Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"transport assays"”.
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Purification and characterisation of the Escherichia coli multidrug efflux protein MdtM
… the cell by proteins which are integral membrane transporters. Escherichia coli MDR transporter, MdtM, is a previously uncharacterised, 410 amino acid residues protein from the ubiquitous major facilitator superfamily (MFS). This thesis focuses on the purification and characterisation of MdtM. …
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Charakterisierung der initialen Aufnahme des Kofaktors V (NAD) bei Haemophilus influenzae
… ist. Mit Hilfe von verschiedenen Phosphatase-Assays und dem Malat-Enzym-Assay konnten NADP und Nikotinamid-Mononukleotid (NMN) als physiologisch relevante Substrate identifiziert werden. Die biologische Relevanz von e(P4) für die NAD-Aufnahme wurde durch Mutantenanalyse in Wachstumstests und …
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Functional and mechanistic studies of human mitochondrial carriers
… carrier family is the largest family of transporters, with 53 members in humans. They selectively transport a diverse set of substrates across the mitochondrial inner membrane, and are involved in key aspects of mitochondrial metabolism and function. The identification and …
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MFSD7C: A Solute Carrier Linking Heme and Calcium in Mitochondrial Energy Metabolism
… MFSD7B, have been proposed to function as heme transporters, however this hypothesis was challenged by several groups. We now show that MFSD7C localizes to mitochondria where it interacts with the electron transport chain complex. Loss of MFSD7C in various cell lines and primary cells results in …
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Preliminary characterization of VmTPT2, a putative monoterpene indole alkaloid (MIA) transporter from Vinca minor L. (Apocynaceae)
… recent discovery and characterization of an ABC transporter (CrTPT2) that was shown to mobilize catharanthine from its site of biosynthesis in epidermal cells to the leaf surface of Catharanthus roseus. Bioinformatic analysis of transcriptomes from several geographically distant MIA-producing …
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Charakterisierung von SNARE-Proteinen in der Hefe Saccharomyces cerevisiae
… sind in allen Eukaryonten an der Fusion von Transport-Vesikeln mit Zielmembranen beteiligt, wobei sie Komplexe bilden in denen jeweils 3 Q-SNAREs mit einem R-SNARE interagieren. Im ersten Teil der Dissertation wurde über einen Multicopy-Suppressor-Screen versucht, zusätzliche Proteine …
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Deciphering Multidrug Efflux: Substrate Interactions and Transport Mechanisms in PatAB and NorM-VC
Multidrug efflux transporters are ubiquitously expressed in all kingdoms of life. In bacteria, they participate in several innate physiological functions to aid survival and adaptation. Given their crucial roles in conferring antimicrobial resistance (AMR), the understanding of the structural and …
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A Transferrin conjugated nanoemulsion system for brain delivery of antiretroviral therapy
… cells and 293T cells. Cell uptake and drug transport assays were also conducted to investigate the in vitro activity of Tf-NE-DRV. In vitro efficacy studies, ex vivo imaging studies and in vivo biodistribution studies were performed to investigate the brain targeting function of Tf-NE-DRV. …
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Investigating the substrate binding mechanism of the mitochondrial ADP/ATP carrier
… functional complementation, thermostability and transport assays consistently identified five residues (K30, R88, R197, R246 and R287) to be critical for substrate binding. The Ala variants of these residues did not complement growth of an Aac-deficient strain, abolished the …
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Neutral receptors for anion recognition: synthesis, selectivity studies and transport properties
… receptors and efficient anion transmembrane transporters. Two new asymmetric ureidic receptors L1 (1-(1H-indol-7-yl)-3- (quinolin-2-yl)urea) and L2 (1-(quinolin-2-yl)-3-(quinolin-8-yl)urea), shown in Figure 1, have been synthesised and their affinity towards different anions tested in …
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Energetics and mechanisms of substrate transport by the MATE transporter PfMATE
… stays, and increased mortality. Multidrug transporters play a critical role in the multidrug resistance. These efflux pumps can use the energy derived from ATP hydrolysis and/or electrochemical ion gradients to actively remove structurally unrelated drugs, such as antimicrobials and …
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The impact of mouse placental endocrine function on fetal resource allocation and adult offspring health
… that induce maternal insulin resistance and transports the nutrients available to support fetal growth and metabolic demand. Disturbances in placental endocrine function may lead to pregnancy complications such as gestational diabetes mellitus (GDM) and intrauterine growth restriction (IUGR) …
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On the Structure and Function of Multidrug Efflux Pumps
… partner (AcrZ) for the substrate selectivity and transport activity of an efflux pump protein (AcrB). This regulation happens through induced structural changes which have remained unseen so far. Alongside with these results, a nanodisc reconstitution method was experimentally adapted for a …
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Mechanism and energetics of membrane transporters and channels
"Living cells have evolved specialized transport proteins called membrane transporters and channels that catalyze exchange of materials across the cell membrane. Membrane trans- porters couple the active transport of their specific substrates against their electrochemical gradient. On the other …