Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 48 for “"two-photon excitation"”.
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Nanoshells in vivo imaging using two-photon excitation microscopy
… in small animal blood vessel imaging. Two types of gold-silica nanoshells excitable by NIR lasers are investigated: Type 1 nanoshells can be excited with a sub-mum NW laser, whereas Type 2 nanoshells can be excited with a NW laser in the micrometer range. Using NIR microscopy as an …
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Detection of Proteins by Two-Photon Excitation of Native Fluorescence
… represents the development of laser-induced two-photon fluorescence detection on a microchip separation device. This detection is based on excitation of native fluorescence of aromatic amino acids by simultaneous absorption of two photons. First, a compact two-photon prototype detector was …
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Coherent two photon excitation within an extended cloud of Rubidium 85 for the purposes of atomic interferometry and cooling
… on the continuous absorption and re-emission of photons in a closed optical cycle. This requirement is difficult to achieve when there are many allowed decay paths for the excited atom, making Doppler cooling only feasible for a handful atoms with simple energy level structures. More exotic …
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THE SYNTHESIS, CHARACTERIZATION, AND UPCONVERTING PROPERTIES OF ERBIUM DOPED; YTTERBIUM, ERBIUM CO-DOPED YTTRIUM OXYSULFIDE PHOSPHORS UNDER 808, 980, AND 1560 nm EXCITATION
… were observed under 808, 980, and 1560 nm excitation. The upconverting mechanisms excited at 808, 980, and 1560 nm have been investigated in detail. The power dependent behavior of the upconverted emission intensities for singly doped erbium phosphors excited at 808 and 980 nm indicated a …
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Ultrafast optical pulse manipulation in three dimensional-resolved microscope imaging and microfabrication
… aspects of utilizing ultrafast light pulses in two-photon excitation microscopy. First, optical fibers are routinely used in many optical instruments but their use in two-photon microscopy is very limited. As ultrafast light pulses propagate through conventional fiber optics, light pulses are …
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Supramolecular quantum dot-porphyrin assemblies for biological oxygen sensing
… oxygen quantification. The QD serves as a two-photon antenna to enable sensing under two-photon excitation. Multiphoton imaging is a powerful technique that is nondestructive to tissue and provides high-resolution images of live tissue at depths of several hundred microns with submicron …
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Extending imaging depth of multiphoton microscopy
Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the …
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Advancing fluorescence lifetime microscopy for quantitative bioimaging
… neurodegeneration and stem cell differentiation. Two FLIM technologies enabling faster FLIM acquisition were developed and tested for high-throughput screening of animal models of neurodegenerative diseases. Each technical implementation was characterised and compared in terms of measurement …
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Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion
… This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of …
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Characterization of Organic and Inorganic Optoelectronic Semiconductor Devices Using Advanced Spectroscopic Methods
… lasers (VCSEL). Experiments include single photon and two-photon excitation with lasers, with subsequent measurements of the absorption and photoluminescence, as well as photocurrent measurements using tungsten and xenon lamps, measuring the direct current and the alternating current of the …
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Synthesis, Characterizations, And Evaluation Of New Reactive Two-photon Absorbing Dyes For Two-photon Excited Fluorescence Imaging Applications
… in the emergence of a novel area termed 'biophotonics'. The integrative and interdisciplinary nature of biophotonics cuts across virtually all disciplines, extending the frontiers of basic cellular, molecular, and biology research through the clinical and pharmaceutical industries. This holds …
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Local Viscosity and Environment on the Nanometer Scale
New optical setup for two photon excitation time-resolved fluorescence anisotropy and lifetime measurements was built and used to understand the local environment and viscosity. The rotational correlation time constants by the fluorescence anisotropy provide the information on the hydrodynamic …
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Fluorescence Detectors for Proteins and Toxic Heavy Metals
… described. The detection technique was based on two-photon excitation intrinsic protein fluorescence using a visible 532 nm diode-pumped nano laser as the excitation source. Proteins that exhibit intrinsic fluorescence must contain at least one tryptophan, tyrosine, or phenylalanine residue in …
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The heterogeneous biochemical modification of porous collagen scaffolds for tissue engineering applications
… towards a vasculogenic lineage. The use of a two photon excitation for BP patterning was proven affective for surface patterning but ineffective for patterning past a couple hundreds of microns. Heterogenous scaffold patterning in all three dimensions was achieved by collagen slurry layering …
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Interactions in an ultracold gas of Rydberg atoms
… and a suppression of resonant Rydberg excitation. <br>The latter effect can be interpreted as the onset of a local dipole <br>blockade and can be exploited to perform quantum information processing <br>with mesoscopic ensembles. The dominating interaction between a <br>pair of Rydberg …
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Spatial correlation of lipid phase fluctuations in model membranes studied with two-photon fluorescence microscopy
… of model lipid membranes was studied using Two-photon excitation fluorescence microscopy. Lipid membranes of different composition were reconstituted in Giant Unilamellar Vesicles (GUVs) and labeled with the environment sensitive dye LAURDAN whose spectral properties are sensitive to the …
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Dual-Colour STED-microscopy on the Nanoscale
… been traced in a comprehensive study on one- and two-photon excitation to crucially reduce photobleaching in general thus leading to a major signal increase per excitation pulse. This facilitated a successful combination of this illumination strategy with STED-microscopy making a 10-fold increase …
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Theory Advances in High-Precision Two-Photon Spectroscopy
High-precision two-photon spectroscopy of hydrogen and hydrogenlike systems constitutes an exceptional tool for experimental tests of fundamental theories. In the analysis of these experiments, theory also plays a vital role in the modeling of time-resolved spectra, the calculation of precise …
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Protein Dynamics: Studies of Adenylate Kinase Mutants From Escherichia Coli and Characterization of Adenylate Kinase Isoforms From Murine Cells. Applications of Fluorescence Spectroscopy and Microscopy
… the thesis is focused on the characterization of two adenylate kinase isoforms (AK1 & AK1beta) from murine cells using a series of fluorescence microscopic techniques (two-photon excitation fluorescence imaging, fluorescence correlation spectroscopy (FCS), fluorescence lifetime imaging (FLIM)). In …
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Tissue spectroscopic characterization based on fluorescence, second harmonic generation, and reflected light
… and understand tissue structure and function: two-photon spectral resolved imaging, tri-modal imaging, and interferometric second harmonic imaging. First, two-photon spectral resolved microscopy utilizes the 3D localization ability of two-photon excitation to extract spectroscopic information …
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