Global ETD Search
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Showing 1 to 20 of 80 for “"transposons"”.
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Bacteroides Conjugative Transposons: Ecology and Mechanisms
CTnDOT is a 65 kb Bacteroides CTn that carries genes encoding for tetracycline resistance and erythromycin resistance. Previous studies had shown that the attDOT recombination site and integrase of CTnDOT (IntDOT) are sufficient and essential for integration in vivo. Even though the integrase of …
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Activities of Bacteroides Conjugative Transposons: CTnGERM1 and CTnDOT
CTnDOT is a conjugative transposon (CTn) that is found in many Bacteroides strains. Transfer of CTnDOT is stimulated 100--1000 fold if the cells are exposed to tetracycline (Tc). An operon that contains a Tc resistance gene, tetQ, and two regulatory genes, rteA and rteB, is essential for control of …
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Integration and Excision Mechanism of Bacteroides Conjugative Transposons
The third project is about the regulatory mechanism of RteB on CTnDOT. RteA and RteB are shown to be global regulators that regulate many genes on Bacteroides host. Whether RteB regulates CTnDOT and NBU1 excision directly or not is investigated in this study. Both RteB and the mutant are …
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Characterization and Regulation of Bacteroides Mobilizable Transposons, NBUs
Bacteroides MTns forms a heterogeneous group of elements with conserved functional regions. But the conserved region on different elements is different. This raises a possibility that Bacteroides MTns are modularly assembled. This speculation is supported by partial sequencing of NBU3. The sequence …
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Characterization and Engineering of Transposons for Genome Editing
Since the human genome was sequenced in 2000, we have rapidly elucidated previously unappreciated associations between our genetic code and human disease. Driven by the rapid development of new molecular technologies, there has been increased desire to treat these genetically rooted diseases by …
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Characterisation of trimethoprim resistance transposons and their gene products
… a similar evolutionary origin for the two transposons and reiterates the potential of trimethoprim resistance transfer between animal and human resevoirs
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Development of tools to study the association of transposons to agronomic traits
Transposable elements (Transposons; TEs) constitute the majority of DNA in genomes and are a major source of genetic polymorphisms. TEs act as potential regulators of gene expression and lead to phenotypic plasticity in plants and animals. In crops, several TEs were identified to influence alleles …
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Construction and Use of a System for Studying the Integration of Bacteroides Conjugative Transposons
… about the way in which Bacteroides conjugative transposons integrate into their chromosomal target sites, or what the nature of these sites are. In the past, this question has been very difficult to address due to the large size of the Bacteroides conjugative transposons. In this study, a …
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Characterization of a novel tetracycline resistance gene from the Bacteroides transposons Tn4351 and Tn4400
… that was originally found on two Bactetoides transposons, Tn4351 and Tn4400, confers resistance on Escherichia coli, but only when the cells are grown aerobically. I first showed that the product of the $\rm \sp{*}Tc\sp{r}$ gene is a 44 kDa cytoplasmic protein. This indicated that the …
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Identification of genes in Sinorhizobium meliloti relevant to symbiosis and competitiveness using signature tagged transposons
… a strategy based on libraries of tag-carrying transposons can be used. In the transposon-based STM, the number of mutants that can be pooled together in one experiment depends on the quantity of transposons containing different tags. In this study, the construction of a novel set of 412 …
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Interactions between the Bacteroides conjugative transposons and the plasmids and insertion elements (NBUs) they mobilize
… A family of chromosomally located conjugative transposons is responsible for the spread of tetracycline resistance in the Bacteroides group and is also contributing to the spread of resistance to other antibiotics. Bacteroides conjugative transposons not only transfer themselves but also …
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The DNA Transfer Region of Bacteroides Conjugative Transposons Tc(r)Em(r)DOT and Tc(r)ERL
… transfer gene sequences of several conjugative transposons. Two of the newly discovered transfer genes possess sequence homology with genes from other transfer systems and the significance of this relationship is discussed. Additionally, the first nucleotide and amino acid level homology between …
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Análise comparativa de transposons classe II (CACTA e Mutator) nos genomas de Vigna unguiculada, Phaseolus vulgaris e Medicago truncatula
… esse trabalho identificou um total de 5,64 Mb de transposons e 48,42 Mb de retrotransposons, distribuídos em todos os cromossomos de P. vulgaris; 14,04 Mb de transposons e 51,20 de retrotransposons em M. truncatula, e 2,38 Mb e 9,34 Mb de transposons e retrotransposons, respectivamente, em V. …
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DD34E DNA Transposable Elements of Mosquitoes: Whole-Genome Survey, Evolution, and Transposition
… mosquito, Anopheles gambiae. Twenty-six new transposons as well as a new family, gambol, were identified. The gambol family shares the DD34E catalytic motif with Tc1-DD34E transposons, but is distinct from these elements in their phylogenetic relationships. Although gambol appears to be …
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Naujų Mu transpozonų konstravimas ir jų panaudojimas baltymų struktūros tyrimuose /
Transposons have been widely used in experiments of genome analyzing and directional rearrangements in vivo, and lately in vitro, since they had been discovered. In this work Tn-Km transposon was modified providing unique targets of restriction endonucleases MreI, Bsp1407I, BseXI, AarI within …
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The Role of Bacteroides Conjugative Transposons in the Spread of Antibiotic Resistance Genes, And, The Role of recA in DNA Damage Repair in Deincoccus Radiodurans
The work presented in this thesis covers two unrelated projects. The first project involves using Bacteroides species, which are normal residents of the human colon, as a model for investigating the horizontal transmission of antibiotic resistance genes in the environment. The data presented in …
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Transposable elements: What have you done for me lately? A genomics based investigation into the potential functional roles of transposable elements using the model organism Caenorhabditis elegans
… present as disperse repetitive elements called transposons. Transposons have the unique ability to move to new chromosomal locations. Problems of uncontrolled movement of transposons can result in mutations, rearrangement, and even broken chromosomes. Often termed "selfish parasites" that invade …
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Development of a Genetic Modification System in <i>Clostridium scatologenes</i> ATCC 25775 for Generation of Mutants
… scatologenes</i> ATCC 25775. Bacteriophages and transposons are useful tools to achieve this goal. Isolation of <i>Clostridium scatologenes</i> ATCC 25775 bacteriophage was attempted by prophage induction and enrichments using environmental sources. To induce prophages, cultures of <i>Clostridium …
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piRNA-dependent transcriptional gene silencing during Drosophila oogenesis and embryogenesis
… animal gonads from the deleterious effects of transposons, thus maintaining transgenerational genome integrity. In Drosophila melanogaster ovaries, piRNA-Piwi complexes localise to the nucleus and scan nascent transcripts for transposon expression by using complementary antisense piRNAs as …
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Mechanisms governing transposable element expression in the Drosophila germ line
… damage the genome and disrupt gene expression. Transposons are known to be particularly active in the developmental context of the germ line, to fulfil their selfish drive to increase in copy number in the genome that will be passed to the next generation. Due to this threat, dedicated …
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