Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"tricarboxylic acid (TCA)"”.
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Quantifying the Intracellular Metabolic Network that Establishes the Simultaneous Utilization of Sugars and Aromatic Substrates in Pseudomonas putida KT2440
… sugar (glucose) and an aromatic carboxylic acid (benzoate). Through a combination of 13C tracer experiments with metabolic flux analysis (MFA), I elucidated and quantified the discriminate metabolic routing of each substrate throughout central carbon metabolism. The results determined that …
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Towards Artificial Photosynthesis: Yeast-Inorganic Hybrid System
… drives carboxylation of the five-carbon tricarboxylic acid (TCA) cycle metabolite alphaketoglutarate to form the six-carbon metabolite citrate. This incorporation of CO2 into yeast biomass allows for more carbon efficient ethanol production and illustrates how an engineered light-driven …
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The Mitochondrial Fusion Protein, Optic Atrophy 1 (OPA1), Alters Cellular Metabolism to Support Cancer Cell Viability
… and thereby further coordinates with the tricarboxylic acid (TCA) cycle. To study mitochondrial fusion as a target, we found that silencing of OPA1 in TNBC models led to suppressed growth in anchorage-independent soft agar and tumour xenograft experiments. OPA1 silencing disrupted cristae …
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Defective branched chain amino acid catabolism impairs exercise capacity and glucose homeostasis in the mouse
The branched-chain amino acids (BCAAs) leucine, isoleucine and valine, play a central role in substrate metabolism, energetics, and protein synthesis. Although BCAA supplementation is widely used to improve exercise capacity and physical fitness, elevated levels of the BCAAs have been implicated in …
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Interrogating and potentiating energy metabolism in the human brain after traumatic brain injury
… the brain to interrogate glucose, lactate and tricarboxylic acid (TCA) cycle metabolism. Study I: I defined the optimal parameters for quantitative proton and carbon-13 NMR of cerebral microdialysates. Study II: I measured baseline microdialysate metabolite concentrations for brain and muscle …
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Enzymology and Physiology of a New Type of Phosphoenolpyruvate Carboxylase and the Development of a Pyruvate Carboxylase Expression System
… in studying the junction of glycolysis and the tricarboxylic acid (TCA) cycle, specifically the enzymes phosphoenolpyruvate carboxykinase, pyruvate carboxylase and phosphoenolpyruvate carboxylase. All produce oxaloacetate (OAA) for the cell. OAA production is critical for cell carbon synthesis …
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The Role of Pyruvate Dehydrogenase in Cell Growth
… gates entry of glucose derived carbon into the tricarboxylic acid (TCA) cycle. To test whether cells require glucose oxidation for proliferation, the work in this thesis establishes a model wherein PDH activity is suppressed using RNA interference. Small hairpin RNAs against the transcript …
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The growth, physiology and intermediary metabolism of bacillus stearothermophilus.
… this work were to determine whether a functional tricarboxylic acid (TCA) cycle was present in vegetative cells of B. stearothermophilus, and to understand the effects of growth conditions on the carbohydrate metabolism and cytochrome content of the organism. In order to study the intermediary …
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Investigation of metabolism in glioblastoma patient-derived xenografts using deuterium metabolic imaging
… complex 1 resulted in a reduction in tricarboxylic acid (TCA) cycle activity and a compensatory increase in glycolysis, and that the mitochondrial subtype is more sensitive to this treatment. Moreover, 2H CSI could be used to image these metabolic changes in vivo. Finally, I explored …
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Glutamine Antagonism and Its Utlity [sic] as a Therapeutic Modality in Cancer
… is important in cancer as it fuels the TCA cycle, plays a role in redox homeostasis, and contributes to the production of nucleotides, amino acids, and lipids for survival, making glutamine metabolism a promising target in cancer therapy. The work outlined in this dissertation focuses on …
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Metabolic flux, transport activity, and subcellular communication in intact hearts from dynamic ¹³C NMR spectroscopy
… butyrate, and with or without KCl arrest. The tricarboxylic acid (TCA) cycle flux and the interconversion rate between (-ketoglutarate and glutamate were determined by least-square fitting of a kinetic model to NMR data. Rates were compared to flux through glutamate-oxaloacetate transaminase …
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Functional and mathematical analysis of the glyoxylate shunt in Streptomyces coelicolor
… pathway in S. coelicolor during growth on fatty acids and therefore an important pathway in providing precursors for secondary metabolism. The S. coelicolor genome contains genes for a second anapleurotic pathway, the ethylmalonyl-CoA pathway. The relative importance of both to anapleurosis in …
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The role of ABHD11 in the regulation of the hypoxia inducible transcription factors
… These enzymes all require oxygen, iron and the Tricarboxylic Acid (TCA) cycle metabolite, 2-oxoglutarate (2-OG/a-ketoglutarate) for catalytic activity. Therefore, understanding how 2-OG levels are regulated is essential to dissect the relative contribution of metabolism to activation of HIFs. A …
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Studies on lipoic acid metabolism
The covalent attachment of lipoic acid to the lipoyl domains (LDs) of the central metabolism enzymes pyruvate dehydrogenase (PDH) and oxoglutarate dehydrogenase (OGDH) is essential for their activation and thus for respiratory growth in the bacterium Escherichia coli and the yeast Saccharomyces …
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Metabolic flux analysis of Escherichia coli MG1655 under octanoic acid (C8) stress
… chemicals produced renewably. Carboxylic acids [e.g., short chain fatty acids (SCFAs) such as octanoic acid (C8)] are such chemical intermediates that can be produced by Escherichia coli engineered with thioesterases specific for short chain fatty acids. However, C8 is toxic to E. coli at …
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The role of metabolism in the pathogenesis of adherent invasive Escherichia coli (AIEC)
… a systematic mutational approach, that while the Tricarboxylic acid (TCA) cycle, the glyoxylate pathway, the Entner-Doudoroff (ED) pathway, the Pentose Phosphate (PP) pathway and gluconeogenesis are dispensable for the intramacrophagic growth of HM605, glycolysis is an absolute requirement for the …
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Finding Typhoid Mary: Identifying Latent Carriers of Salmonella enterica serovar Typhimurium
… By activating human monocytes with retinoic acid and vitamin D3, we were able to isolate Salmonella from such cells 45 days after inoculation. We subsequently used this model to identify genes that were upregulated in this chronic infection model. We found that aceA, a gene that codes for …
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Impaired ketogenesis is implicated in intestinal stem cell function and epithelial regeneration in ulcerative colitis
… pathways in ISCs, with de-regulation of fatty acid metabolism pathways, particularly ketogenesis, with HMGCS2 as the top downregulated gene in UC ISCs. HMGCS2, the rate limiting mitochondrial matrix enzyme in the ketogenesis pathway, producing βHB, which alongside providing metabolites for the …
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ENVIRONMENTAL BENEFIT AND BURDEN OF POSTBIOTICS IN ANIMAL NUTRITION
… Additionally, the increased levels of tricarboxylic acid (TCA) cycle interme-diates (2-oxoglutarate and citrate) in untargeted metabolomic treated cecal samples may point to a slight shift in the bacterial community, which metagenomic approaches might be able to explain in the future. …
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The role of NAD+ compartmentation and dynamics in Arabidopsis thaliana
… associated with the induction of abscisic acid (ABA)-responsive genes. Additionally, NAD+ feeding of aba- 2 and ost1 seedlings, impaired in ABA synthesis and ABA signaling, respectively, did not impact on stomatal number, whereas the inhibition of ABA synthesis rescued the stomatal …
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