Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 35 for “"two-phase system"”.
-
The Study of 3D Micro-fluid Printing in Aqueous Two-phase System
<p>Aqueous two-phase system (ATPS) is a technology that allows phase separation in an aqueous environment. This technology can be used for the separation, extraction and purification of a variety of biomolecules and thus gained great attention in industry and academia. This thesis is a combination …
-
Experimental investigation of interfacial tension in two-phase system involving methane and water
… hydrocarbon fluids involve dealing with the multiphase system. Viscosity and interfacial tension (IFT) are among the fluid properties that affect fluid behaviour. These properties have significant effects on fluid flow characteristics, and thus their importance in oil and gas production and …
-
Fundamental phenomenological description and experimental optimization of gel stabilized biocatalysts in a two-phase system
… consequence of the acidification of the aqueous phase lead to the evolution of the calcium-alginate network to an alginic acid one with a size reduction of the spheres and a release of 10-15 % of water (w/w) according to the calcium-alginate preparation. The control of the pH can be only achieved …
-
Optimization of sequential purification of beta-glucosidase from tricoderma reesei in aqueous two-phase system
… chitosan followed by separation with an aqueous two-phase system (ATPS), was implemented for the purification of beta-glucosidase from the culture filtrate of T. reesei. The cultivation medium (nutrient) was optimized for the production of betaglucosidase from T. reesei cell culture. Treatment of …
-
Experimental study of rapid pressure transients in a two-fluid, two-phase system and comparison to RELAP5
The six-equation two-fluid two-phase model is a prevalent tool used to estimate the behavior of nuclear thermal hydraulic systems. This model requires use of closure relations, which have been developed over decades to provide greater accuracy and reliability to the simulations that utilize this …
-
Cloning, expression, charakterization and immobilization of carbonyl reductase from Candida parapsilosis
… type strain of C.parapsilosis. Amplification of two alcohol dehydrogenases from the “CPCR” type strain of C.parapsilosis showed that at least two ADHs were present in the same C.parapsilosis strain (DSMZ 70125 strain) though it was not known which one of the two was the “Real CPCR”. With …
-
Monoclonal antibody (mAb) purification by counter current chromatography (CCC)
… (mAb) from mammalian cell culture, using aqueous two-phase systems (ATPS) of inorganic salts and polymer. A polyethylene glycol (PEG) and sodium citrate system was found to be the most appropriate by robotic phase system selection. The search for an economical alternative to protein A HPLC is a …
-
Charakterisierung und Anwendung eines gelstabilisierten Zweiphasensystems für die asymmetrische Synthese neuartiger Benzoine mit immobilisierter Benzaldehydlyase
… exclusively described for aqueous monophasic systems. In such systems the number of convertible hydrophobic substrates is limited, the turn over number is partly unsatisfactory and additives which are commonly used for the improvement of the substrate solubility have a negative influence on …
-
Synthetic routes to monodisperse gold nanoparticles stabilized by different-length alkanethiols
… stabilized by various alkanethiols. The first two synthesis methods were based on a two-phase system employing first tetraoctylammonium bromide (TOAB) as a phase transfer catalyst and then didodecyldimethylammonium bromide (DDAB). Though these methods approximated what could be considered …
-
Oligomerisierung von 1,3-Butadien und Hydroformylierung höherer Olefine zu Oligoaldehyden
… Afterwards the homogeneous catalyst system is extracted by carbon dioxide from the product. In the third part of the work a system is introduced which allows the selective hydroformylation of certain olefines out of olefin mixtures with different chain lenghts. The problem is solved …
-
A study of flow-induced crystallization in two-phase polymer melts
… in polymer melts were investigated for the two-phase system of high molecular weight polyethylene suspended in a linear low density polyethylene carrier phase. Shearing flow was produced by a counter-rotating couette device and elongational flow was produced by means of a four roller mill …
-
Entwicklung und Untersuchung eines Verfahrens zur integrierten Aufreinigung von Plasmid DNA mittels wässriger Zweiphasenextraktion
… of crude impurities and cell debris using two phase extraction. In this study, a systematic screening led to an optimal system comprising 15% PEG 800 and 20% potassium phosphate. This system resulted in a plasmid DNA recovery of about 90 % plasmid in the bottom phase, while most of …
-
Fabrication of porous ceramics and composites by a novel freeze casting process
… for potential load-bearing applications. In a two-phase system (Al\(_2\)O\(_3\)-ZrO\(_2\)), the choice of particle size of the ceramic powder was of great significance, which can lead to engulfment and phase segregation. BaTiO\(_3\) and Lead zirconate titanate (PZT) were employed to demonstrate …
-
Microfluidic bubble logic
… material manipulation in an all-fluidic two-phase system. This removes the distinction between materials and mechanisms to control them, bringing the programmability of the digital world into the physical world - with a wide range of promising applications in biotechnology, highthroughput …
-
Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction
… tobacco with high yield and purity. Aqueous two-phase extraction (ATPE) is a powerful technique for separation and purification of proteins, and has the potential to replace an expensive chromatography step for the initial purification of recombinant proteins. ATPE enables high levels of …
-
Charakterisierung und Optimierung gel-stabilisierter Zwei-Phasen-Systeme zur stereospezifischen Reduktion hydrophober Verbindungen mit der Carbonyl-Reduktase aus Candida parapsilosis
… offers a convenient alternative to the aqueous system. Use of a gel-stabilized two-phase-system could avoid an inactivation of the sensitive biocatalysts by the solvent. The biocatalysts are protected in the hydrogel from the detrimental effects of the solvent which serves as a reservoir for the …
-
Green grass: developing grass for sustainable gaseous biofuel
… After extensive analysis of the literature, two different digester systems were designed, fabricated, commissioned and operated. The first system was a two stage wet continuous system commonly referred to as a Continuously Stirred Tank Reactor (CSTR). The second was a two stage, two phase …
-
Asymmetrische Synthese von cyclischen allylischen S-tert-Butylsulfonen : Pd-katalysierte kinetische Racematspaltung racemischer Allylcarbonate und zur Struktur chiraler, cyclischer allylischer Alpha-tert-Butylsulfonyl-Carbanionen
… carbonates and lithium-tert-butylsulfinate in a two phase system composed of dichlormethane and water at 0 °C containing THAB as a phase transfer catalyst and in presence of Pd(dibenzylidenacetone)3·CHCl3 as precatalyst and Pd(0)source and the chiral catalyst. Cyclopentenyl-tert-butylsulfone, …
-
Gold Nanomolecules : Developing Synthetic Protocols, Characterization And Investigation Of Ligand Effects On Structure And Properties
… of nanomolecules yields stable products in a two-phase system which can be dried and re-dispersed without affecting the stability. But the protocol has a major drawback of producing a polydisperse mixture of different sizes of nanomolecules. A major portion of my dissertation focuses on …
Page 1 of 2