Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"type III secretion system (T3SS)"”.
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Tagging of the type three secretion system basal apparatus of Enterohaemorrhagic Escherichia coli 0157:H7
… Escherichia coli (EHEC) O157:H7 possesses a type III secretion system (T3SS). The T3SS translocates effector proteins into host cells during infection and consists of a basal apparatus that spans the inner and outer membranes and a needle and filament complex. The basal apparatus is composed …
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Transcriptional Regulation of NLRC4 Inflammasome by IRF8
… which recognize bacterial flagellin and type III secretion system (T3SS) proteins. IRF8 was critical for host survival following infection with Salmonella Typhimurium or Burkholderia thailandensis. Furthermore, mice deficient in IRF8 were impaired in their ability to produce IL-18 and …
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An analysis of selected secretion systems of Pseudomonas species
Direct secretion systems which deliver molecules from one cell to another have huge significance in shaping bacterial communities or in determining the outcome of bacterial associations with eukaryotic organisms. This work examines the roles of the Type III Secretion System (T3SS) and the Type VI …
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Mechanism of action of AexU, a new type III secretion system effector from an emerging human pathogen Aeromonas hydrophila
… first reported the complete sequence of the type III secretion system (T3SS) from a diarrheal isolate SSU of A. hydrophila. We identified an effector protein (designated as AexU) of the T3SS, which exhibited ADP-ribosyltransferase (ADPRT) and GTPase-activating protein (GAP) activity. AexU was …
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The role of citrate in plant-pathogen interactions
… and as an inducer of genes associated with the type III secretion system (T3SS) and phytotoxin production. The effect of citrate on the host is less clear, though microarray analysis of Arabidopsis thaliana has demonstrated that application of exogenous citrate leads to the differential …
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The Role of Periplasmic Disulfide Bond Status in the Regulation of the Salmonella SPI1 Type Three Secretion System
… via the Salmonella Pathogenicity Island I (SPI1) type III secretion system (T3SS) to induce inflammatory diarrhea and bacterial uptake into intestinal epithelial cells. The master SPI1 regulatory gene hilA is controlled directly by three AraC-like regulators: HilD, HilC and RtsA. A periplasmic …
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Functional analysis of Shigella encoded IpaH E3 ubiquitin ligases in cell-autonomous immunity
… injected into the host cytosol via the bacterial type III secretion system (T3SS) to manipulate the host cell and counteract anti-bacterial defense pathways. My work demonstrated that IFN-induced guanylate-binding proteins (GBPs) are novel targets for IpaH9.8. GBPs inhibit actin-dependent motility …
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Comparative proteomic analysis of Australian epidemic Bordetella pertussis
… SS. THIJS was found to promote virulence factor secretion and is more suitable for studying the secretome. For surfaceome, optimal surface shaving of B. pertussis was performed with 1 µg of trypsin for 5 min. Flow cytometry demonstrated minimal cell lysis after shaving. The whole cell, secretome …
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THE BIPC MINOR TRANSLOCASE MEMBRANE PROTEIN OF THE BURKHOLDERIA TRANSLOCON
Some Gram-negative bacteria utilize type III secretion system (T3SS) as a tool in their arsenal in the pursuit of host invasion. The T3SS works to the advantage of the pathogen and acts like a syringe, cleverly bypassing any of the host immune responses, which injects effector proteins (virulence …
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CHARACTERIZATION OF CA²⁺-MEDIATED VIRULENCE AND MICROBIAL COMPETITION IN THE PLANT PATHOGEN PSEUDOMONAS SYRINGAE
… arsenal of virulence factors, including the type III secretion system (T3SS), virulence factors, and phytotoxins, to manipulate plant cellular processes and suppress immunity. Emerging evidence suggests that Ca²⁺ serves as an important environmental cue that modulates the expression and …
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Biochemical Characterization of IpaH E3 Ubiquitin Ligase Effector Proteins and Their Host Substrates
… is a gram negative pathogen that utilizes its type 3 secretion system (T3SS) to inject effector proteins in the cytoplasm of host cells to manipulate host cells processes. T3SS effectors are able to post translationally modify host proteins to reprogram intracellular signaling pathways, actin …
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Modulation of host phosphatidylinositol phosphates by salmonella effector protein SOPB
… island 1 (SPI1) encoded type III secretion system (T3SS), a needle-like complex composed of over 20 proteins that translocates effector proteins directly into the host cell cytosol. <em>Salmonella</em> possess a second type III secretion system encoded on …
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Characterizing induced gene expression in Shigella flexneri following bile salt exposure
… bile salts exposure. Here, a mutant and wild-type 2457T strains were used in infection of HT-29 colonic epithelial cells to compare invasion ability and intracellular replication. The Congo red (CR) secretion assay was also used as a measure of virulence protein secretion from the type-III …
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Molecular Interactions of Type III Secretion System Transcriptional Regulators in Pseudomonas aeruginosa: ExsA and ExsD
… the leading causes of nosocomial infections. The type III secretion system (T3SS) aids acute P. aeruginosa infections by injecting potent cytotoxins (effectors) into host cells to suppress the host's innate immune response. Expression of all T3SS-related genes is strictly dependent upon the …
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Protein glycosylation by NleB and the secretion of therapeutic nanobody fusions
… interact with mammalian hosts by using a type III secretion system (T3SS) to secrete effectors into host cells. NleB and SseK are two E. coli and Salmonella effector orthologs that are unusual glycosyltransferases. They glycosylate host protein substrates on arginine residues with N-acetyl …
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Post-Transcriptional Regulation of Virulence Genes by GlmY and GlmZ in Enterohemorrhagic E. Coli
… major virulence factors in this pathogen is a type III secretion system (T3SS) encoded by the locus of enterocyte effacement (LEE). EHEC uses this proteinaceous needle to inject effector proteins into host cells to hijack various host cellular processes, as well as the translocation and …
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Signaling Specificity in a Campylobacter jejuni Two-Component System to Mediate Proper Flagellar Gene Transcription
… genetic screens discovered the flagellar type III secretion system (T3SS), the FlgSR two-component regulatory system (TCS), and the FlhF GTPase as requirements to positively regulate expression of σ54-dependent genes that encode flagellar rod and hook proteins. Our laboratory previously …
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Biofilm and Virulence Regulation of the Cystic Fibrosis Associated Pathogens, Stenotrophomonas maltophilia and Pseudomonas aeruginosa
… are due to pressures from the host immune system and lead to permanently mutated strains, most likely an adaptive strategy to evade host immune responses. Biofilms are extremely problematic in a clinical setting because they lead to nosocomial infections and persist inside the host causing …
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Identification and characterization of type III secretion inhibitors in Erwinia amylovora, the causal agent of fire blight of apple and pear
… is the causative agent of fire blight. The type III secretion system (T3SS) and exopolysaccharide (EPS) amylovoran are two major yet separate virulence factors in E. amylovora. Current fire blight management mainly relies on application of copper compounds and antibiotics. However, …
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Structural study of ExsA, the regulator of Type III Secretion System of Pseudomonas aeruginosa
The Type III secretion system (T3SS) of Pseudomonas aeruginosa uses a needle-like protein apparatus to detect eukaryotic host cells and translocate effectors directly into the host cell. The effectors are also known as cytotoxins, which cause disruption of a series of signaling events in the host …
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