Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 66 for “"two-hybrid screen"”.
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Analysis and confirmation of the results of a yeast two-hybrid screen carried out to identify proteins that interact with drosophila XRCC2
… that partake in a variety of DNA repair. Two of the most common types of damage are double-strand breaks (DSBs) and interstrand crosslinks. A single DSB or crosslink can potentially kill a cell if it is not repaired~ In human and other vertebrate cells, DSBs are repaired by two different …
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FUNCTIONAL CHARACTERIZATION OF DENND5A/RAB6IP1 IN EARLY EMBRYONIC DEVELOPMENT
… involves in intra-Golgi transport. In a yeast two hybrid screen by Bruno Goud group in 1995, they used Rab6 as bait and found several interacting cDNA clones including Rab6 interacting protein 1 or Dennd5a. Several Dennd5a interaction partners have been discovered, Rab6, Rab11 and Sorting Nexin …
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Regulation der BOB.1/OBF.1-Expression und der BOB.1/OBF.1-Proteinstabilität
… des BOB.1/OBF.1-Proteins. In einem "yeast-two hybrid screen" mit der amino-terminalen Domäne von BOB.1/OBF.1 als "bait" (Köder) konnten die beiden Proteine SIAH1 und SIAH2 als Interaktionspartner identifiziert werden. Eine beschriebene Funktion von SIAH1 und SIAH2 liegt in der Regulation …
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Funktionelle Charakterisierung der humanen Proteinkinase Aurora-C in Krebszellen
… der Kinase Aurora-C durch einen Two-Hybrid-Screen in der Hefe Saccharomyces cerevisiae. Unter anderem wurden drei Proteine (Calpain-4, Epiplakin, Interleukin 15) identifiziert, die wie Aurora-C eine Veränderung der Zellmorphologie bewirken können. Im Rahmen dieser Arbeit wurde von …
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Die Rolle der Serin/Threonin Kinase Cot in Proliferation, Differenzierung und Apoptose
… von NF-kB, als Cot-Interaktionspartner in einem Two-Hybrid Screen gefunden werden. Die Tatsache, daß Cot mit IKKa und IkBa, beides NF-kB-Regulatoren, copräzipitiert, deutet ebenfalls auf einen direkten Mechanismus der Cot-abhängigen NF-kB-Aktivierung hin. Dieser Prozeß wird hauptsächlich durch …
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The Role of Two Homologous E3 Ligases In Muscle Physiology
… often remains unknown. FBXL3 and FBXL21 are two homologous E3 ligases that have previously been reported to dictate circadian periodicity, with FBXL3 being the dominant E3 ligase and FBXL21 playing a regulatory role. A recent Yeast Two-Hybrid screen revealed a new shared target of FBXL3 and …
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Identification and characterization of signal components in early ABA signaling in Arabidopsis
… the ABA signaling pathway, we performed a yeast two-hybrid screen for PYLs and SnRK2s interactors. We identified two proteins that interact with PYLs and SnRK2s: Ty pe One Protein Phosphatase 1 (TOPP1) and its regulatory protein At Inhibitor 2 (AtI-2). We show that TOPP1 and AtI-2 inhibit SnRK2.6 …
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Elucidation of Snm1B Function
… performs these functions I performed a yeast two-hybrid screen to identify proteins binding to the C-terminus of Snm1B. From this screen I identified PSF2 (GINS2), a member of the tetrameric protein complex GINS, to bind Snm1B. The GINS complex is required for replication initiation and …
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Identifizierung und funktionelle Charakterisierung des Spleißfaktors SRp30c als Interaktionspartner des Y-box-Proteins-1 (YB-1)
… an interacting protein of YB-1 by performing a two-hybrid screen against a human mesangial cell cDNA library. Co-immunoprecipitation studies confirm a direct interaction of tagged proteins YB-1 and SRp30c in the absence of RNA via two independent protein domains of YB-1. A high affinity …
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Daple Orients and Patterns Sensory Hair Bundles in the Inner Ear
… of diverse pathways. In the inner ear, at least two systems regulate the planar polarity of the hair bundle. The core planar cell polarity (PCP) proteins coordinate the orientations of hair cells across the epithelial plane. The cell-intrinsic patterning of each hair bundle is implemented …
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THE ROLE OF TIGHT JUNCTION PROTEIN ZO-2 IN THE NUCLEUS AND ITS LINK TO TRANSCRIPTION FACTORS OF THE SLUG/SNAIL FAMILY
… identify proteins interacting with ZO-2, a yeast two-hybrid screen was carried out and a transcription factor was isolated as a novel interacting partner. This transcription factor was shown to possess functional nuclear localization signals, nuclear export signal and zinc fingers that are …
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With No Lysine 1 (WNK1): A Potential Regulator of The Lysosomal Degradation Pathway
… I performed a WNK1 kinase activation screen and a WNK1 yeast-two-hybrid screen. I have shown that WNK1 kinase activity increases in response to osmotic stress, which may imply its kinase activity is important for regulating ion homeostasis in response to a change in cell volume. I have …
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An investigation into the role of the ubiquitin-proteasome system in plant defence
… the interactor of UBP12 by employing a yeast two hybrid screen. The final objective was to analyse the functional significance of the mutant forms of ubiquitin during ubiquitination of target protein. Added to the above-mentioned objectives, this study also details the production of plant …
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Characterizing the Role of Spegless, a Novel Inner Nuclear Membrane Protein required for spermatogenesis and oogenesis in Drosophila melanogaster
… processes is not fully understood. A yeast two-hybrid screen previously identified proteins that can bind the cytoplasmic domain of Fat. Using genome-wide RNAi libraries of transgenic flies, I knocked down the function of each gene identified through the screen in the Drosophila eye. Through …
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Identification of a Novel ERK 1/2-Interacting A-Kinase Anchoring Protein
… was found to interact with ERK2 through a yeast two-hybrid screen designed to identify interactors that have a higher affinity for the phosphorylated, active form of ERK2. Confirming this finding, the human homolog long form, RSP3H, co-immunoprecipitates with ERK1/2 in HEK293 cells. Human RSP3, …
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Study of Ochratoxin A biosynthesis by Aspergillus ochraceus and incidence of ochratoxigenic fungi on Wine Grapes from Southern Illinois
… an abnormal mitochondrial phenotype. A yeast, two-hybrid screen revealed that Aomdv1 interacts with proteins involved in regulating mitochondrial functions necessary for mycotoxin production. To further understand the role of Aomdv1 in OTA production in <A. ochraceus>, we used a cDNA-AFLP …
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Molekulare Mechanismen der transkriptionellen Aktivierung durch die Transkriptionsfaktoren c-Jun und v-Jun
… Aktivierung involviert sind, mit Hilfe des ?two-hybrid screens? isoliert werden. Als Interaktionspartner wurden in einer HeLa cDNA-Bank PAG und NKEF-B identifiziert. Beide Proteine weisen ein hohes Maß an Homologie auf und kontaktieren spezifisch die Transaktivierungsdomäne von v-Jun. Die …
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The dynamics of molecular components that regulate aphid-plant interactions
… via interaction with other RLKs. I identified two RLKs which may detect aphid elicitors and provide specificity to aphid detection. Successful aphid colonization of plants is thought to involve the suppression of PTI and ETI via effectors, leading to effector-triggered susceptibility (ETS). I …
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Investigating Potential Multi-Protein Complexes Involving Emx2
… Previous researchers in our lab have identified two Emx2-interacting proteins, Cnot6l and QkI (which exists in three forms) that interact with each other. These interactions were initially identified by a yeast two-hybrid screen and then confirmed by pull-down assays. The goal of this project is …
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