Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 177 for “"tumour suppressor"”.
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Investigation of candidate imprinted tumour suppressor genes
Imprinting is an epigenetic phenomenon that silences one allele of a gene, so that expression in one or more cell types is exclusively monoallelic, and dependent on parental origin. Approaches used to identify novel imprinted genes rely on characteristic features such as the clustering of imprinted …
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Structure of the LKB1-STRAD-MO25 tumour suppressor complex
A landmark study published over ten years ago established a link between mutations of the LKB1 gene and the Peutz-Jeghers cancer syndrome. Since then, much effort has been devoted to understanding the functional roles of the protein encoded by the LKB1 gene. LKB1 is a Ser/Thr kinase that is …
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Studies of the PTEN tumour suppressor in endometrial cancer.
… up-regulation of PTEN represent potential anti-tumour therapies and therefore merit investigation. To further investigate the role of PTEN in endometrial cancer the following approaches were taken. The effect of TGF-beta1 on two endometrial carcinoma cell lines, HEC-1B and Ishikawa were …
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Developing nanobodies to stabilise the tumour suppressor protein p16INK4a
The tumour suppressor protein p16INK4a (p16) is a cyclin-dependent kinase (CDK) inhibitor that plays a key role in the regulation of the cell cycle by controlling the progression of cells through the G1 to S phase transition. Dysregulation of the protein through deletion, silencing or mutation of …
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Stability, folding and evolution of the tumour suppressor protein p16.
The tumour suppressor protein, p16, is a member of the INK4 family of inhibitors of the cyclin D-dependent kinases 4 and 6 in the pRb pathway of eukaryotic cell cycle. The 156-amino acid protein consists of four ankyrin (ANK) repeats. The ANK repeat is a ubiquitous 33-residue sequence motif that …
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Development of Bioluminescent Biosensors Monitoring Activity of the Merlin Tumour Suppressor
All solid tumours share the ability to circumvent or overcome contact inhibition of proliferation (CIP), the process by which cell-cell contacts engage signalling to stop proliferation. Merlin, an ERM-like protein, plays a crucial role in CIP and is inactivated in a wide variety of cancers, …
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A tumour suppressor role for the T-box transcription factor TBX3 in fibroblasts
… also able to significantly increase the in vivo tumour forming ability of HT1080 cells in a mouse model. A TBX3 overexpression cell culture model was also generated in the HT1080 cells and, despite their aggressive nature, increased TBX3 expression resulted in a reduced oncogenic phenotype, …
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A Role for Insulin Signaling in Regulating the PTEN Tumour Suppressor in Caenorhabditis Elegans
… pathway, and is the second most commonly mutated tumour suppressor (after p53) found in human cancers. In many cancers the PTEN gene is not deleted, but instead the protein is lost. Therefore the regulation of PTEN protein in humans is of great importance. Here we hypothesized that the activated …
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Suppression and activation of lipid signalling in cancer : tumour suppressor PTEN and oncogenic sphingosine kinase
The PTEN tumour suppressor is the second most frequently mutated tumour suppressor gene in cancer. It is a lipid and protein phosphatase that negatively regulates the well-known pro-proliferative and anti-apoptotic phosphatidylinositol 3-kinase (PI3K)/AKT signalling pathway to modulate cell …
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Morphogenesis of follicular epithelium in Drosophila melanogaster: function of von Hippel-Lindau tumour suppressor gene
… h-VHL functions that are relevant to tumour development, including maintenance and organization of the primary cilium, maintenance of the differentiated phenotype in renal cells and regulation of epithelial-mesenchymal transition. However, the function of h-VHL gene during development …
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G2/M checkpoint associated repression of polo-like kinase-1 mediated by the tumour suppressor, p53
Polo-like Kinase-1 (PLK1) is an important mediator of the G2/M phase of the cell cycle that is down-regulated in a DNA damage dependent manner. In cancer cells PLK1 is overexpressed and allows for continued proliferation of the cell by overriding this checkpoint. Here I show that PLK1 is …
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Modulation of microRNA Functions by the 3’ Untranslated Regions of CD44 and Tumour Suppressor Candidate 2
… over-expression of two 3’UTRs, CD44 and Tumour Suppressor candidate 2 (TUSC2), on miRNA functions in breast cancer carcinogenesis were investigated. A series of cell function assays in human and mouse breast cancer cell lines, MT-1, MDA-MB-231 and 4T1 showed phenotypic changes caused by …
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Novel RNA-based biomarkers for ovarian cancer – uncovering how LINC01132 is regulated by the tumour suppressor protein, p53
… ovarian cancer cases have a mutation in p53, a tumour suppressor transcription factor which counteracts cell stress and oncogenic signals. Most mutations occur within p53’s DNA-binding domain, a vital region which facilitates its anchorage to target gene promoters. Previous data found numerous …
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Defining the role(s) of non-classical tumour suppressor Wwox in cellular function using Drosophila melanogaster genetic modelling
… cellular pathways, however the role of WWOX in tumourigenesis is not yet fully defined. There is therefore a need to determine the normal function(s) of WWOX and how perturbation of these roles is likely to contribute to cancer. A model was previously established to examine the cellular function …
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An investigation into the regulation and expression of the tumour suppressor gene clusterin in oral, cervical and nasopharyngeal cancer
… RNASET2 and CLU were identified to be candidate tumour suppressor genes in cervical neoplasia and subsequent validation led to an evaluation of the regulation and expression of CLU at three sites of squamous cancer: the oral cavity, cervix and nasopharynx. Down-regulation of CLU was demonstrated …
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The candidate tumour suppressor, XIAP associated factor 1 (XAF1), directly inhibits XIAP activity and induces G1 phase cell cycle arrest
… in numerous types of cancers, this may suggest a tumour suppressor role for XAF1 in cancer. To test this theory, a 60 cell line panel from the NCl was analyzed for xaf1 RNA expression by Taqman and heterozygosity status of markers proximal to xaf1. Taqman analysis indicated that the majority of …
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Functional analysis of ANKRD11 and FBXO31: two candidate tumour suppressor genes from the 16q24.3 breast cancer loss of heterozygosity region.
… identified both ANKRD11 and FBXO31 as candidate tumour suppressor genes in the chromosome band 16q24.3, which is the smallest region of overlap for breast cancer LOH. This thesis focuses on the functional analysis of these two novel genes and implicates a role for them as breast cancer tumour …
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Gene mutations and expression in breast cancer
… disorder with mutations in oncogenes and tumour suppressor genes playing an important role in cellular transformation and ultimately in tumour formation. In this study, 40 breast cancer patients from the Western Cape province in South Africa and 4 breast cancer cell lines were screened for …
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