Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"trophozoite"”.
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Giardia trophozoite-secreted proteins and their effects on intestinal epithelia
… from healthy, viable and human infective Giardia trophozoites was shown to be able to mediate profound changes in the physiology of human derived enteric cells, consistent with the production of secreted virulence factors by the parasite. Quantitative proteomic analysis was successfully applied to …
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Probing the chemical biology involved in the haem detoxification pathway of the human malaria parasite, plasmodium falciparum
… the growth of the lumen and uptake over the trophozoite to schizont life stages. It was found that in both NF54 and Dd2 the lumen grew following a Gompertz growth curve during the trophozoite phase, and then abruptly collapsed during the schizont stage. The uptake into the DV followed a …
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Acanthamoeba spp. Secrete a Mannose-Induced Protein that Correlates with Ability to Cause Acanthamoeba Keratitis
… ubiquitously distributed in the environment. The trophozoite form can infect the cornea and cause sight-threatening corneal inflammation known as Acanthamoeba keratitis. The pathogenic cascade of Acanthamoeba keratitis begins when Acanthamoebae bind to mannose expressed on traumatized corneas. …
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Composition and Dynamics of the Plasma Membrane of Entamoeba Histolytica
… simple. It consists of only two main stages, trophozoite and cyst, and transition forms (fig 1). The amoeboid trophozoite is the form of the parasite responsible for the pathogenesis of the disease. It is an anaerobic organism, capable of survival only within the host. It is readily destroyed …
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The Unconventional Amino Acid Starvation Response of the Malaria Parasite, Plasmodium falciparum
… of steady progression through the ring, trophozoite, and schizont stages, followed by subsequent rounds of re-invasion and gradual expansion of the culture, continues at a reduced rate. However, when subjected to isoleucine starvation, parasites progress only through the trophozoite …
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Expression Of Gal/galnac Lectin Of Entamoeba Histolytica In Transgenic Chloroplasts To Develop A Vaccine For Amebiasis
… excysting in the small intestine to form the trophozoite, the parasite adheres to the colonic mucus and epithelial cells through interaction of Gal/GalNAc lectin, an amebic surface adhesin with the host glycoconjugates. The parasite then secrets the proteolytic enzymes that disrupt the …
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Systematic Survey of the Plasmodium falciparum Hemoglobin Catabolic Pathway in Intact Cells Using the Digestomics Approach
… and maximal peptide relative abundances at the trophozoite stage. This was associated with differences in peptide cleavage sites and peptide length indicative of changes in proteolytic activity. I also observed significant differences between the intracellular and extracellular Hb peptidomes, …
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Acanthamoebal surface properties and the modulation of phagocytosis
The surface nature of Acanthamoeba trophozoites and cysts was investigated with respect to cell surface charge, hydrophobicity and surface carbohydrate composition. Particulate microelectrophoresis revealed a marked negative charge for both morphological forms, though less for cyst surfaces. …
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Role of the Nfa1 Protein in Pathogenic Naegleria fowleri Co-cultured with Target Cells
… immunolocalization of Nfa1 protein in a trophozoite of N. fowleri. An anti-Nfa1 antibody showed a neutralizing effect on the cytotoxicity of N. fowleri trophozoites against CHO cells, much like treating an anti-Nfa1 antibody on a cocultivating system. In spite of the wide use of N. …
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Protein coding transcription and control of vsp gene expression in the protozoan parasite Giardia lamblia
… selective activity against G. lamblia. Giardia trophozoites exhibit antigenic variation of a surface protein encoded by a family of genes known as the vsp genes. A single trophozoite expresses only one vsp at a time and it has been previously determined that steady state mRNA of only the …
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Pharmacological effect of MEK inhibitors on plasmodium falciparum
… 24 hours of the asexual cycle, but during the trophozoite stage. Taken together, our data strongly suggest that infection with P. falciparum modulates the MAP kinase pathway in its host erythrocyte, and that the parasite relies on this pathway for its own survival.
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The Characterization of Circular Extrachromosomal rDNA Elements in Naegleria SPP
… and soil. The Naegleria life stages include: the trophozoite stage, a growth and replication stage characterized by pseudopodic movement, the cyst stage, a hibernation stage characterized a double-walled membrane, and the flagellate stage, a mobility stage that is characterized by the presence of …
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In vitro screening of Trametes versicolor and Acacia nilotica derivatives for antimalarial efficacy
… the compounds for 48- and 72-hour incubations at trophozoite and ring stage. The specific doses for exposure were concentrations of 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml and 200 μg/ml, for each extracted compound. MTT assays were performed alongside parasite IC50 studies in order to define the …
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Establishing an ex vivo model of acanthamoeba keratitis and investigating the phenotypic similarities between the protozoan acanthamoeba and human macrophages
… in tap water and soil with two life stages: the trophozoite and cyst. Acanthamoeba species are opportunistic pathogens of humans that cause two main diseases including a potentially blinding infection of the cornea called Acanthamoeba keratitis (AK) in immunocompetent individuals, and a fatal …
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In vitro modelling of cellular haemozoin and inhibition by β-haematin inhibitors and their derivatives
… of haemoglobin were observed between the early trophozoite to late trophozoite stage.
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Developing novel therapeutic agents for Acanthamoeba infection and investigating the process of encystment
… by which Acanthamoeba may distinguish between trophozoite, cyst and the newly identified lifecycle known as protocyst. The current study has tested 56 novel and existing therapeutic agents for their activity against Acanthamoeba spp. and their toxicity against a human epithelial cell line. The …
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Lead optimisation of dehydroemetine as an antimalarial option through drug repositioning and molecular modelling
… both compounds showed more activity against the trophozoite erythrocytic stages of the parasite. The IC50 speed assays carried out to verify onset of antimalarial activity in emetine and the dehydroemetine diastereomers, found that although the compounds started activity within 24 hours, the IC50 …
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A metallomic, proteomic and lipidomic investigation of the malaria parasites digestive vacuole and insights into the mediators of haemozoin formation
… haemoglobin having been ingested at this mature trophozoite stage and the active conversion of free haem (Fe(III)PPIX) into biocrystalline haemozoin. All other trace metals exhibited 2 to 4-fold increases in metal ion content as the parasite matured and were detected in amounts higher than those …