Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 259 for “"transposon"”.
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Excision of the Bacteroides Conjugative Transposon CTnDOT
In this study, we showed that expression of the exc operon is regulated by tetracycline and mediated by the regulatory proteins, RteA, RteB, and RteC. Tetracycline induces the expression of rteA and rteB, genes in the tetQ operon. Then, RteB induces the expression of rteC. RteC, subsequently, …
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Regulatory Genes of the Bacteroides Conjugative Transposon, CTnDOT
In order to examine which genes are responsible for excision, I first demonstrated that the excision genes orf2c, orf2d, orf3, and exc are part of an operon (the orf2c operon) and that this operon is regulated at the transcriptional level. I found that RteC is an DNA-binding activator of the orf2c …
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Characterization of the Integrase of the Bacteroides Mobilizable Transposon NBU1
The Bacteroides mobilizable transposon NBU1 uses an integrase (IntN1) for its integration and excision from the host chromosome. IntN1 has six conserved amino acids in the C-terminal end (RKHRHY) that are characteristic of tyrosine recombinases. I used site-directed mutagenesis to construct alanine …
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Responses to transposon activity and genome damage during germline development
… conflicts between selfish genetic elements, like transposons, and the host genome take place. Excessive transposon activity fulfils the selfish drive of transposons to increase in copy number in the host genome but can impair genome integrity and functionality, thereby threatening the faithful …
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The Transposon-Encoded Protein TnpB is an RNA-Guided Nuclease
… its evolutionary origins in TnpB, an abundant transposon-encoded protein that contains a predicted nuclease domain. The biochemical properties of TnpB are unknown, but may provide clues to the evolutionary origins of RNA-guided activity. In this thesis, we investigate the biochemical activity …
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Silencing Through Heterochromatin Formation: The Role of Piwi in Transposon Targeting
… roles in mitosis, telomere homeostasis, and transposon: TE) control, all necessary to ensure genome stability. A precisely controlled mechanism to designate which regions of the genome should be so packaged is critical to the fitness of an organism. Mis-localized heterochromatin can lead to …
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Identifying biosynthetic pathways for mycobacterial cell wall components using transposon mutagenesis
… novel drug targets. A strategy based on random transposon (Tn) mutagenesis with two different screening criteria, altered colony morphology and mycobacteriophage resistance, was developed. Non-pathogenic Mycobacterium smegmatis and the fish pathogen Mycobacterium marinum were used for generating …
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The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT
A critical requirement for studying transposition is the availability of a defined in vitro transposition system. Using the partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. …
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Transformation of a Transposon Construct into Tomato for Functional Genomics Studies
… tomato with a T-DNA construct bearing a transposon from maize that can be used for mutagenesis when it transposes or moves around the genome of the tomato. The vector used is a two-component in-cis Ac-Ds system which needs a single transformation event. It was proved that it worked in …
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A Mosquito DNA Transposon Agh1: Structure, Evolution and Evidence of Activity
… analyses clearly suggest that unlike most DD34E transposons that are similar to the Tc1 family, AGH1 belongs to a different clade that is related to the previously characterized fungal TE Ant and protozoan TEC1 and TEC2. Truncated AGH1 and AGH1-related MITE (Miniature inverted-repeat TE) families …
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Highly Saturated Transposon Sequencing identifies genes impacting Staphylococcus aureus pathogenesis in macrophages
… vitro transposition method to produce saturated transposon mutant libraries in S. aureus, and performed Tn-Seq to identify candidate genes with significantly altered abundance following macrophage invasion. While some significant genes were strain-specific, 107 were identified in common across …
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Transposon Tn5 mutagenesis in Pseudomonas aeruginosa: a study of Beta-lactamase expression
… class C B-lactamase gene of P. aeruginosa. Transposon mutagenesis was considered an effective method for the identification and subsequent cloning of such regulatory genes. To facilitate identification of Tn5 insertions in P. aeruginosa a transposon Tn5-derivative, Tn5::gen, encoding …
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Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT
… elements. One such element is a conjugative transposon (sometimes referred to as an integrative conjugative element, or ICE) called CTnDOT, which is a 65 kb ICE that encodes resistance to the antibiotics erythromycin and tetracycline. A notable feature of CTnDOT is that excision from the …
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Transposon Tagging in Strawberry and Potato and Characterization of Representative Strawberry Mutants
… having large populations of T-DNA insertional or transposon tagged mutants. The idea of using AcDs construct to create transposon tagged mutant populations has also been applied in many species. Here we transformed two species, Fragaria vesca and a monoploid potato, Solanum phureja 1-3-516, which …
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Tetracycline-Associated Regulation of the Transfer Genes of the Bacteroides Conjugative Transposon CTnDOT
… colonic Bacteroides spp. harbor a conjugative transposon (CTn) called CTnDOT. CTnDOT carries two antibiotic resistance genes tetQ and ermF. A distinctive feature of CTnDOT is that both its excision and transfer are stimulated by tetracycline. The regulation of excision has been described …
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Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262
… Clostridium acetobutylicum strain P262, using transposon-induced metronidazole resistance as a selection system. In the process, the feasibility of transposon mutagenesis in this strain, which lacks conventional systems for DNA delivery, was assessed, and the nature of metronidazole …
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Construction and Use of a Transposon for Identification of Essential Genes in Mycobacteria
… new drug targets. In this study, a mariner-based transposon, TnQuoVadis, was constructed to identify essential genes as potential drug targets. This transposon has an outward-facing anhydrotetracycline (ATc)-inducible promoter at each end. A mutant with TnQuoVadis inserted upstream of an essential …
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Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… utilized Agrobacterium transformation and the transposon tagging construct, Ac-DsATag-Bar_gosGFP, to produce activation tagged and knockout mutants in the processing tomato variety, M82. The construct contained hygromycin resistance (hyg), green fluorescent protein (GFP), and maize transposase …
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