Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 20 for “"transpeptidase"”.
-
Studies of Mammary Amino Acid Transport
… shown to have relatively high (gamma)-glutamyl transpeptidase activity, and activity increased at the onset of lactation. This increase was shown to be dependent on prolactin. The timing and magnitude of the changes in (gamma)-glutamyl transpeptidase activity during pregnancy, lactation, and …
-
The Development of Certain Enzymes and Iso-Enzymes During Normal Pregnancy
… cystine aminopeptidase, γ-glutamyl transpeptidase, alkaline phosphatase, glucose-6-phosphatase, 5-nucleotidase, n-acetyl-β-glucosaminidase, n-acetyl-β-galactosaminidase and β-glucuronidase, have been found to progressively increase. Dipeptidase and tripeptidase were not found to …
-
Η ΣΗΜΑΣΙΑ ΤΟΥ ΠΡΟΣΔΙΟΡΙΣΜΟΥ ΩΡΙΣΜΕΝΩΝ ΒΙΟΛΟΓΙΚΩΝ ΠΑΡΑΜΕΤΡΩΝ ΣΤΗ ΜΕΛΕΤΗ ΤΟΥ ΚΑΡΚΙΝΟΥ
… phosphohexosoisomerase, gamma-glutamyl transpeptidase, 5'-nucleotidase, and carcinoembryonic antigen were determined in 256 cancer patients in order to evaluate their significance in diagnosis and patient monitoring. Our results indicate that in most cases there is a marked increase of …
-
The penicillin-binding proteins of Bacilli
… by benzylpenicillin of the natural peptidoglycan transpeptidase reaction that is catalysed by cell-wall/ membrane preparations of this organism. PBP1 is proposed to be the peptidoglycan transpeptidase that represents the lethal target of penicillin action. Penicillin-binding proteins have been …
-
Molecular Basis of Corynebacterium Diphtheriae Virulence and Infection In The Caenorhabditis Elegans Model Host
… onto the bacterial peptidoglycan by a specific transpeptidase enzyme called sortase. Although the SpaA pili are shown to be specific for pharyngeal cells in vitro, little is known about functions of the three pili in bacterial pathogenesis. This is mainly due to lack of in vivo models of …
-
Isolierte Gehirn-Kapillaren als in vitro-Modell der Blut-Hirn Schranke
… erhöhte Aktivitäten der Markerenzyme g-Glutamyl-Transpeptidase und Alkalische Phosphatase. Durch immunhistochemische Färbungen, Western Blots sowie Sekretions-Untersuchungen am konfokalen Lasermikroskop mit spezifischen Substraten und Hemmstoffen konnte belegt werden, daß sowohl P-Glykoprotein …
-
Association Between Metabolic Markers and Amount of Alcohol per Occasion in Korean Men
… alanine aminotransferase(ALT), r-glutamyl transpeptidase(r-GT) and uric acid. SUBJECT: A total 2,996 healthy men above 20 years of age who had visited a health promotion center in a university hospital from January, 2006 to December, 2006. METHOD: Subjects were classed as nondrinker, or …
-
Identifying Sortase A Variants With Higher Catalytic Effeciency
… A (SrtAstaph), a membrane-anchored cysteine transpeptidase present in gram-positive bacteria, covalently anchors virulence-associated surface proteins to the peptidoglycan cross bridge of the cell wall. SrtAstaph, one of the most characterized sortases, has found numerous applications in the …
-
SORTASE AND CHEMICAL BIOLOGY AS TOOLS TO STUDY UBIQUITINATION
… we leverage the power of sortase, a bacterial transpeptidase enzyme crucial to cell wall synthesis, to develop new strategies for studying protein ubiquitination. As one new technology, we introduce point mutations to the ubiquitin sequence in combination with click chemistry and genetic code …
-
Assembly and Display of Surface Proteins In Actinomyces Oris
… their covalent attachment to peptidoglycan by transpeptidase enzymes called sortases. Furthermore, a subset of cell wall-anchored proteins are polymerized to form pili prior to being anchored. I found that pilin proteins require a distinct signal peptidase for their maturation and function and …
