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Showing 1 to 20 of 44 for “"trypan blue"”.

  1. The Effect of Trypan Blue on the Growth Rate of the Chick Embryo During Development

    Watkins, Edgar H.Drake University1972

    … embryo is changed by the teratogenic agent, trypan blue. Procedure. The eggs were divided into three groups, unopened controls, saline-injected (0.1 ml, 0.85 NaCl) standards and trypan-blue-injected (0.1 ml, 0.1% solution) experimentals. Injections were made into the yolk sac through the …

    drake Repository record

  2. Ischemic Preconditioning Protects Adult Rat Cardiomyocytes Against Necrosis but not Apoptosis, via Activation of PKG

    Caligtan, Marc J.2014

    … in the normal medium. Necrosis was determined by trypan blue exclusion and apoptosis was assessed by TUNEL assay. IPC reduced necrosis as shown by significant decrease in trypan blue positive cells as compared to virgin non-preconditioned myocytes subjected to SI and RO alone (p<.01). Similarly, …

    vcu Repository record

  3. The Role of PKC-δ in Hypoxia-induced Cerebrovascular Endothelial cell death.

    윤, 이슬2011

    … of released LDH (lactate dehydrogenase) and trypan blue positive cells during hypoxia after treatment with rottlerin, PKC-δ inhibitor. Hypoxia-induced LDH release was significantly increased by the treatment with rottlerin. We examined the specific blockade of PKC-δ activation by rottlerin …

    ajou Repository record

  4. Novel approaches to evaluate osteoarthritis in the rabbit lateral meniscectomy model

    Pease, Anthony P.Virginia Tech1997

    … 26, 40, and 57 with surgery occurring on day 12. Trypan blue was used in the lavage fluid to estimate the volume of harvested synovial fluid. There was a significant increase in the volume harvested on day 26 (p < 0.001). Superoxide dismutase concentration in synovial fluid increased after …

    vt Repository record

  5. Investigation of the genotoxic potential of the marine biotoxins okadaic acid and azaspiracids

    Dörr, Barbara ValentinaUniversity College Cork2014

    … two further endpoints were included. The Trypan Blue Exclusion assay was used to provide information on possible cytotoxicity and assess the right concentration range. Flow cytometer analysis was included to detect the possible involvement of apoptotic processes. In house background data …

    cork Repository record

  6. Investigation of the Effects of Genistein and Fenretinide on Ovarian Cancer Cells

    Azadi, BehnamUniversité d'Ottawa / University of Ottawa2012

    … were tested on A2780s and A2780cp cells using trypan blue viable cell count, MTS assay. Results and conclusions: Unlike low doses of fenretinide, genistein had anti-proliferative effects on both cell lines. There were no additive or synergistic effects of the two compounds. Higher dose …

    ottawa-retro Repository record

  7. An In Vivo Perfusion Apparatus for Studies on Gingival Disease : Assessment by a Perfusion of Nonspecific Proteolytic Enzymes

    Dornan, Don

    … first molar) on one side with tap water and trypan blue. Localization of the solution delivered and minimal histological changes due to the presence of the apparatus was demonstrated on these animals. A 1% solution of trypsin was perfused on the experimental area of one animal for one day at …

    loma-linda Repository record

  8. Identification and functional study of a novel bioactive peptide QUB-1761 from the venom of the Brazilian yellow scorpion, Tityus serrulatus

    Wang, XinyueQueen's University Belfast2021

    … In MTT anticancer cell proliferation and trypan blue exclusion assays, QUB-1761 beyond the concentration of 40 µM can completely kill the human lung cancer cells (NCI-H838). In terms of the haemolytic assays, QUB-1761 showed about 30% haemolysis activity at 64 µM and more than 50% at 128 …

    qu-belfast Repository record

  9. Role of Extracellular Signal­-Regulated Kinase and Peroxisome Proliferator-­Activated Receptor gamma on Transforming Growth Factor-­β1-­Induced Human Endometrial Stromal Cell Decidualization

    장, 혜진2011

    … were added according to experimental purposes. Trypan-blue and a hemocytometer were utilized to count the viable cell number. Enzyme-linked immunosorbent assay (ELISA) and Western blotting was employed to detect proteins. RESULTS: TGF-β1 inhibited proliferation of cultured human endometrial …

    ajou Repository record

  10. Neuroprotective Effects of Propofol and Ketamine(alone or combined) Against Oxygen-Glucose Deprivation-Reperfusion Injury

