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Showing 1 to 12 of 12 for “"tryptic peptide"”.
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Immunochemical studies with tryptic peptides of tobacco mosaic virus protein
… determinants. Two of these, situated in tryptic peptide I (residues 1-41), were found to be a cryptotope (i.e. an antigenic determinant absent from the outer surface of the assembled viral capsid) and a viral antigenic determinant. Tryptic peptides 4 (residues 62-68) and 8(residues …
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Transcriptional and Translational Products of Avian Erythroblastosis and Avian Myeloblastosis Viruses
… gag gene at its amino terminus. Two-dimensional tryptic peptide mapping indicated that p75 contained novel peptides in addition to those derived from p19. p75 was found to be a phosphoprotein containing a phosphoserine residue. Two-dimensional tryptic phosphopeptide maps of p19 and p75 were …
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Characterization of Tumorigenic Proteins
… and transformed cells were analyzed by tryptic peptide mapping on ion exchange columns. The tryptic peptides were mapped relative to the SV40 DNA and to their known functions. The aminotermini of the 17K, 84K and 94K T antigens are identical. The 84K protein probably arises from the 94K …
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Development of Methods for the Study of Phosphoproteins
… protein identification technology) and phosphopeptide isolation. This column, a poly(AMPS-co-PEGDA) monolith containing as high as 40% AMPS, has several favorable features, such as high binding capacity, extraordinarily high resolution, and high peak capacity, making it ideal for resolving …
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A Biochemical Study of Gossypol and Lactate Dehydrogenase X Binary Interactions
… chromatography (HPLC) separation of the LD-X tryptic peptide fragments to observed alterations in separation patterns as indicators of intramolecular disturbance in the enzyme molecule. Definite alterations in peptide fragment peaks were observed in the presence of gossypol and suggest …
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Mass Spectrometric Characterization and Fluorophore-Assisted Light Inactivation of Human Excitatory Amino Acid Transporter
… of the protein was achieved. An 88-amino acid tryptic peptide covering the proposed substrate binding site was revealed after N-deglycosylation. This study provided an efficient and simple method to purify, digest and characterize integral membrane proteins by MS. In addition, the EAAT2 peptide …
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Mass Spectrometric Characterization and Fluorophore-Assisted Light Inactivation of Human Excitatory Amino Acid Transporter
… of the protein was achieved. An 88-amino acid tryptic peptide covering the proposed substrate binding site was revealed after N-deglycosylation. This study provided an efficient and simple method to purify, digest and characterize integral membrane proteins by MS. In addition, the EAAT2 peptide …
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Regulation of natriuretic peptide system activation:effects of spontaneous natriuretic peptide mutations and identification of novel natriuretic peptide receptor phosphorylation sites.
… mediate the physiologic effects of natriuretic peptides, including reduction of blood pressure and blood volume, stimulation of long bone growth, and regulation of cardiac size. Atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) signal through GC-A while C-type natriuretic …
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Development and application of OmpT-based middle down proteomics
… analysis (LC-MS/MS) of post-digestion peptides. Top-down proteomics omits the proteolysis step and directly focuses on the characterization of intact proteins and their post-translational modifications. While both approaches continue to mature, they each have limitations. In the …
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Development and Application of Large-Scale Protein Folding Stability Analysis in Drug Target Identification and Disease Biomarker Discovery
… enrichment strategy to isolate the semi-tryptic peptides generated in mass spectrometry-based applications of limited proteolysis methods. The method is termed Semi-Tryptic Peptide Enrichment Strategy for Proteolysis Procedures (STEPP). The STEPP-PP workflow was evaluated in two …
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Multidimensional liquid chromatographic separations for proteomics
… both at an intact protein and digested peptide level. For complex lysates containing many thousands of proteins, orthogonal (mutually independent), multidimensional separation methods must be employed to provide sufficient resolution to characterize the appropriate number of different …
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The amino acid sequence of the major alpha globin of the rat (Wistar, Rattus norvegicus)
… chain was substantially determined. The soluble tryptic peptides prepared from the native ∝ globin and from the aminoethylated ∝ globin were separated by peptide mapping. Sequencing of the tryptic peptides was carried out by the dansyl-Edman method and by the overlapping of smaller peptide …