Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"two-component signal transduction"”.
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Specificity and evolution of bacterial two-component signal transduction systems
… capacity to sense and process a diverse range of signals. Duplication and divergence of a relatively small number of gene families has provided the raw material enabling cells to quickly increase their signaling capacity. After duplication, however, all pathway components are identical in sequence …
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Structural Characterization of Two-Component Signal Transduction Proteins and Calbindin D28k
… the structural analysis of proteins involved in signal transduction pathways. In the first study, the metal binding properties of the initiation of sporulation response regulator Spo0F was studied using a variety of biophysical and biochemical techniques. The experiments show that most of the …
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Characterization of the mechanisms of two-component signal transduction involved in motility and chemotaxis of Helicobacter pylori
… Sigma Faktor28-Antagonisten FlgM enthält. Das Signal, welches über die Histidinkinase des Zweikomponentensystems HP244/FlgR die Expression der Sigma Faktor54-abhängigen Klasse 2 Flagellengene reguliert, ist bisher noch nicht bekannt. Allerdings konnte mit HP137 ein Protein identifiziert werden, …
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A novel two-component signal transduction system in propionibacterium acnes and its association with a putative extracellular signalling peptide
… often involved in pathogenecity of bacteria, its signal transduction pathway has been suggested as potential target for new drug development. This project identified a putative unique quorum sensing system of P. acnes, consisting of a putative signalling peptide, and divergently transcribed …
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Examination of Molecular Recognition in Protein-Ligand Interactions
This dissertation is a compilation of two main projects that were investigated during my thesis research. The first project was a prospective study which identified and characterized drug-like inhibitors of a prototype of bacterial two-component signal transduction response regulator using …
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Role of the Nla6S and Nla28S histidine kinases in fruiting body development of <i>Myxococcus xanthus</i>
… structures known as fruiting bodies. Two component signal transduction systems (TCS) are widely used by bacteria to detect and respond to environmental cues by regulating large-scale changes in gene expression. They contain a histidine kinase sensor that detects environmental cues and …
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Physiological and genetic evidence for an OmpB signal transduction system in Erwinia chrysanthemi
… in their environment they must continuely sense signals such as, presence of host organisms, chemical concentrations, or variationsin other physiological parameters. Many bacteria sense their environment through the use of a two component regulatory systems. These systems usually employ the use …
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Regulation of amylovoran biosynthesis in Erwinia amylovora
… diseases for the apple and pear industry. Two pathogenicity factors strictly required for E. amylovora to cause disease are the exopolysaccharide amylovoran and the type III secretion system. In bacteria, the two-component signal transduction (TCST) system is the major paradigm to sense …
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Phenotypic variation in Photorhabdus temperata K122
… spp. have the ability to exist in either of two phenotypically different forms. The bacterial form isolated from the nematode is referred to as the primary phenotype variant, whilst the phenotypically different form is referred to as secondary phenotype variant. This secondary variant is …
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Extracytoplasmic stress response systems in S. Typhimurium
… response systems that target stresses affecting components of the cell envelope such as the periplasm and outer membrane proteins. The two best characterised ESRs are the RpoE stress response system and the CpxAR two-component signal transduction system. Two further ESRs, the BaeSR response and …
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Further characterisation of the envelope stress responses of Salmonella Typhimurium
… transcriptomic investigation into the ZraSR two-component signal transduction system and characterisation of its accessory protein, ZraP. We suggest ZraR mediated positive regulation of the virulence factors MntH and GroEL, GroSL, DnaK and ClpB (the heat shock chaperones). In addition, we …
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Understanding the Genetics and Evolution of Antimicrobial Resistance in Escherichia Coli
… coli. Finally, to gain understanding of the network of genes contributing to intrinsic and phenotypic resistance, I have studiedE. coli's two-component signal transduction systems (TCS). TCSs are involved in bacterial responses to many stresses, including antibiotics. I examined interactions …
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Analysis of alkali-inducible genes of Bacillus subtilis
… more unknown regulator(s). By their signature, two of them kipR and yvdT code for regulatory proteins, while pspA, member of the sigmaW regulon, encodes another potential regulator. In this doctoral work, the genes kipR, yvd and pspA were analyzed. The kipR and yvdT genes code for a …
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Butyrate Sensing by Campylobacter jejuni Impacts Bacterial-Host Interactions
… identified and characterized the C. jejuni BumSR two-component signal transduction system (TCS) that specifically directs a response to butyrate. Deletion of either C. jejuni bumS or bumR abolishes butyrate-modulated transcriptional changes in gene expression. Analysis of ΔbumS and ΔbumR mutants …
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Going Beyond Phosphorylation: Acetylphosphate-Mediated Ran Polymerase Acetylation and Caper Phosphorylation Co-Regulate Transcription of Escherichia Coli Stress-Responsive Gene cpxP
… extracellular conditions is a group of signaling pathways known as two-component signal transduction system (2CST). These systems relay information from the extracellular environment to the interior of the cell via a transfer of phosphoryl groups from the sensory protein know as sensor …
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Probing the assembly of the <em>Bacillus subtilis</em> flagellum and its role in signal transduction.
… temperature. As such, bacteria have a number of signal transduction mechanisms at their disposal that allow them to detect a range of different stimuli, integrate different signals and react to them appropriately. The work presented in this thesis aimed to understand more about the signalling …
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Characterization of major virulence regulators of Erwinia amylovora
… and type III secretion system (T3SS) are two major virulence factors in E. amylovora. However, how these virulence factors are regulated is not completely understood. In bacteria, gene expression is mainly regulated at the transcription initiation level and its core RNA polymerase (RNAP) …
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Regulation of clostridium acetobutylicum glutamine synthetase glnA gene
… the family of the response regulators involving two component signal transduction systems, suggesting that the protein encoded by this ORF may play a role in the mechanism of regulation of the C. acetobutylicum glnA gene in response to nitrogen.
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MUTACIN IV PRODUCTION IN STREPTOCOCCUS MUTANS UA159: CHARACTERIZATION, REGULATION, AND MECHANISM OF SELF-IMMUNITY
… that heterologous expression of SMU.152 in two sensitive strains converted the strains to become resistant to mutacin IV. We identified that C-terminal charged residues of SMU.152 is indispensible for conferring immunity to susceptible cells. In addition, our studies demonstrated that in …
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Overexpression, purification and ligand interaction studies of intact membrane sensory kinases from enterococci: towards agonist and antagonist screening
Two component signal transduction systems are of great importance in the regulation of expression of many virulence traits in bacteria and are a vital component of the way bacteria sense and respond to their environment. The sensor kinase component is generally membrane bound and is involved in …
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