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Showing 1 to 20 of 38 for “"tropomyosin"”.
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Structure of Cocrystals of Tropomyosin and Troponin
… when calcium binds to troponin, causing it and tropomyosin to undergo conformational changes. Tropomyosin is an $\alpha$-helical protein with two chains (284 residues each) arranged as a coiled-coil. These rod-like molecules form cables wound in the grooves of the actin helix. Bound to each …
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Diffuse X-ray scattering from tropomyosin crystals
Motions in proteins are often an important part of their function. Structural information about the protein is hence incomplete without an understanding of the protein dynamics. X-ray crystallography is the technique used in the majority of protein structure determinations; from a crystallographic …
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Possible Roles for Tropomyosin During Anaphase Chromosome Movement
… to produce the force that moves chromosomes. If tropomyosin functions in the spindle, as it does in other actin microfilament systems, then it may regulate actin filaments along kinetochore fibres. The purpose of my thesis was to investigate whether tropomyosin has possible roles during anaphase …
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To Phosphorylate or Not to Phosphorylate: The Role of Tropomyosin Phosphorylation in Cardiac Function and Disease
Tropomyosin (Tm) is an α -helical coiled-coil protein crucial in the calcium dependent regulation of the thin filament of the sarcomere. α-Tm is phosphorylated solely at serine 283 and phosphorylation levels are tightly regulated. Approximately 70% of total cardiac Tm phosphorylated during fetal …
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Changes in the Expression of Thin Filament-Associated Proteins in Colonic Smooth Muscle from Mice During Inflammation
… associated proteins such as calponin, caldesmon, tropomyosin and smoothelin do not directly participate in contraction, they regulate acto-myosin interaction and thus, muscle contraction. Calponin, caldesmon and tropomyosin inhibit actomyosin interaction and the inhibition is relieved upon …
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Molekulare Charakterisierung eines Tropomodulin-Homologen des Fuchsbandwurms E. multilocularis
… kodierten Faktors mit einem kürzlich isolierten Tropomyosin- Homologen aus E. multilocularis nachgewiesen. Basierend auf diesen Daten ist es nun möglich, die biologische Signifikanz der Interaktion von Elp mit EmTY weiterführend zu untersuchen. Sollte sich in diesen Studien herausstellen, daß der …
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Regulation of Mical Redox Post-Translationally-Driven F-Actin Cytoskeletal Dynamics
… screen, I identified two proteins - cofilin and tropomyosin - that modulate Mical-mediated F-actin disassembly. Further investigation revealed that Mical synergizes with cofilin to rapidly and efficiently dismantle F-actin in a redox regulated manner and that this synergism is also necessary and …
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Biochemical effects of organophosphorous compounds on cultured rat and human cardiomyocyte-like cells
… muscle markers such as troponin 1, tropomyosin and α-actin. OP-induced cytotoxicity was assessed by monitoring MTT reduction, LDH release, and caspase-3 activity. Cell death was not observed in mitotic or differentiated H9c2 cells with diazinon, diazoxon, or chlorpyrifos oxon (48 h …
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A study on the modulation of alpha-actinin and filamentous actin
… that α-actinin-4 accomplishes this by inhibiting tropomyosin dependent stabilization of filamentous actin, and maintains a dynamic basal actin array through promoting actin disassembly. Lastly I demonstrate that α-actinin-4 localization in cells can be modulated by phosphorylation of its …
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Einfluß verschiedener Vordehnungen auf isometrische Kraftentwicklung und myofibrilläre ATPase-Aktivität : eine Untersuchung am gehäuteten Streifenpräparat des terminal insuffizienten menschlichen Herzens
… Proteine Aktin, Myosin, Troponin und Tropomyosin im Verbund übrig bleiben. Aktivierungsreihen mit calciumhaltigen Aktivierungslösungen, welche ein gekoppeltes Enzymsystem enthalten. Simultane Messung isometrischer Kraft und myofibrillärer ATPase-Aktivität. Das Verhältnis von …
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The effect of calcium activated protease (CAF) and cathepsin D on bovine muscle myofibrils under varying conditions of pH and temperature
… had degraded titin and, at least partly, tropomyosin, and troponins T and I. A trio of bands in the 30,000-dalton region appeared in the gels of the sedimented myofibrils. CAF treatment caused a significant increase in myofibril fragmentation;Lowered incubation pH or temperature reduced …
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Engineered Cytoskeletal Arrays Reveal Mechanisms Of Membrane Transport And Tubulation
… is inhibited by the presence of nonmuscle tropomyosin Tm2 at the actin intersection. This suggests that tropomyosin may regulate Myo1c tethering of kinesin-1-driven cargo within cells by preventing termination of motility until reaching the highly dynamic actin just beneath the plasma …
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Effects of Actin-Myosin Kinetics on the Calcium Sensitivity of Regulated Thin Filaments
… of thin filaments in striated muscle occurs when tropomyosin exposes myosin binding sites on actin either through calcium-troponin binding or by actin-myosin (AM) strong binding. However, the extent to which each of these binding events contributes to thin filament activation remains unclear. Here …
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Contribution of troponin-I to the regulation of cardiac muscle
… makes up the troponin complex, which along with tropomyosin regulates striated muscle contraction. The cardiac isoform of TnI (cTnI) has a ~30 residue N-terminal extension, which contains two serines (Ser22/23) that become phosphorylated by protein kinase A upon \(\beta\)-adrenergic stimulation. …
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Functional Effects of Calcium Regulation of Thin Filaments at Single Particle Resolution
… the thin filament is composed by troponin (Tn), tropomyosin (Tm), and F-actin. It provides Ca2+-dependent regulation of contraction by modulating myosin attachment and force generation in a cooperative scheme. However, this mechanism remains unclear. To understand thin filament activation, we …
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THE EFFECT OF SKELETAL MUSCLE-SYNTHESIZED BDNF ON MOTOR NEURON SOMA SIZE AND BDNF PROTEIN ABUNDANCE
… synapse with muscle fibers. BDNF transmission to tropomyosin receptor kinase B (TrkB) at the motor neuron axon terminal causes clathrin mediated endocytosis of the complex and an intracellular signaling cascade that recruits dynein motor proteins for retrograde transport of the BDNF/TrkB endosome …
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The exploitation of neuronal survival factors in Burkitt’s lymphoma and germinal centre B cells
… and their receptors: the selective high affinity Tropomyosin receptor kinases TrkA, TrkB and TrkC and the (common) low affinity, tumour necrosis factor receptor member, p75NTR. FDC-like cell lines were also analysed for neurotrophin component expression. This was to question FDCs as potential …
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Actin Cable Function and Regulation in the Budding Yeast, Saccharomyces cerevisiae
… I examined the role of type II myosin and a tropomyosin isoform in retrograde actin flow (Chapter II). In yeast and other cell types, actin undergoes retrograde or centripetal movement from the cell cortex towards the interior of the cell. Retrograde actin flow drives intracellular and …
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