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Showing 1 to 15 of 15 for “"transposon mutant"”.
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Analysis of a Tn917 Transposon Mutant and Preliminary Characterization of NonHemolytic, Catalase-Deficient Variants of Staphylococcus aureus
Crum, Russell M., Analysis of a Tn917 Transposon Mutant and Preliminary Characterization of Nonhemolytic, Catalase-Deficient Variants of Staphylococcus aureus. Masters of Science (Microbiology). June 1999. Pages-101. Tables-15. Figures-10. A Tn917 transposon mutant of Staphylococcus aureus S6C was …
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A streamlined Workflow for Transposon Mutagenesis in Corynebacterium glutamicum Based on Third-Generation Sequencing and its Application for Advanced Genetic Screens
… CR101 for an efficient integration system, a transposon mutagenesis system based on the endogenous IS*Cg1* transposase and a streamlined workflow for transposon mutant library preparation and sequencing using Oxford Nanopore Technologies (ONT) nanopore Sequencing. Mobile genetic elements …
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Regulating rsmA Expression in Pseudomonas aeruginosa
… of<em> rsmA</em> is not well elucidated. Transposon mutagenesis was performed on P. aeruginosa containing a transcriptional <em>rsmA-lacZ</em> fusion to answer this question. Mutants were screened via β-galactosidase assay and transposon insertions identified via arbitrary PCR. A probable …
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Optimization of an Immune Assay to Detect Binding of NOD2 to Staphylococcus Aureus
… type and two bacterial strains from the Nebraska Transposon Mutant Library (NTML), murine NOD2 transgenic cells, and two detection systems. I hypothesized that both mutant strains would have differential binding to NOD2 compared to the wild-type strain. Results showed that only one mutant strain, …
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Highly Saturated Transposon Sequencing identifies genes impacting Staphylococcus aureus pathogenesis in macrophages
… vitro transposition method to produce saturated transposon mutant libraries in S. aureus, and performed Tn-Seq to identify candidate genes with significantly altered abundance following macrophage invasion. While some significant genes were strain-specific, 107 were identified in common across …
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Genome scale metabolic modelling to identify metabolic responses of resistant bacteria to antibiotic challenges
… drug targets. Experimental data from transposon mutant libraries grown in presence of tilmicosin, a macrolide antibiotic was integrated into GSM analyses. The GSMs of two strains of E. coli, including a novel clinical strain were constructed and analysed. A potential pathway involved …
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Molecular characterization of a gene that is associated with Listeria monocytogenes serotype 4b-specific surface antigen expression
… expression of L. monocytogenes serotype 4b. One mutant, 27B6, derived from L.monocytogenes serotype 4b strain 2381L (Jalisco cheese outbreak), was found to be negative in immunoblot reactions with all three serotype 4b-specific MAbs C74.22, C74.33, and C74.180. Chromatographic analysis showed …
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Understanding the intrinsic antibiotic resistance in Lysobacter enzymogenes, a ubiquitous environmental bacterium
… LeC3 and LaATCC29479. Second, screening two Tn5 transposon mutant libraries of LeC3 recovered 12 and 19 mutants with decreased ampicillin and spectinomycin resistance, respectively. Further characterization of selected mutants showed that cell permeability, β-lactamase activity and transport …
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Niche adaptations of the vibrionaceae, from the coastal ocean to the laboratory
… the algal constituent, alginate. By selecting a transposon-mutant collection for growth on these resources, I find that Apocyclops is a replete resource, whereas Fucus is intermediate to Apocyclops and alginate in its anabolic requirements; that catabolic pathways have redundancy, which anabolic …
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Novel High-throughput Technologies for Applications in Microbiology
… efficiency and facilitates the creation of transposon mutant libraries. Collectively, the high-throughput solutions for microbiology overcome the limitations of cuvette-based electroporation, significantly improving efficiency and consistency in bacterial genetic engineering. These …
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Novel Molecules from Environmental DNA-Derived Type II Polyketide Biosynthetic Gene Clusters
… clone. A biosynthetic analysis of wild type and transposon mutant V167 cultures indicated that erdacin arises from the heterodimerization of two 13-carbon monomer subunits that are both derived from octaketide precursors. These two 13-carbon subunits arise from two different second cyclizations, …
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Identification and characterization of virulence associated factors of C. jejuni
… of host cells by the pathogen, 660 clones of a transposon mutant library generated in the clinical C. jejuni isolate B2 were screened. Thereby, a clone with a transposon insertion in gene cj0952c was identified. As in the sequenced C. jejuni strain NCTC 11168, the respective protein together …
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Erwinia carotovora extracellular proteases: characterization and role in soft rot
… Ecc intracellular proteins. Analysis of deletion mutants indicated a 1.2 kb region necessary for Prt1 production. Sequencing of the pSK1 insert revealed a 1,041 bp open reading frame (ORF1) corresponding to the prtl region. ORF1 encodes a putative polypeptide of 347 amino acids with a total …
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Structural and functional elucidation of four putative PQS-binding proteins in Pseudomonas aeruginosa
… generated and confirmed “clean” *pfpI* deletion mutants for phenotypic and ‘omic analyses. I observed discrepancies in phenotypes between the *pfpI* deletion mutant and the *pfpI* transposon mutants previously reported in the published literature, which I sought to reconcile through subsequent …
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Towards Understanding the Genetic Basis of Virulence in Pseudomonas aeruginosa
… assays using an arrayed P. aeruginosa transposon mutant library covering approximately 4000 non-essential genes. Mutants were phenotypically screened using agar-based assays for six virulence factors that are associated with CF lung infections and are frequently observed to be variable …