Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"two-dimensional electrophoresis"”.
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Protein Contributions of the Male Accessory Organs to the Composition of Human Seminal Plasma as Determined by High Resolution Two-Dimensional Electrophoresis
… Plasma was evaluated using a High Resolution Two-Dimensional Electrophoresis technique adapted by this laboratory. Seminal plasma from healthy volunteers with recently proven fertility were characterized using this technique to establish "normal" protein distribution patterns in the …
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A New Method to Characterize the Translation Initiation Sites of Messenger Ribonucleic Acids
… specific cleavage reactions, in quantitative two dimensional electrophoresis/homochromatography "fingerprint" analyses greatly facilitates one's ability to determine the RNA nucleotide sequence of the translation initiation sites of mRNAs.
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Nrf2-Dependent Overexpression of Sulfiredoxin and Peroxiredoxin III in Human Lung Cancer, Especially Squamous Cell Carcinoma
… and immunoblot analysis 8 G. Two-dimensional electrophoresis 9 H. Subcellular fractionation 10 I. Immunohistochemistry 10 J. Statistical analysis 11 Ⅲ. RESULTS 12 A. Localization of Prx III and Srx in A549 cells 12 B. Oxidation of Prx III in mink lung epithelial cells 12 C. Srx …
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Analisi Molecolare del Melanoma
We have analyzed the proteomes of two human melanoma cell lines (A375 and 526), and of the human melanocytes, (FOM 78), by two-dimensional electrophoresis (2D-PAGE) and liquid chromatography¿tandem mass spectrometry (LC-MS/MS). Our comparative proteomic analysis revealed that six proteins were …
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Molecular Study of the B19 (Human) Pathogenic Parvovirus
… were performed to test the validity of two dimensional exonuclease VII analysis of overlapping transcripts. The transcripts were hybridized with a DNA probe and digested with exonuclease VII. Single bands were resolved into components of appropriate sizes by two dimensional …
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Pathogenic Potentials of Clinical MRSA Isolates from the UK and Nigeria: A Comparative Study
… factors among the isolates. With the aid of two-dimensional electrophoresis, variation was observed in the intracellular protein profiles of MRSA from the same and different lineages. Antibiogram profiling of the isolates, using antibiotic disc susceptibility and minimum inhibitory …
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Isolation and characterization of a common nuclear matrix protein and other studies
… nuclear matrix proteins isolated and analyzed by two-dimensional electrophoresis. Upon comparison, distinct nuclear matrix protein differences were observed in all of the organ sites that were investigated suggesting that the extracellular matrix, as well as growth factors and hormones, may be …
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Proteomic profiling of Nguni cattle liver tissue using gel and Gel-Free approaches: methodology development and potential applications
… employed and their outcomes compared namely, two-dimensional electrophoresis, fractionation by solution phase iso-electric focusing-reversed phase chromatography (IEF-RP), offline strong cation exchange- low pH reversed phase chromatography (SCX-RP) and offline high pH reverse phase-low pH …
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Environmental proteome profiling applied to the study of polybacterial metal resistance and adaptation
… qualitative and quantitative proteomics methods (two-dimensional electrophoresis and/or chromatography followed by tandem mass spectrometry) was used to investigate the proteome of a sequenced mixed culture, an unsequenced mixed culture, and a bacterial isolate from the original unsequenced mixed …
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Studies on the mode of action of diacetyl and carbon dioxide as inhibitors of meat spoilage bacteria
… in AOAC synthetic broth was studied using two-dimensional electrophoresis (2-DE) to determine the effect of CO2 on cellular proteins. Pure cultures were grown in AOAC broth under 0 or 40% CO2 for 7 and 12 days, respectively, at 5°C. Cellular proteins were extracted and the method of 2-DE …
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Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-culture
… Human MSC proteins were separated using two-dimensional electrophoresis and differentially expressed proteins were identified with PDQuest 8.0 and MALDI-TOF Mass Spectroscopy. The significant differences of protein expression levels were investigated using ANOVA test. It was observed that …
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Water stress protein is secreted, is subject to rapid proteolysis upon rehydration of desiccated cells, and may be glycosylated
… work. Firstly, the microheterogeneity noted on two dimensional electrophoresis gels indicated the possibility of modifications of the protein or the existence of isoforms of Wsp. This possibility was not addressed in the original study. On two-dimensional gels, several proteins were seen to …
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Proteomic approaches to environmental stress in mussel Mytilus edulis due to emerging classes of anthropogenic pollutants
… with 5-iodoacetamidofluorescein prior to one-dimensional and two-dimensional electrophoresis. This clearly revealed some similarities on a portion of the redox proteome across chemical exposures indicating where toxicity mechanism may be common and where effects are unique to a single …
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Prognostic and treatment predictive factors for radio- and chemotherapy resistance in breast cancer patients- a step towards personlized medicine
… the protein expression pattern using 2-DE (two-dimensional electrophoresis) and found several differentially expressed proteins, both when comparing distant recurrences to no recurrences and estrogen receptor positive to estrogen receptor negative tumors. Similarities of regulated genes and …
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A study of acetylcholinesterase and cholinesterase in the fetal mouse brain and a study of the effects of three teratogens, vitamin A, cyclophosphamide and sodium valproate on the fetal mouse central nervous system
There were two aims in this thesis. Firstly, to investigate cholinesterase and its isoenzymes in the fetal mouse brain, and secondly to study drug-induced fetal damage with the following objectives in mind: (i) to examine new markers for the evaluation and prediction of the teratogenic potential of …
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Monomeric Ellagitannins in Oaks and Sweetgum
… case of oaks, ellagitannin concentrations in heartwood can reach up to 10% (dry wt. basis). These secondary metabolites are not only important physiologically but also influence the economic value and quality of wood products that contain them. Efforts were made to develop and validate the methods …
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Proteome studies on leaf peroxisomes from Spinacia oleracea L. and Arabidopsis thaliana (L.) Heynh.
… Blattperoxisomen entwickelt.Die Methode der zwei-dimensionalen Gelelektrophorese wurde für lösliche Matrixproteine bezüglich der Auswahl und Konzentration chaotroper Reagenzien, Detergenzien und Alkohole optimiert, um maximale Auflösung und Proteinausbeute zu erzielen. Die löslichen Matrixproteine …
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Milk protein adulteration detection via mass spectra and infrared spectroscopy
… thesis aims to test and compare methods such as two-dimensional gel electrophoresis (2-DE), liquid chromatography tandem mass spectrometry, and infrared spectroscopy to detect foreign protein spiked in milk, especially hydrolyzed plant proteins. Here are the major results: (1) According to the …
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Impatto di rifaximina sul microbiota intestinale: selezione di bifidobatteri antibiotico resistenti
… Exclusion of any reversion phenomenon suggested two hypotheses: (i) stable and immobile genetic elements encode resistance; (ii) the drug moiety does not act as an inducer of the resistance phenotype, but enables selection of resistant mutants. Since point mutations in rpoB have been indicated as …