Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"two photon imaging"”.
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NANOCOMPOSITES CONTAINING GOLD NANORODS FOR TWO-PHOTON IMAGING, PHOTODYNAMIC THERAPY AND DRUG DELIVERY
Nanocomposites, in which two or more different nanosized entities arranged in a controlled manner, have been widely investigated in recent years. This nanocomposite combines the properties and advantages of each component, exhibiting multifunctionality or novel properties that are not found in …
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On the fundamental imaging-depth limit in two-photon microscopy
One of the principle advantages of two-photon microscopy over one-photon techniques is that it can provide high-resolution images from very deep within living tissue. While imaging depths of 500 micron in brain tissue have become standard performance, larger depths have been inaccessible mainly due …
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Large Two-photon Absorption of Highly Conjugated Porphyrin Arrays and Their in vivo Applications
<p>Two-photon excited fluorescence microscopy (TPM) has become a standard biological imaging tool due to its simplicity and versatility. The fundamental contrast mechanism is derived from fluorescence of intrinsic or extrinsic markers via simultaneous two-photon absorption which provides inherent …
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Imaging Chloride Homeostasis in Neurons
… mutagenesis screening, protein engineering and two-photon-imaging this thesis work led to the discovery of new chloride-sensitive GFP mutants and to the establishment of ratiometric imaging procedures for the quantitative combined imaging of intraneuronal pH and chloride. These achievements have …
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Design and Development of Multiscale Microscopic Imaging Systems for Neuroscience Applications
Multiscale imaging systems enable the measurement of micro-scale activity from individual neurons and mesoscale activity from diverse brain regions for understanding functional properties and correlations of widely separated neurons. However, these techniques are often expensive, with complex …
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Phase-sensitive light : coherence theory and applications to optical imaging
… (SPDC) can produce pairs of entangled photons, i.e., a stream of biphotons. SPDC has been utilized in a number of optical imaging applications, such as optical coherence tomography, ghost imaging, holography and lithography, to obtain performance that cannot be realized with standard …
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Habituation and Dishabituation in the Olfactory Bulb: From Neural Responses to Behavior
… and behavioral olfactory dishabituation. Calcium imaging was used to determine the timecourse and magnitude of habituation in different parts of the OB during and after a prolonged odor presentation. Widefield imaging of the dendritic, or glomerular, response of OB output cells demonstrated that …
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Compact Femtosecond Lasers for Multiphoton Imaging
Multiphoton imaging currently is one of the main development directions in Laser Scanning Microscopy. A simplified way to assess suitability of a given laser for MPI applications is a figure of merit (FOM) approach based on peak power and average power of the particular excitation laser used. The …
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Acetylcholine dynamics in the retrosplenial cortex during spatial uncertainty
… for spatial navigation and the default mode network, integrating egocentric and allocentric inputs, contextual information, and multiple spatial hypotheses. This positions the RSC as a key region for investigating how the brain represents uncertainty. Computational theories propose that …
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Advanced technologies for spatio-temporal control of neural circuits using optogenetics
… recordings and advanced optical imaging is leading to promising approaches aiming at obtaining information on spiking activity, morphology and genetic identity of the many constituents of neural networks.;This PhD research project focussed on the development of an experimental …
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Longitudinal tracking of neural vascular recovery post microinfarct using multimodal neural platform
… used ultra-flexible nanoelectrode thread probes, two-photon imaging, and speckle imaging to track neural activities, microvascular structure, and regional cerebral blood flow longitudinally post-stroke. Our findings reveal several important insights about this transient local damage in an aged …
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Experience-dependent dendrite remodeling of GABAergic interneurons in the adult visual cortex
… adult plasticity. Using in vivo two photon imaging, we monitored intemeuron dendritic branch tip remodeling in response to changes in visual experience in the adult mouse visual cortex. We find that branch tip dynamics are induced by novel experiences in a stimulus-specific …
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Visualizing inhibitory and excitatory synapse dynamics In vivo
… To address this issue, we utilized triple-color two photon microscopy to examine inhibitory and excitatory synapses across time with daily imaging. We found that the majority of dynamic spines at these intervals lacked a mature excitatory synapse as indicated by the absence of PSD-95. Inhibitory …
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The hemo-neural hypothesis : effects of vasodilation on astrocytes in mammalian neocortex
… their participation in traditional signaling networks with other neurons. Mechanically sensitive astrocytic endfeet ensheathe cerebral blood vessels, which change size in order to regulate blood flow. We found that changes in local biophysical state caused by mechanical perturbations exerted …
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Inhibitory synapses are repeatedly assembled and removed at persistent sites in vivo
… brain in vivo, we used dual- and triple- color two-photon imaging to track the dynamics of all inhibitory and excitatory synapses onto a given neuron in the cerebral cortex at different timescales. By studying synaptic changes over 4-day or 24-hour intervals we were able to determine that …
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Role of Intracellular Calcium Stores and Calcium-Permeable AMPARs in Functional and Structural Plasticity in Mouse Hippocampal Slices
… depression (LTD). LTP can be differentiated into two forms based on their dependence (LTP2) or independence (LTP1) on protein synthesis (PS) and are hypothesized to be analogous to LTM and STM respectively. In both forms of LTP, calcium (Ca2+) signaling plays a vital role. However, Ca2+ has a …
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Multiphoton Endoscopy
Multiphoton microscopy has the potential to be a versatile tool for clinical disease diagnosis and treatment, providing numerous benefits over current practices involving the histopathological analysis of biopsies. Specifically, multiphoton excitation has the unique ability to generate fluorescence …
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Spatiotemporal Dynamics of Tissue Oxygenation in Awake, Behaving Mice
… activity is prolonged, indicating at least two primary control mechanisms for an early and late phase. However, it is unclear if the early and late components of the hemodynamic response are differentially regulated across space and time. Here, I test the hypothesis that behavioral state …
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