-
Effects of Protein Domains on Localization of Penicillin-Binding Proteins 2a and 2b in Bacillus Subtilis
… are class B PBPs which have been known to have transpeptidase activities and they localize at different positions on the cell membrane. PBP2a spreads around the cylindrical wall as well as some at the septum, and PPB2b localizes exclusively to the septum and some at the cell poles. Both PBP2a …
-
Studies of tumor promoters and drug-metabolic enzymes in hamster buccal pouch mucosa
… initiating effects in the model. Gamma-glutamyl transpeptidase (GGT) and placentalglutathione S-transferase (GST-P) have been found to be increased during HBPM carcinogenesis. Whether such increases are oncofetal remains to be answered. There are few studies on the normal distribution of GGT and …
-
Studies of the Class A High-Molecular Weight Penicillin-Binding Proteins in Bacillus subtilis
… performing the penicillin-sensitive transpeptidase activity and the glycosyl transferase (GT) activity required for the polymerization of the glycan strands. The Class A PBPs in Bacillus subtilis are PBP1, PBP4, PBP2c, and PBP2d (YwheE) and they are encoded by ponA, pbpD, pbpF, and …
-
Development and Application of Mass Spectrometry Based Methodologies to Study Ubiquitin-Like Modifiers
… specificity of the bacterial protease- transpeptidase Sortase A (SrtA). Using a mutant with increased catalytic activity, SrtA mediates biotinylation of diglycine tryptic remnants, and also acts as a specific protease for release of isopeptides from streptavidin for analysis by mass …
-
Novel extracellular products of Mycobacterium tuberculosis: composition, synthesis, and relevance to disease
… was performed. Similar to other γ-glutamyltranspeptidases (GGTs), Rv2394 had a conserved catalytic motif consisting of a Threonine (Thr) residue. Mutating this Thr residue to Alanine (Ala) abrogated the enzymatic activity of Rv2394, including its autocatalytic activation. In contrast to …
-
Untersuchungen zur Induktion und zum Transfer der Vancomycin-Resistenz vom VanA-Typ sowie zur Flavophospholipol-Resistenz in Enterococcus faecium
… mit Transglycosylase- und Transpeptidase-Untereinheit handeln muss.
-
Sortases: Keystones to Virulence and Targets for Anti-Infective Therapy
… class of enzymes. Sortases are extracellular transpeptidases unique to Gram-positive bacteria. Their function is to covalently attach secreted virulence factors to the bacterial cell wall. Deletion or inhibition of sortases results in severe attenuation of bacteria for infection. In order to …
-
Development of Bacillus subtilis spores and cells for surface display of proteins
… of cell-based cellulase chip when using the SrtA transpeptidase. In contrast, CelA was verified to be successfully displayed on the spore surface using CotB and CotG, but not CotC, as carriers. In general, a large volume of culture (up to one liter) must be prepared containing both cells and …
-
Rat Lymphocyte Surface Glycoproteins Mediating Mitogenesis by Periodate or Neuraminidase Plus Galactose Oxidase
… membrane markers: 5'-nucleotidase, γ-glutamyl transpeptidase, and alkaline phosphodiesterase. The labeling was shown to be specific and uniform for all oxidized components; radioactive Schiff base adducts could not be detected following <sup>3</sup>H-borohydride reduction. Similar sets of …
-
An exploratory study of Helicobacter suis control strategies
… (NapA), the apoptosis-inducing γ-glutamyl transpeptidase (GGT), and some type IV secretion systems were also identified in H. suis. H. suis also possesses several presumptive virulence-associated genes, including homologs for mviN, the H. pylori flavodoxin gene (fldA) and the H. pylori …