein secretion, with bacterial secretion systems …
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The role of the Vibrio cholerae Type VI secretion system during kin cell interaction, biofilm formation and priority effects
<p><em>Vibrio cholerae’s </em>Type VI secretion system (T6SS) is one of the arsenals used by the bacteria for its long-term survival in different environments, against a variety of opponents, and for its maintained pathogenicity in vulnerable regions of the world. Therefore, the more we understand …
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Investigation into the transcriptional regulation and role in virulence of the Type VI Secretion System of <em>Serratia marcescens</em> Db10
The Type 6 Secretion system (T6SS) is the most recently identified secretion system in Gram-negative bacteria. It is widely distributed and has been found to target both eukaryotic and prokaryotic cells. The T6SS has been described as resembling an inverted bacteriophage cell puncturing device …
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Dissecting the puncturing device of the Type VI Secretion System: role of Hcp, VgrG and PAAR proteins in assembly and secretion of effectors
The Type VI secretion system (T6SS) is a macromolecular machinery, widespread in Gram negative bacteria, which is used to deliver toxic proteins (“effectors”) directly into target eukaryotic or prokaryotic cells. As a result, this system plays a crucial role both in direct pathogenicity and in …
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Important role of the type VI secretion system in the virulence of an emergent human pathogen Aeromonas hydrophila: Delineating the mechanism of action of its effectors
… In this study, we characterized the new type 6 secretion system (T6SS) from isolate SSU of A. hydrophila and demonstrated its role in bacterial virulence. We have provided evidence that the T6SS is independent of other secretion systems and that the vasH gene is essential for the …
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Structure of T6SS components and assessment of potential Gram-ve drug targets
… and potential function of two components of the type VI secretion system in Serratia marcescens have been investigated by X-ray crystallography. Chapters 1 and 2 describe the background of the type VI secretion system and experimental methods respectively. In chapter 3, the solution of the …
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Structural studies to inform antimicrobial drug discovery and the basis of immunity against T6 effectors
… acid on the path to folate. Comparisons with previously solved PabC structures identified a spatially conserved tyrosine residue in the active site and suggest that a re-evaluation of a published mechanism is warranted.<br/>Part two: Immunity proteins in the Gram-negative Type VI secretion …
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An analysis of selected secretion systems of Pseudomonas species
Direct secretion systems which deliver molecules from one cell to another have huge significance in shaping bacterial communities or in determining the outcome of bacterial associations with eukaryotic organisms. This work examines the roles of the Type III Secretion System (T3SS) and the Type VI …
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Exploring Interactions of Phyllosphere Epiphytes with Plant Pathogenic Bacteria Pseudomonas and Xanthomonas on Tomato
… this study uses X. <em>perforans</em> wild-type and X. <em>perforans</em> type VI secretion system (T6SS) mutant strains to observe the role of type VI secretion system in infection with a potential biocontrol agent. To explain interactions among the <em>phyllosphere</em> residents, …
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Characterizing the advantages of diverse effector secretion in Pseudomonas aeruginosa
In the environment, bacteria exist in complex communities with other bacterial species, where they compete for resources. Cells are frequently in close proximity to one another, and therefore, many bacteria have evolved pathways to intoxicate their neighbors directly. One such pathway is the type …
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Characterization of The Role of The Pkm101-Encoded T4Ss Outer Membrane Core Complex In Substrate Transfer, Pilus Biogenesis, and Recipient Cell Contact
<p>The bacterial type IV secretion systems (T4SS) encoded on the <em>Escherichia coli</em> pKM101 and R388 conjugative plasmids and the <em>Agrobacterium tumefaciens</em> <em>virB</em> operon are composed of 12 subunits arranged in an architecturally similar fashion. Structural studies of the T4SS …
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Combating Plague: Fighting a War on Two Fronts Through Vaccine Design and New Therapeutic Intervention
… against plague infection. Identification of new virulence factors of Y. pestis and understanding their role during the infection process is imperative in designing a better vaccine candidate. By using high-throughput signature-tagged mutagenic approach, we screened 5,088 mutants of Y. pestis CO92 …
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CHARACTERIZATION OF CA²⁺-MEDIATED VIRULENCE AND MICROBIAL COMPETITION IN THE PLANT PATHOGEN PSEUDOMONAS SYRINGAE
… evolved mechanisms to sense and respond to environmental Ca²⁺ levels, using these signals to fine-tune their physiology and coordinate virulence.Pseudomonas syringae, a model bacterial plant pathogen, uses a sophisticated arsenal of virulence factors, including the type III secretion system …
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Construction, characterization, and evaluation of CLH001 as a vaccine candidate against respiratory glanders
… (CLH001), a strain deficient in iron uptake and type six secretion functions, and investigated its ability to protect against acute respiratory glanders infection. When compared to wild-type (wt), CLH001 exhibited decreased growth kinetics in both culture media (LBG) and RAW 264.7 murine …
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Analysis of the diverse antibacterial strategies used by <i>Serratia marcescens</i> Db10
… properties, including antibiotics. The activity of NRPS and PKS enzymes is dependent on their modification by a phosphopantetheinyl transferase (PPTase) enzyme. In this work we identify the biosynthetic genes, <i>alb2-6</i>, which are required for the production of the NRPS-PKS antibiotic, …
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Loss of LasR function reshapes inter-species interactions in a polymicrobial cystic fibrosis airway model
… P. aeruginosa often has to share the airway environment with a variety of other microbes: these are polymicrobial infections. We now know that the interactions between co-habiting Gram-negative, Gram-positive, and fungal species can significantly impact the outcomes of these airway infections. …
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