Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"two-state model"”.
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Das "Leucine-Rich Repeat" im Invasionsprotein Internalin B : Stabilität und Faltung eines Solenoidproteins
… was die Anwendung eines Zweizustandsmodells zur Beschreibung der Daten erlaubte. Die zusätzliche Ig-ähnliche Domäne im InlB321 resultierte im Vergleich zum InlB241 in einer Erhöhung der freien Enthalpie der Entfaltung (8.8 kcal/mol im Vergleich zu 4.7 kcal/mol). Diese …
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Design of a Shape Memory Alloy Coil Actuated Robotic Finger with Compliant Joint Antagonists
… The SMA coils are designed using a static two-state model, based on the required actuation stroke at discrete force-displacement coordinates. SMA coils are manufactured and characterized to obtain the actuator profiles for the SMA. The experimental profiles for the actuator and structure …
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Folding Studies on Mutants of Chymotrypsin Inhibitor 2
… (CI2) have been studied. All mutants adhere to a two-state model for protein folding , and are destabilised relative to wild-type. Mutation of N-cap residue S31 to Ala or Gly destabilises CI2 by nearly 1 kcal mol-1, with respect to both wild-type and the double mutant EA33EA34. Mutation of E33 or …
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Motion-reversal in colloidal walkers
… the dynamic and equilibrium structures are modeled using a Bell model and measured using Monte Carlo-type update steps. It is shown that the walking motion can be characterized as two different regimes corresponding to an increase of the rotating arm from half to all of the chain-length as …
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Biochemical and Biophysical Characterization of the Allosteric Equilibrium of the Wiskott-Aldrich Syndrome Protein
… equilibrium between an uninhibited, inactive state and an active state. Relief of autoinhibition then requires that covalent modification or binding events shift the equilibrium to favor the active state. However, the structural, biochemical, and biophysical properties of many autoinhibited …
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Synchronization and Signal Enhancement in Nonlinear and Stochastic Systems
… frequencies these systems are no longer well modeled by Kirchhoffs laws, and new dynamical equations are derived directly from Maxwells equations. From these equations we derive a reduced set of averaged equations which greatly simplify the analysis of high frequency arrays. The averaged …
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Ultrafast excited state reaction dynamics in light-driven unidirectional rotary molecular motors and fluorescent protein chromophores
Excited state dynamics on an ultrafast timescale can provide insight into primary events in photochemical and photobiological processes. In this work, excited state dynamics of two important systems are characterized: unidirectional molecular rotary motors and HBDI derivatives (synthetic …
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Faltungseigenschaften des extrazellulären Proteins Internalin J und seine Cysteinleiter
… Diese Topologie macht repeat-Proteine zu idealen Modellproteinen, um die stabilitätsrelevanten Wechselwirkungen zu separieren und zuzuordnen. In der vorliegenden Arbeit wurde die Faltung und Entfaltung von InlJ umfassend charakterisiert und die Relevanz der Cysteine näher beleuchtet. Die …
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Modulation orthostérique et allostérique du PAFR par des molécules synthétiques
… couplés aux protéines G, les GPCRs. Le"two state model" assume que les GPCRs existent dans un état d'équilibre entre un état inactif (R) et un état actif (R*). L'isomérisation de R vers R* peut arriver de façon spontanée, c'est à dire indépendamment de la liaison d'un agoniste. Dans ces …
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Synthesis, non-linear optical and electrochemical properties of novel organoimido polyoxometalate derivatives
… leading to high NLO activity based on the two state model.
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Protein folding and diffusion: from in vitro to live cells
… generation of experiments, as well as in-cell modeling. We discussed some specific examples of how the cell modulates protein folding kinetics and diffusion. We also provide a hidden Markov model for studying the folding and binding of the single- and few-molecule experiment. In the first …
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Decoding the Fabric of Life: Nanopore Sensing of Nucleic Acid Structure from the Primary to the Quaternary
… I leveraged RNA/DNA nanotechnology and solid-state nanopore sensing to detect RNA structural modifications and to characterise the kinetics of nanostructure formation. Additionally, I aimed to directly identify triplexes solely using nanopore sensing. The group’s RNA Single-nucleotide …
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Probing static disorder in protein unfolding and chemical reactions by single-molecule force spectroscopy
… that governs the reaction. The widely accepted two-state kinetic model for protein unfolding describes that the protein unfolds by crossing over a single energy barrier, with the implicit assumption of a single transition state and a well-defined activation energy barrier. Based on this …
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TRANSMISSION LINE RELIABILITY MODELING INCORPORATING EXTREME ADVERSE WEATHER CONSIDERATIONS
… illustrates the development of a reliability model for two redundant transmission lines, which incorporates normal and bad weather conditions. The two indices of system average failure rate and system average outage duration are evaluated for this model using a Markov approach, an approximate …
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PhoQ: Structural and Mechanistic Investigations into an Important Bacterial Sensor Kinase
Two-component systems represent the dominant mechanism for cellular signal transduction in prokaryotes. One particular system, PhoQ, has been the focus of our interest because it is the switch that controls virulence in Salmonella and other pathogenic gram negative bacteria. Certain domains of PhoQ …
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Dynamics and mechanics of associating polymer networks
… relationships of associating polymer networks. This thesis first examines diffusion of various types of associating polymers in the gel state through a combination of experiment and theory. By using forced Rayleigh scattering (FRS), phenomenological super-diffusion is revealed as a …
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Barrier Heights and Diffusion Coefficients in Protein Folding
… of single-domain proteins is that they are two-state. In other words, it is seemingly sufficient to invoke just two thermodynamic macrostates - folded and unfolded - to explain the experimental data with a transition-state like picture. Unfortunately, a chemical two-state model and the …
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A Critical Evaluation of Screening versus Sample-by-Sample Analysis of Protein Aggregation using a Variety of Chromatographic and Spectroscopic Techniques
… aggregation by characterising the aggregation of two model proteins, lysozyme and bovine serum albumin, using a variety of chemical and biophysical techniques. High-throughput assays are commonly utilised for screening protein formulations. Two high-throughput assays were critically examined to …
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Two-state systems with non-Markovian thermal and squeezed quantum noise inputs
The effect of quantum noise on the two-state system is studied in the context of (i) coherent quantum tunnelling in a double-well system subject to low temperature quantum noise, and (ii) the two-level atom subject to finite bandwidth squeezed noise. Our approach to these problems is based on the …
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Population dynamics of bacterial persistence
The life of microorganisms is characterized by two main tasks, rapid growth under conditions permitting growth and survival under stressful conditions. The environments, in which microorganisms dwell, vary in space and time. The microorganisms innovate diverse strategies to readily adapt to the …
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