Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"two-photon fluorescence microscopy"”.
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Spatial correlation of lipid phase fluctuations in model membranes studied with two-photon fluorescence microscopy
… of model lipid membranes was studied using Two-photon excitation fluorescence microscopy. Lipid membranes of different composition were reconstituted in Giant Unilamellar Vesicles (GUVs) and labeled with the environment sensitive dye LAURDAN whose spectral properties are sensitive to the …
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Investigating the mechanotransduction by two-photon fluorescence microscopy measurement of intracellular free energy of binding
… of interest, GFP-Paxillin and FAT-mCherry, using two-photon excitation fluorescence microscopy and the utility of it as a measure of protein conformational change. The hypothesis tested is that force-induced changes in protein conformation alter inter-protein binding affinity. A comprehensive …
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Advances in Two-Photon Fluorescence Microscopy for High-Resolution Anatomical and Functional Imaging of Cell Populations in the Intact Brain
Die Zwei-Photonen Mikroskopie hat die hochauflösende Untersuchung einzelner Zellen im Gehirn anästhesierter Tiere ermöglicht. Die vorliegende Arbeit beschreibt die Weiterentwicklung dieser Technik in Richtung Zellpopulationsstudien im Neokortex von freilaufenden Nagetieren. Insbesondere wurden zwei …
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Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy
Two-photon fluorescence microscopy has proven to be an useful technique for imaging biological systems. Good spatial resolution, reduced photobleaching, and efficient rejection of excitation wavelength from emitted fluorescence are the main characteristics of this technique. As part of this work, …
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Massively multiplexed imaging probes for comprehensive single-cell analysis
Optical microscopy techniques are widely used to study cellular physiology in their native tissue environments. In particular, the use of fluorescent probes to tag different cell populations, subcellular compartments, specific proteins and nucleotide sequences has enabled examination of cellular …
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Applications of pump-probe and two-photon techniques in time-resolved fluorescence miscroscopy and spectroscopy
… and improved spatial resolution similar in two-photon excitation microscopy. Using this technique, spectroscopic data measuring the lifetime and rotational correlation time of aqueous rhodamine B are presented. Furthermore, timeresolved, microscopic images deomonstrate this technique's …
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Lithograph regulation of cellular mechanical properties c by Tsu-Te Judith Su.
A magnetic trap in combination with two-photon fluorescence microscopy was used to determine the cytoskeletal stiffness and three-dimensional (3D) cytoskeletal structure of NIH 3T3 fibroblast cells plated on micropatterned substrates. Microcontact printing of self-assembled monolayers (SAMs) of …
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Optical characterisation of the interaction between calcium-silicate based dental restorative materials and dentine
… as tandem scanning and laser scanning confocal microscopy, which were both used with cement labelling and micropermeability tests to evaluate the interfacial morphology and microscopic appearance along with scanning electron microscopy. Additionally, two-photon fluorescence microscopy was …
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Photophysics of Organic Probes and their Applications in Bioimaging & Photodynamic Therapy
… the phenomenon of luminescence (divided into fluorescence and phosphorescence) has received great attention in the field of biological science. This quest has motivated scientists for a variety of applications, including fluorescence imaging. Fluorescence microscopy techniques that provide …