Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"tryptase"”.
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Sphingosine-1-phosphate in mast cell-mediated allergic responses
… cell factor (SCF) alone express intragranular tryptase (MCT), the phenotype predominant in the lung. S1P accelerated the development of cord blood-derived mast cells (CB-MCs) and strikingly increased the numbers of mast cells expressing chymase. These mast cells have functional FcepsilonRI, and …
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Bestimmung der Struktur der 15. Domäne des Multidomäneninhibitors LEKTI mittels NMR-Spektroskopie und Design einer inhibitorisch wirksamen Mutante
… legen eine Fehlregulierung der Proteinase Tryptase nahe, da diese eine Schlüsselrolle bei Überempfindlichkeitsreaktionen und Asthma einnimmt. Bei einem Sequenzvergleich des bislang einzig bekannten natürlich vorkommenden Tryptase-Inhibitors LDTI (leech derived tryptase inhibitor) mit den …
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Mast Cell Influence in the Progression of Post-burn Hypertrophic Scar Pathophysiology
… postburn scars concomitant with elevated serum tryptase concentrations over time in pediatric burn patients. Using in vitro approaches, we demonstrate tryptase’s ability to activate proliferation in primary post-burn HTS fibroblasts compared to that of non-burned skin fibroblasts. Tryptase, an …
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The role of n-linked glycosylation and sialylation in regulating human proteinase-activated receptor 1 and 4 expression and signalling
… found that activation of hPAR-2 by mast cell tryptase can be regulated by receptor N-terminal glycosylation. However, the role of N-linked glycosylation in regulating the expression and function of other PARs is not known.
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The study of the role of mast cells in malignant pleural effusion
… encounter. To achieve this, mast cells secreted tryptase AB1 and interleukin-1β, that, in turn, potently triggered vascular hypermeability and tumor cell nuclear factor-κΒ activation, respectively. cKIT inhibitor imatinib mesylate was effective against effusion development, indicating that mast …
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Peanut allergy: a prospective study of thresholds, co-factors, mediators and severity
… numerical scale derived from symptoms and serum tryptase was measured at each challenge. A total of 187 challenges were performed. Findings were that the median amount of peanut protein which induces a reaction in 10% of the population (ED10) was 12.3mg (95% CI 7.3,20.4) equivalently this …
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Effector Cells and Mechanisms in Chronic Spontaneous Urticaria
… of pro-inflammatory mediators (histamine, tryptase) and IL-6 in the skin, local histamine release and, possibly, neutrophil infiltration in the dermis. To test this hypothesis, we carried out cutaneous microdialysis of autologous serum skin test (ASST) response, a prospective study of …
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Neue Enzyminhibitoren und Rezeptoragonisten durch Variation funktionaler Schleifen von Mikroproteinen
… Design potente Inhibitoren der humanen Mastzell-Tryptase entwickelt werden, die alle vier Monomere der tetrameren Serin-Protease blockieren. Als technische Basis für die Herstellung der Mikroprotein Varianten in korrekt gefalteter Form wurde ein neuartiges Expressions- und Reinigungssystem …
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Role of mast cells in women's health and disorders of the endometrium
… regulation by steroids. Mast cell proteases tryptase and chymase were detected by RTPCR and immunohistochemistry in “full thickness” (uterine lumen to endometrial-myometrial junction) biopsies from women undergoing hysterectomy. In agreement with previous findings MCs were most abundant in …
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Bioactivity evaluation and structural modification of Bowman-Birk type protease inhibitory peptides from amphibian skin secretions
… and K16-PPF-BBI displayed excellent trypsin and tryptase inhibitory activity and antimicrobial activity. When the lysine residue at P1 site was replaced by phenylalanine, the forming peptide F8-BBI-PPF exhibited strong chymotrypsin inhibitory activity but totally lost the trypsin inhibitory …
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ROLES OF MAST CELL G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2) IN ALLERGY AND INFLAMMATION
… 48/80 (C48/80) but significantly reduced tryptase release in response to the Cathelicidin LL37. Furthermore, absence of GRK2 resulted in increased cutaneous vascular permeability in response to C48/80 in vivo. By contrast, LL37-injection induced rosacea-like erythema, generation of matrix …
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Expression, Purification, and Characterization of the Mast Cell Proteases Chymase and Cathepsin G.
… eicosanoids, and the serine proteases, tryptase, Chymase, and cathepsin G (CatG). These proteases are stored and released from mast-cell granules upon activation by antigen binding to IgE immunoglobulins on the cell surface or by direct injury. In this study, chymase and CatG were …
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EGFR deficiency results in defective hair follicle cycling, resistance to chemotherapy-induced alopecia, and cutaneous inflammation
… including the mast cell proteases Chymase and Tryptase and class I and II MHC molecules, consistent with a role for both innate and adaptive immunity in cutaneous inflammation following asynchronous catagen. The mitotic regulators Rcc2 and Sfi1 were increased correlating with our observation of …
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The role of intracellular and extracellular cysteines in regulating human proteinase-activated receptor-2 (hPAR2) function
… have found that activation of hPAR2 by mast cell tryptase, (the major PAR2 activator outside the gastrointestinal tract) can be regulated by receptor N-terminal glycosylation. In order to elucidate other post-translational modifications of hPAR2 that can regulate function, we have explored the …
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Characterisation of protease activated receptors
… *in vivo* namely, pancreatic trypsin, mast-cell tryptase and acrosin. Thrombin is a serine protease that plays a central role in blood coagulation. Its specificity is determined by interactions with both the active site and a region distant from the active site called the anion-binding exosite. A …
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Mass spectrometry-based protein profiling strategies for biomarker discovery in liver and inflammatory bowel diseases
… process. Among them heat shock protein 70, tryptase alpha-1 precursor and proteins whose upregulation can be explained by the increased activity of IECs in Crohn’s disease were identified. Follow-up studies for the validation of the results and the in-depth investigation of the inflammation …