Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"transposon sequencing"”.
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Highly Saturated Transposon Sequencing identifies genes impacting Staphylococcus aureus pathogenesis in macrophages
… vitro transposition method to produce saturated transposon mutant libraries in S. aureus, and performed Tn-Seq to identify candidate genes with significantly altered abundance following macrophage invasion. While some significant genes were strain-specific, 107 were identified in common across …
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Population Sequencing for Studying Natural and Artifcial Variation in C. elegans
<p>The advent of high coverage and low cost sequencing technologies has allowed for</p><p>newer and more powerful approaches in molecular and population genetics. Transposon</p><p>sequencing, where genome-saturated mutant populations allele frequencies are</p><p>measured before and after selection, …
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Genetic Determinants of Salmonella and Campylobacter Required for In Vitro Fitness
… For the purpose, we used a high throughput Transposon sequencing (Tn-seq) that utilizes next generation sequencing (NGS) to screen hundreds of thousands of mutants simultaneously. In Chapter 1, we reviewed the technical aspects of different Tn-seq methods along with their pros and cons and …
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Transposon Mutagenesis Facilitates Discovery Of Genotype-Phenotype Associations and Functional Interrogation of the <i>Mycobacterium Kansasii</i> Genome
… demonstrates the functionality of a phage-based transposon (Tn) mutagenesis system in <em>Mk </em>by generating and screening a library of random insertion mutants for altered macrocolony morphology. In this screen, we identified 41 mutants exhibiting unique phenotypes, among them one carrying …
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Characterization of proteins involved in Bacillus subtilis spore formation and germination
… high-throughput genetic screening method called transposon sequencing was used. This analysis identified genes that had not been previously implicated in germination. To investigate their functions, a number of functional assays of all the Ger mutant strains were performed that indicated a delay …