Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"tryptophan fluorescence quenching"”.
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Mapping RNA Binding Surfaces on Hfq Using Tryptophan Fluorescence Quenching
… more complex RNA sequences interact with Hfq, a tryptophan fluorescence quenching (TFQ) assay has been developed. Here it is presented that TFQ can correctly identify the binding face for two control sequences, A15 and U6, using the E. coli, S. aureus and L. monocytogenes Hfq homologues. Using …
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Investigation of the dynamics and conformational changes of the U1A protein
… RNA complex using laser T-jump and stopped-flow fluorescence methods. Analysis of the kinetic data suggested three phases of dissociation with two intermediate states. Chapter 4 is a follow up study of chapters 2 and 3 to further investigate helix C dynamics. The experimental design makes use of …
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Role of the Lipid Environment and Cholesterol Content in the Function of CFTR Modulators
… while circular dichroism spectroscopy and tryptophan fluorescence quenching experiments suggest that cholesterol promotes the helical fold of the constructs in the membrane. Furthermore, the potentiator Ivacaftor’s interaction with the membrane is highly enhanced in the presence of even …
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Probing Structural Differences of Recombinant Prion Isoforms Using Fluorescence Spectroscopy
… of prion diseases. In this study, six single-tryptophan mutants of recombinant PrP were expressed, purified, and refolded into PrPC or two misfolded isoforms of prion, PrPb and PrPF. Solvent accessibilities of the six tryptophan residues were probed among the three isoforms using various …
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Probing Structural Differences of Recombinant Prion Isoforms Using Fluorescence Spectroscopy
… of prion diseases. In this study, six single-tryptophan mutants of recombinant PrP were expressed, purified, and refolded into PrPC or two misfolded isoforms of prion, PrPb and PrPF. Solvent accessibilities of the six tryptophan residues were probed among the three isoforms using various …