Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"trophectoderm"”.
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Equine Trophectoderm Cells and Their Role in Fetal-Maternal Recognition
… recognition. Six days after fertilization, the trophectoderm (TE), a placenta precursor is formed. Signals emanating from the TE to the uterine environment are critical to maternal recognition of pregnancy. The identity of factors necessary for this process remain unknown. A novel equine induced …
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Conversion of equine umbilical cord matrix mesenchymal stem cells to the trophectoderm lineage using the Yamanaka reprogramming factors
… express the ES marker NANOG, but did express the trophectoderm markers CDX2 and TFAP2A. Both OCT4 and CDX2 were colocalized to the nucleus. The transduced equine cells were termed equine induced trophoblast (iTr) cells. Culture of the iTr cell in suspension resulted in formation of blastocyst-like …
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Biological role of conceptus derived factors during early pregnancy in ruminants
… or asynchrony between the conceptus (embryo and trophectoderm) and uterus. During early pregnancy in sheep, endometrial functions are primarily regulated by ovarian progesterone (P4) and interferon tau (IFNT) from the conceptus trophectoderm. In addition to IFNT, the conceptus as well as the …
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Trophoblast specification and differentiation potential in early human and non-human primate embryogenesis
… trophoblast populations: preimplantation trophectoderm, postimplantation luminal cytotrophoblast, and postimplantation syncytiotrophoblast. Marmoset trophectoderm differentiated into syncytiotrophoblast upon implantation but did not form invasive extravillous trophoblast. Staining of in …
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The Influence of Preovulatory Estradiol on Uterine Transcriptomics and Proteomics Around Maternal Recognition of Pregnancy in Beef Cattle
… preovulatory estradiol exposure on uterine and trophectoderm transcriptomes, and uterine luminal fluid (ULF) protein composition. Beef cows/heifers were synchronized, artificially inseminated (d 0), and grouped into either high (highE2) or low (lowE2) preovulatory estradiol. Uteri were flushed …
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A Study of Mouse Peri-Implantation Morphogenesis
… and the two extraembryonic lineages, the trophectoderm and primitive endoderm. My first goal was to characterise the blastocyst to egg cylinder transition in mouse embryos and understand its mechanism through live imaging and inhibitor treatments (1). Then, I aimed to examine how the …
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Bayesian level sets and texture models for image segmentation and classification with application to non-invasive stem cell monitoring
… and segments images of pluripotent hESC and trophectoderm colonies with 97% and 91% accuracy for high-content screening applications and requires no previous human initialization. While no prior knowledge of colony class is assumed, the framework allows for its incorporation. The BLS is also …
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Development of an Interferon Bioassay and Primitive Endoderm Cell Lines to Study Lineage Specification During Early Bovine Embryogenesis
… are the first two lineage specification events, trophectoderm (TE) and primitive endoderm (PrE) specification, occurring between days 6 and 8 of gestation. The TE is responsible for uterine attachment and production of interferon-tau (IFNT), the factor of maternal recognition of pregnancy in …
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Investigating the consequences of chromosome abnormalities arising during pre-implantation development of the mouse
… higher rates of apoptosis, while in the trophectoderm (TE) lineage effects on the cell-cycle predominated. Depletion continued throughout post-implantation development. Significantly, the presence of a critical number of control blastomeres within the embryo could rescue the early …
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Regulation of amnion induction during the human formative pluripotency transition
… resembles the pre-implantation epiblast, retain trophectoderm (TE) potential, but are resistant to somatic lineages induction. Primed hPSC is related to post-implantation epiblast and readily form germ layers but lack TE potential. The formative transition, corresponding to the peri-implantation …
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Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
… NT blastocysts, both in the inner cell mass and trophectoderm. Furthermore, G₀ donors significantly improved development into cloned blastocysts. In conclusion, quiescence induced long-term epigenetic changes, specifically H3K9me3 hypomethylation, that correlated with increased donor cell …
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Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence.
… as evident by increased blastocyst total and trophectoderm cell numbers. These results were further verified in neutralization experiments of the exogenous growth factors and of the native OSFs. Follistatin and the kinase inhibitor SB-431542, which antagonize BMP-15 and GDF-9, respectively, …
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The role of polarisation in the first cell fate decision of the mouse embryo
… inner cell mass (ICM) from extra-embryonic trophectoderm (TE) during pre- implantation development. In the mouse, the process is classically attributed to the consequences of apical-basal polarity, which forms at the 8-cell stage: those cells which retain the apical domain after cell …
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Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… cell mass (ICM) cell number without affecting trophectoderm (TE) cell number. Additionally, we found that IL6 activated signal transducer and activator of transcription 3 (STAT3) specifically within ICM cells. LIF, however, did not affect ICM cell number or activate STAT3 in ICM cells, and was …
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Post-Transfer Outcomes in Cultured Bovine Embryos Supplemented with Epidermal Growth Factor, Fibroblast Growth Factor 2, and Insulin-Like Growth Factor 1
… in vitro derived bovine embryo development and trophectoderm cell numbers. The objectives of this work were to determine if EFI treatment during in vitro bovine embryo culture improves transferable embryo quality and post-transfer placental and fetal development. For each replicate (3 total), …
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Investigating the Actions of Interleukin-6 Family Members on Post-Hatching Blastocyst Development in Cattle
… cell mass (ICM) cell number but did not affect trophectoderm (TE) cell numbers. IL6 also increased the ICM:TE ratio before and after cryopreservation and ICM cell number after freezing. All IL6 doses reduced the number of cells undergoing apoptosis in the TE, but not in the ICM. In the second …
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Morphogenesis of the early post-implantation mouse embryo
… in the tissue. The ExE originates in the polar trophectoderm, a monolayer covering the epiblast of the blastocyst, which expands and undergoes invagination to form a slit-like cavity. By carrying out analyses of fixed specimens and live imaging of cultured embryos, I have found that the epiblast …
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Mechanisms behind the fate of early chromosomal and transcriptional heterogeneities in the mouse embryo
… and the outer layer of the extra-embryonic trophectoderm (TE). This organization prepares the embryo for implantation and subsequent development. This study aims to explore two broad questions: 1) what mechanisms dictate the fate of the progenies of the chromosomally abnormal cells generated …
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Role of Histone Lysine Demethylase, KDM1B, in Trophoblast Stem Cell Self-Renewal and Differentiation
… the blastocyst stage with the emergence of the trophectoderm (TE) and the inner cell mass (ICM). The TE is the precursor population of all major placental cell types. Reflecting this developmental plasticity, trophoblast stem cells (TSCs) can be derived from the TE of mouse blastocysts. TSCs …
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Dietary Supplementation of Omega-3 Fatty Acids Influences the Equine Maternal Uterine Environment and Embryonic Development
… along with GATA3 and ELF3, markers of trophectoderm differentiation were greater (P< 0.05) in embryos from LCPUFA supplemented mares (n=5), than controls (n=5). These results indicate that algae-derived LCPUFA supplementation during the peri-conceptual period alters the post-ovulatory …
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