Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"two-electrode voltage clamp"”.
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Two-Electrode Voltage Clamp (TEVC) Based Scanning Ion Conductance Microscopy: New Methodology and Validation
… microscopy, confocal microscopy, and patch clamping. A key feature of SICM is its non-invasive scanning ability. The ability to scan samples without coming making contact allows for more robust research in biology. Current SICM techniques call for a one electrode probe, but in this research …
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The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… on CFTR function via Ussing chamber analysis, two-electrode voltage clamp analysis, preliminary <em>in vivo</em>animal studies, and protein phosphorylation. The HEK-293 Flag-CFTR cells used in this study allows for a high degree of protein purification in quantities sufficient for mass …
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Investigation of an unusual GABA-A receptor: studies on the promiscuous ε [epsilon] subunit
… examined in Xenopus laevis oocytes using the two-electrode voltage-clamp technique. The results obtained confirm that the ε subunit confers unusual properties on the GABAA receptor.
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An examination of interacting residues in the GABA-gated ion channel UNC-49 within the parasitic nematode Haemonchus contortus
… these positions were introduced and analyzed by two-electrode voltage clamp electrophysiology. It was found that both residues are important for receptor function, but modifications to the E183 residue yielded a greater negative impact. It was also found that K181 and E183 are energetically …
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Isolation and partial characterization of three acetylcholine-gated chloride channels in Haemonchus contortus
… Further pharmacological analysis of ACC-2 via two-electrode voltage-clamp electrophysiology demonstrated activity of several agonists including carbachol, which elicited a similar response as acetylcholine. Furthermore, in silico protein modelling and agonist docking of all three channels has …
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Molecular Cloning and Functional Characterization of a Turkey Intestinal Peptide Transporter (tPepT1), and Developmental Regulation of PepT1 Expression in Turkey and Broiler Embryos
… from rat, sheep, rabbit, and human. Using a two-electrode voltage-clamp technique in Xenopus oocytes expressing tPepT1, Gly-Sar transport was pH dependent, but independent of Na+ and K+. For the dipeptides Gly-Sar and Met-Met, the evoked inward currents indicated that the transporter was …
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Molecular Cloning, In Vitro Expression, and Functional Characterization of an Ovine Gastrointestinal Peptide Transporter (oPepT1)
… by expressing oPepT1 in Xenopus oocytes using a two-electrode voltage-clamp technique. Overall, the in vitro transport characteristics of oPepT1 expressed in oocytes were similar to those of PepT1 from other species. The transport process is electrogenic and pH-dependent, but independent of Na+, …
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Structure and function of potassium and calcium channels
… or electrophysiological experiments using two electrode voltage clamp. For protein expression studies,DNA was expressed in BL21 or COS-7 cells and purified using glutathione or an ID4 affinity column. Firstly, the roles of the N- and C- terminal domains in the activation kinetics of rat and …
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Voltage-Gated Sodium Channel Activity in Mouse Skeletal Muscle Fibers: Normal Gating and Defects Associated with Periodic Paralysis Mutants
… in mature skeletal muscle. We optimized a two-electrode voltage clamp protocol to improve the fidelity of Na+ current recording from acutely dissociated intact muscle fibers. Computer simulation, incorporating measured capacitance and ionic current densities, was used to confirm sufficient …
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Functional characterisation of aphid, Myzus persicae, nicotinic acetylcholine receptors and identification of a novel biogenic amine-gated ion channel from the honeybee, Apis mellifera
… pharmacological properties of these receptors by two-electrode voltage clamp electrophysiology. Myzus persicae, the peach potato aphid, is a major global pest of multiple crop species. The nAChR subunits and several protein chaperones from Myzus persicae have been cloned. It was difficult to …
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Characterization of L-type Calcium Channel Binding-Site of a new class of Calcium modulators by a Multidisciplinary approach
… β3 subunits) in Xenopus Leavis oocytes using a two-electrode voltage clamp technique. Diltiazem is a benzothiazepine Ca2+ channel blocker used clinically for its antihypertensive and antiarrhythmic effects. Previous radioligand binding assays revealed a complex interaction with the …
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The Lipid-Facing M4 Helix of α4β2 nACh and 5-HT3A Receptors
… a membrane potential-sensitive fluorescent dye, two-electrode voltage clamp and manual patch-clamp for functional characterisation of mutant receptors in HEK293 cells and Xenopus oocytes, and radioligand binding and immunofluorescence to assess ligand binding and receptor expression. I show that …
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Computational modelling of the glycinergic synapse
… IPSC. In addition, electrophysiological two electrode voltage clamp (TEVC) techniques were used in order to characterize the effects of a newly identied NKH-causing mutation at position Ser 407 on GlyT1 expressed in Xenopus laevis oocytes. The mutation lead to the inhibition of transport …
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Functional Characterization of the Mammalian TRPV4 Channel: Yeast Screen Reveals Gain-of-Function Mutations
… substitution in the C-terminal tail (H787Y), and two truncations of the C-terminal tail (N789Δ and Q790Δ). Each of these mutations was assayed, in both the variant V4-K70E/M605T and the wild type TRPV4 background, for effect on rescue of trk1Δ trk2Δ yeast low-potassium growth, as well as …
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Substrate specificity and functional characterization of sodium/dicarboxylate cotransporters
… The studies in this dissertation focused on two aspects of the NaDCs: structure-function studies of two low-affinity transporters, mouse (m) and rabbit (rb) NaDC1, and functional characterization of a high-affinity NaDC transporter from Xenopus laevis, xNaDC3. \r\n\r\nAlthough sharing strong …
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Functional and behavioural characterisation of C. elegans acid-sensing DEG/ENaCs
… oocytes. These could be further clustered into two groups based on their response to neutral and low pH in vitro: One group that is inhibited by increasing concentrations of protons (ACD-5 and DEL-4, as well as the previously identified ACD-1 (Wang et al., 2008)) and the other one that can be …