Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"two-dimensional gel electrophoresis"”.
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An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram
Although there have been two-dimensional electrophoretic studies on fetal brain tissue (for instance, Yoshida and Takahashi, 1980), the emphasis in most of this work has been on developmental changes in protein expression, and not on the effects that drugs have on fetal brain protein complement. …
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Cytological and Biochemical Studies of Male Sterility in Two Species of Drosophila
… melanogaster mutant ms(3)K81 were examined by two-dimensional gel electrophoresis, isoelectric focusing and Western blotting to determine the biochemical basis for sterility. One protein, KRP60, was consistently reduced in sterile males. This protein may play a role in the normal function of …
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Biochemical and genetic characterization of U1 and U3snRNAs from Schizosaccharomyces pombe
… pombe. In vivo labeling followed by two dimensional gel electrophoresis of S. pombe RNA demonstrates the presence of four prominent species in the size range of interest (100-300 nucleotides). Further analysis of these has shown that they represent U1, U2, U3, and 7SL homologs.
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Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry
… and harvested after 7 days exposure to BrdU. Two-dimensional gel electrophoresis was used to investigate BrdU specific changes in the proteome of DLKP BrdU treated and control cells. Cy3-labeled DLKP control were combined with Cy-5 labeled BrdU DLKP treated proteins and separated on the same …
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CANNABINOIDS DELTA-9-TETRAHYDROCANNABINOL (THC) AND CP55,940 ABLATE SPECIFIC CHEMOKINE AND CYTOKINE INFLAMMATORY RESPONSE IN BV-2 MOUSE MICROGLIAL CELLS ACTIVATED WITH HIV-1 PRO-INFLAMMATORY PROTEIN TAT
… at the level of the proteome as tested by two-dimensional gel electrophoresis . In contrast, these cannabinoids ablated the inflammatory response caused by the HIV protein Tat at the level of the secretome and at the level of gene expression. These collective observations suggest that …
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Characterization of a novel aggregate-gland-derived spider silk protein in Latrodectus Hesperus : from sequence to propsoed function
… modifications were also analyzed by two-dimensional gel electrophoresis (2DE) and MS/MS analysis. AgSFl was localized in the web scaffolding junctions and our data supports a role as an adhesive silk protein that serves as a center for connecting scaffolding fibers that functions to …
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Investigation of the Gracilaria gracilis proteome response to nitrogen limitation
… on the west coast of South Africa, is one of two Gracilaria species used in the production of hydrocolloid agar. In the past, commercial quantities of G. gracilis were harvested from Saldanha Bay until population collapses of the natural resource necessitated the need for research into …
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Changes in proteins during the cell cycle of Physarum polycephalum
… total plasmodial proteins were resolved by two-dimensional gel electrophoresis and visualized by a sensitive silver stain. The results indicate that most proteins do not change in concentration during the cell cycle. The one protein which changed consistently on the gels had a 32,000 …
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Understanding adaptive regulation of skeletal muscle physiology in the Weddell seal: a proteomics approach
… swimming muscle (M. longissimus dorsi) of two distinct age classes, pups (3-5 weeks/nondivers) and adults (7+ years/expert divers), were visualized using two dimensional gel electrophoresis (2DE), quantified, and identified. This study validated the use of cross-species analysis, which was …
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Characterization of Changes in Composition and Physicochemical Properties of Casein Micelles from Raw Milk to Buttermilk
… milk, and buttermilk were analyzed using one and two-dimensional gel electrophoresis technique. Experiments were performed using three different batches of UP cream, skim milk and buttermilk. Statistical analyses showed that processing the buttermilk significantly increased the surface charge …
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Exploring Metallic Flavor Perception: Analysis of Human Salivary Proteins and the Use of the Iron-Binding Protein Lactoferrin in Reducing Metallic Off-Flavors
… and metallic stimuli from four panelists by two-dimensional gel electrophoresis. A consistent pattern of regions containing major salivary components was observed. This research has shown that lactoferrin protein is a potential natural alternative to synthetic EDTA for reducing iron-induced …
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Major Sclerotial Polypeptides Of Psychrophilic Fungi: Identification, Immunological Relatedness, Localization And In Vivo Synthesis
… polypeptides have been identified by one and two-dimensional gel electrophoresis in Myriosclerotinia borealis, Coprinus psychromorbidus, Typhula idahoensis and Typhula incarnata. The number, molecular mass, relative proportions and isoelectric points of the major sclerotial polypeptides varied …
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PROTEOMIC ANALYSIS OF TWO DIFFERENT STATES OF NAEGLERIA FOWLERI
Naegleria fowleri are free-living ameboflagellates found in soil and freshwater habitats throughout the world that cause a fatal disease in humans called Primary Amoebic Meningoencephalitis (PAM). Mechanisms of host resistance or susceptibility to infection have not been fully elucidated, and …
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Analysis of the Expression Profiles of Two Isoforms of the Antifungal Protein Osmotin from Gossypium hirsutum
The expression of two cotton osmotin genes was evaluated in terms of the mRNA and protein expression patterns in response to chemical inducers such as ethylene, hydrogen peroxide, and sodium chloride. Reverse transcriptase-polymerase chain reactions (RT-PCR) indicated that osmotin mRNAs are …
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Non-Histone Nuclear Proteins : Patterns of Synthesis and Phosphorylation During the Cell Cycle, Redistribution at Times of Gene Activation and Properties of Subsets Interacting with HnRNA
… are heterogeneous ; recent estimates based on two-dimensional gel electrophoresis place the total number of different HeLa cell chromosomal proteins at over 470 (Peterson and McConkey, 1976). Many of these proteins exhibit enzymatic activities associated with nuclear functions (such as DNA …
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Resistance to protein synthesis inhibitors in Coprinus cinereus
… protein synthesis inhibitor (Sisler and Siegel, 1967) was used in a biochemical and genetical investigation of the beisidiomycete fungus, Coprinus cinereus. An optimised polyuridylic acid dependant cell-free polyphenylalanine synthesising system was developed for Coprinus cinereus, in order …
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NcCyP41, a two domain neurospora crassa cyclophilin : characterization of its peptidyl-prolyl cis/trans isomerase activity ; isolation and functional analysis of two novel NcCyP41-binding proteins
… showed no PPIase activity. Thus, NcCyP41 has two distinct domains, the PPIase domain containing the full enzymatic activity. <br> <br>NcCyP41 and its domains were used in affinity experiments to identify binding proteins. Two abundant binding proteins were identified: Hsp80 and CyPBP37. A …
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Study of the Reproducibility of Proteomics Methods and Variability of Fruit Fly Proteomes.
… of proteomic biomarker research that is largely unexplored is the comparative reproducibility of certain methods such as two-dimensional gel electrophoresis and liquid chromatography/mass spectrometry. In particular, with liquid chromatography/mass spectrometry, it is not known whether …
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Identification and isolation of growth-phase specific proteins of mycobacteria
… molecular weight stationary phase protein on one-dimensional SOS-PAGE. The protein was induced in cells entering the stationary phase of growth and not by heat shock as it was induced under both the control and the heat shock temperatures. The protein was further characterised by two-dimensional …
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COMPARATIVE PROTEOMICS STUDIES OF SOLUBLE NUCLEAR PROTEINS OF DRUG SUSCEPTIBLE AND RESISTANT HUMAN BREAST CANCER MCF-7 CELLS
… drug resistant human breast cancer MCF-7 cells. Two dimensional gel electrophoresis and stable isotope labeling by amino acids in cell culture are used to conduct the study. Protein identification is acquired by peptide fingerprinting or microsequencing. Relative quantitation of the proteins is …
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