Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"trpc"”.
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Pharmacological regulation and function of store-operated calcium channels
… all types of mammalian cell. STIM1, ORAI and TRPC have been regarded as the molecular basis of SOCE. Once the endoplasmic reticulum (ER) Ca²⁺ store is depleted, STIM1 proteins move to the plasma membrane and activate ORAI and TRPC channels to allow Ca²⁺ influx. In this thesis, the …
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Role of transient receptor potential channels in mammalian oviduct and uterine epithelia
… The aim of this study was to discover which TRPC isoforms are expressed in epithelial cells lining the female reproductive tract in the bovine and human. Gene expression of TRPC channels changes was measured throughout the oestrous cycle in bovine oviduct and uterine epithelial cells using …
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Receptor-mediated calcium entry in retinal amacrine cells
… an immunocytochemical localization of two TRPC channel subunits, TRPC1 and TRPC4. TRPC1 expression was observed in amacrine cells and their process in the chicken retina. TRPC4 expression was much more widespread with some degree of labeling found in all layers of the retina, and was shown …
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Neurotoxic effects of malathion and lead acetate on the blood-brain barrier: Disruptive effects caused by different mechanisms examined with an in vitro blood-brain barrier system
… transient receptor potential canonical channels (TRPC) that in turn increase intracellular calcium levels affecting tight junction structure. The hypothesis was tested by assessing lead-malathion interactions in an in vitro BBB model. This model was constructed with rat astrocytes and rat brain …
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Role of the Transient Receptor Potential Channels in Modulating Prefrontal Cortical Excitability and the Behavioral Responses to Cocaine
… to be a canonical transient receptor potential (TRPC) channel. We used in situ hybridization, immunoblots, and real-time PCR to examine the expression of the TRPC channels and found dense expression of TRPC5 in the pyramidal cell layers of the PFC. Using adeno-associated viral mediated knock-down …
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New Techniques for Time-Reversal-Based Ultra-wideband Microwave Pulse Compression in Reverberant Cavities
… circulator (6.5 GHz to 17 GHz) to perform TRPC. Prior to this work, TRPC has only been demonstrated in electromagnetics using two or more feeds and a reverberant cavity acting as the time-reversal mirror. This new 1-port technique is demonstrated in both simulation and measurement. The …
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Expression et signalisation des intégrines des cellules glomérulées de la glande surrénale de rat : implication de composantes de la matrice extracellulaire, d'hormones et de canaux ioniques
… avons étudié la présence potentielle de canaux TRPC (transient receptor potential classical) dans les cellules glomérulées. Ces canaux sont des canaux cationiques non sélectifs permettant un influx de sodium, calcium et de césium. Des expériences d’immunohistochimie ont montré la présence de …
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The Role of Ca²⁺/CaM-Stimulated Phosphodiesterase 1C in Vascular Smooth Muscle cells of the Synthetic/Activated Phenotype
… including PDGF-alpha receptor, l-caldesmon, and TRPC1, while the expression of other markers, including PKG, vinculin, and beta-1 integrin were unaffected. Treatment with PDE1C siRNA also reduced cell migration, proliferation, and the ability of HASMCs to bind extracellular matrix proteins. These …
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Studies of calcium signalling mechanisms in human platelets
… as the transient receptor potential canonical 6 (TRPC6) channel. -- The main aim of this study was to examine the role of STIM1 and TRPC proteins in Ca2+ entry in human platelets. The TRPC1 protein was originally proposed to be a major component of the SOC channel based on the observation that a …
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Role of Trp Channels In Mediating The Calcium Signaling Response of Brain Endothelial Cells to Mechanical Stretch
… receptor potential (TRP) channel subfamilies, TRPC and TRPP, demonstrated a reduction of the stretch-induced Ca<sup>2+</sup> influx. RNA silencing directed at individual TRP channel subtypes revealed that TRPC1 and TRPP2 largely mediate the stretch-induced Ca<sup>2+</sup> response. In addition, …
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Development of Techniques for Analysis of the Human Retinal Ganglion Cell Transcriptome: Application to the Role of Calcium in RGC Death in Glaucoma
… induced RGC death. Two TRP channels, TRPM-2 and TRPC-3, which mediate Ca2+ influx, were found that predominantly expressed in the RGCL. Involvement in RGC death in the OGD model using the TRP inhibitor ACA could not be confirmed. Conclusions: The technique developed has enabled determination of …
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STIM Protein Coupling Domains and The Activation of Orai Channels
… 2-APB, DPB162-AE does not affect L-type or TRPC channels or Ca2+ pumps at maximal STIM1-Orai1 blocking levels. DPB162-AE blocks STIM1-induced Orai1 or Orai2, but does not block Orai3 or STIM2-mediated effects. We narrowed the DPB162-AE site of action to the STIM-Orai activating region (SOAR) …
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Vascular Mechanisms of Rhabdomyolysis-induced Acute Kidney Injury
… transient receptor potential canonical (TRPC) channels. TRPC1, 3, 4, 5, and 6 are expressed in renal vessels, with TRPC3 predominant. TRPC3, TRPC6, and TRPC7 are highly homologous. Although the ET system is known to be associated with rhabdomyolysis-induced AKI, the function of renal …
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Development of a Transformation System for Caldariomyces Fumago and Expression of Heterologous Proteins
… by promotion and termination sequences from the trpC gene of Aspergillus nidulans. Colonies which grew from the initial selection were shown to be transiently resistant as they did not grow upon reselection. Stable transformants were obtained when homologous C. fumago sequences were placed on the …
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Gating Mechanisms of The Canonical Trp Channel Isoform Trpc4
… by Transient Receptor Potential Canonical (TRPC) proteins play important roles in regulatory and pathophysiological processes. These channels are known to be activated downstream from phospholipase C (PLC) signaling. However, the mechanism by which the PLC pathway activates TRPC4/C5 remains …
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Structure-based development of novel chemical scaffolds as inhibitors of the store-operated calcium entry pathway
… against the SOCE (Orai-mediated) and non-SOCE (TRPCs-mediated) pathway. Recently, few structures of the Drosophila Orai (DmOrai) have been published and I sought out to identify novel SOCE-inhibitory chemical scaffolds utilising a more targeted approach utilising the available DmOrai structure. …