    최, 정웅2011

    … (1, 2.5, 5, 10, 50μM) 을 각각, 혹은 동시에 투여하였다. Trypan-blue 염색을 한 후 살아남은 세포의 수를 세어 세포 사망률을 계산하고 oneway-ANOVA test 를 실시하였으며, Bonferroni's test 를 통하여 사후 검증을 실시하였다. 연구 결과 : 허혈-재관류 손상 후 약 70% 의 세포사망률을 보였으며, 5−50μM 의 ketamine 을 투여한 실험군 에서는 세포사망률이 유의하게 감소하였고, 10−100μM 의 propofol 을 투여한 실험군 에서도 유의한 …

    ajou Repository record

  11. Effects of survivin and survivin inhibition in canine models of lymphoma and osteosarcoma

    Shoeneman, Jenette K., author, Thamm, Douglas, advisor, Gustafson, Daniel, committee member, Duval, Dawn, committee member, Biller, Barbara, committee member, Ehrhart, EJ, committee memberColorado State University. Libraries2014

    … was assessed via manual cell counting with trypan blue. Cellular apoptosis was confirmed via caspase-3/7 and TUNEL assays. Cell cycle analysis was performed with propidium iodide staining followed by flow cytometry. Chemosensitivity to doxorubicin (DOX) was also assessed with caspase-3/7 …

    colostate Repository record

  12. The Effect of Reticulo-Endothelial Blockade on the Capillary Resistance Responses to Cortisone and Sth

    O'Brien, Richard L.Creighton University1958

    … investigators showed that R.E. blockade with trypan blue abolished the eosinopenic response to ACTE (6). | This result has since been disputed (8,9,18) but one of these disputing papers reported abolition of stress induced eosinopenia by R.E. block (18). | In view of the contrasting …

    creighton Repository record

  13. Oxygen diffusion limitations in pancreatic islet culture and immunoisolation

    Avgoustiniatos, Efstathios SMassachusetts Institute of Technology2002

    … loss of viability data, obtained using Trypan blue staining,

    mit Repository record

  14. Exploring the in vitro effects of chronic methamphetamine exposure on selected cancer cell lines

    Sasman, Sasha-leeUniversity of the Westen Cape2025

    … and proliferation were assessed using the trypan blue exclusion assay. Mitochondrial activity was evaluated using the XTT assay. Intracellular reactive oxygen species (ROS) levels were quantified using the H₂DCFDA assay. All measurements were analysed relative to controls to identify dose- …

    western-cape Repository record

  15. Bioactivation of diacetyldapsone in cultured lung cells

    Nimbalkar, Dipali2018

    … cultured CRL 7272 lung cells was assessed by the trypan blue dye exclusion technique. There was no significant reduction in cell number as compared to the control for incubations with three different concentrations of CD-DADDS complex. The amount of arylamine produced by hydrolysis was initially …

    u-pacific Repository record

  16. The Role of Reactive Oxygen Species in Crotalus atrox Venom-induced Cell Death

    Brown, Lindsay2021

    … atrox</em> (CA) venom for 4 and 10 hours. Alamar Blue assays were used to measure cell viability and results suggest a 15±8.6% (p<0.05) and 59±10.7% (p<0.05) reduction in cell viability at 4 and 10 hours, respectively. Cells stimulated with 500μg/ml venom for 10 hours stained 98±2.2% (p<0.05) …

    kennesaw Repository record

  17. Protective effects of Human Bone Marrow-Derived Mesenchymal Stem Cells on Apoptosis Induced Human Neuroblastoma SH-SY5Y Cells

    신, 진영2011

    … SH-SY5Ys. Cell viability was assessed by trypan blue dye exclusion method and MTS assay for 5days. Morphological, biochemical and Bcl-2 family western blot analysis were also performed. Number of viable cells was decreased progressively in STS-treated SH-SY5Ys in time, …

    ajou Repository record

  18. The Effects of PPAR-γ Activators on the Growth and Apoptosis of Melanocytes

    이, 중선2011

    … of 15d-PGJ₂, cytotoxicity was observed by trypan blue assay. Minimal, not statistically significant, increase of melanin content was observed in treatment with troglitazone and 15d-PGJ₂. Statistically significant differences were seen on ciglitazone-treated cells. Ciglitazone also induced …

    ajou Repository record

  19. The effects of catalase and kisspeptin overexpression on amyloid peptide toxicity

    Chilumuri, A.University of Westminster2014

    … 5-diphenyltetrazolium bromide) or trypan blue assay. Catalase overexpressing SH-SY5Y neurons showed a reduced susceptibility to Aβ and H2O2 toxicity compared to vector control cells. The catalase overexpression neuroprotection could by reduced by the catalase activity inhibitor …

    westminster Repository record

  20. The effects of superoxide dismutase on osteoarticular cells and structural analysis of ochronosis, in a disease model of alkaptonuria

    Kammath, Vishnu, Taylor, Adam, Bukhari, MarwanLancaster University2017

    … up to 14 days. Cell viability was assessed using trypan blue assay. Pigmentation was quantified using Schmorl’s staining technique. A dimethylmethylene blue assay was performed to assess damage to proteoglycans. Structural analysis was then performed on an in vitro model of ochronosis via Raman …

    lancaster Repository record

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