Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"transposase"”.
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Carboxyl terminal truncations of the Phage Mu transposase
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Biochemische und funktionelle Untersuchungen der Transposase des Activator-Elements aus Zea mays
… gehört, codiert für ein einzelnes Protein, die Transposase (TPase). Dieses Protein ist in vivo als einziges essentiell für die Transposition von Ac notwendig. Die Expression der TPase in E. coli war bisher nur in unlöslicher Form möglich. Der Nachweis, daß die TPase in vitro an die subterminalen …
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Regulation of Mu transposition via communication between the transposase and Mu-encoded accessory protein
… We explore the interactions between the Mu transposase, MuA, and a Mu-encoded accessory protein, MuB. Together, these two proteins regulate Mu transposition and targeting. We demonstrate that MuB interacts with multiple subunits of the MuA transposase complex to stimulate transposition. …
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INVESTIGATING GENOME PROTECTION AT G-QUADRUPLEXES: STRUCTURAL-FUNCTIONAL ANALYSIS OF S. CEREVISIAE DOMESTICATED TRANSPOSASE VID22
L'instabilità del genoma è una caratteristica distintiva dell'invecchiamento e di varie patologie umane, tra cui il cancro. L'instabilità genomica è provocata da agenti esogeni che danneggiano il DNA e da molteplici fattori endogeni, tra cui le G-tetradi, strutture secondarie non canoniche del …
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Mechanism and consequences of Mu transpososome remodeling by the ClpX chaperone
… complex does not spontaneously release the DNA; transposase remains so stably bound that subsequent replication of the Mu genome is inhibited. To understand how ClpX releases the replication block without destroying the transpososomes, we characterized the mechanism and products of transpososome …
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Dynamics of protein-DNA interactions in a Mu transpososome
… Transposition is mediated by a protein, the transposase, encoded by the transposon. DNA sequence signals at the two ends of the transposon activate assembly of a transpososome: a complex that include multiple copies of transposase plus both transposon ends. Transpososome assembly, in turn, …
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Organization and dynamics of the bacteriophage Mu transpososome
… complex containing multiple, identical transposase subunits. The cleavage and joining reactions are the result of precise manipulation, by the transposase subunits, of the DNA segments surrounding the two transposon ends and the DNA at the new location. The purpose of this work is to …
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Investigating mechanisms of genome folding by single-cell Hi-C
… library processing steps using a commercial Tn5 transposase. My work shows that without significant optimisation the commercial transposase is not compatible with our protocol. Using the protocol, we successfully processed haploid mouse embryonic stem cells (mESCs) and calculated 3D structures of …
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Chemical and Enzymatic Methods to Detect Chemical Modifications in Nucleic Acids
… features. One example is the use of Tn5 transposase in CUT&Tag. Chapter 5 describes the development of a method to covalently recruit Tn5 transposase to specific features in DNA.
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Transposon Tagging in Strawberry and Potato and Characterization of Representative Strawberry Mutants
… strength of petioles. Since the often-used transposase gene was cloned from the monocot species maize, the efficiency of obtaining germinal transposants was many times lower than expected in order to saturate the genome for diploid species. In order to improve the chance of getting unique …
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A streamlined Workflow for Transposon Mutagenesis in Corynebacterium glutamicum Based on Third-Generation Sequencing and its Application for Advanced Genetic Screens
… system based on the endogenous IS*Cg1* transposase and a streamlined workflow for transposon mutant library preparation and sequencing using Oxford Nanopore Technologies (ONT) nanopore Sequencing. Mobile genetic elements (MGEs) destabilize the genetic material of their hosts. While this …
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DD34E DNA Transposable Elements of Mosquitoes: Whole-Genome Survey, Evolution, and Transposition
… the case that AgTango may be a functional transposase, and I sought to test its potential activity in a cell culture-based inter-plasmid transposition assay using the Herves plasmids as a positive control (Arensburger et al., 2005). AgTango constructs were successfully constructed; however, …
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Auto-tethering as a selection mechanism for recognition of multimeric substrates by the AAA+ unfoldase ClpX
… substrate the Mu transpososome. Phage Mu transposase tetramerizes in the cell to form the Mu transpososome, which mediates replicative transposition of the phage. After transposition is completed, the Mu transpososome forms an extremely stable tetramer that needs to be destabilized by CIpX …
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Development of broadly applicable transgenic tools for the transposon mutagenesis of the red flour beetle, Tribolium castaneum
… to various insect species. To drive the transposase gene for transposable element remobilisation, suitable promoters are needed. The broadly conserved thermotolerance factor, Hsp70, is well-characterised in D. melanogaster and its promoter, which is inducible by high temperatures, …
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Gene editing in Aedes aegypti
… (IE1, UbL40, hsp82 and PUb) to express the Mos1 transposase open reading frame in Mos1-mediated transgenesis. Germline transformation efficiency and size of transgenic cluster were not significantly different when using endogenous Ae. aegypti PUb or the commonly used exogenous Drosophila hsp82 …
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Molecular mediators of cardiac-specific enhancer activation
… key regulatory TFs, we used the Assay for Transposase-Accessible Chromatin (ATAC-seq) to map changes in chromatin accessibility and integrated these data with maps of histone modification patterns and gene expression across several stages of embryonic stem cell (ESC) differentiation toward …
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DNA repair proteins Metnase and PARP1 regulate DNA integration
… contains a SET methyltransferase domain and a transposase domain. Metnase promotes efficiency and accuracy of NHEJ and promotes DNA integration. The SET domain dimethylates histone H3K36 at DSB sites, and the transposase domain binds to the human Mariner transposon Hsmar1 terminal inverted …
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Single cell landscape of innate and adaptive immunity in metastatic melanoma treated with immunotherapy
… (222,351 cells; 39 samples), and single nucleus transposase-accessible chromatin sequencing (45,478 cells; 15 samples). We identified marker genes and functional modules associated with ICI response (e.g. cell-adhesion) and resistance (e.g. oxidative phosphorylation). Through single cell study of …
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Leveraging Single-Cell ATAC-Seq for Genomic Language Models and Multimodal Foundation Models
Single-cell Assay for Transposase-Accessible Chromatin using sequencing (scATAC-seq) has emerged as a powerful tool for profiling chromatin accessibility at single-cell resolution. By capturing epigenomic landscapes, scATAC-seq provides critical insights into the regulatory elements that govern …
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Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… green fluorescent protein (GFP), and maize transposase (TPase) on the stable Ac element, along with a 35S enhancer tetramer and glufosinate herbicide resistance (BAR) on the mobile Ds element. An in vitro propagation strategy was used to produce a population of 25 T0 plants from a single …
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