Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"transmembrane helix"”.
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Defining Topologically Oriented Transmembrane Helix Contacts in Integral Membrane Protein EmrE by a Modified Two-Hybrid Approach
… throughput assay, constructs of individual EmrE transmembrane helices (TMH) were utilized to perform contact mapping of EmrE dimer structure. The results were compiled to construct a transmembrane helix interaction structure. This structure obtained is novel and identifies inconsistencies from …
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Topological Regulation of TM4SF20
Transmembrane proteins must adopt proper topology to perform their functions. It was previously demonstrated that ceramide regulates TM4SF20 (transmembrane 4 L6 family 20) by altering the topology of the transmembrane protein, but the underlying mechanism remains obscure. This regulatory mechanism, …
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The contribution of His7.36(305) of the GnRH receptor to ligand binding and receptor activation
… in receptor function. A His7.36(3o5) residue on transmembrane helix seven of the GnRH receptor was investigated for its contribution to the overall function of the receptor.
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Functional and Structural Studies of the Human Voltage-Gated Proton Channel
… 1950s. These channels all share a conserved six-transmembrane helix topology (S1-S6) in which the first four transmembrane helices (S1-S4) form the regulatory voltage-sensor domain and the last two transmembrane helices (S5 and S6) comprise the ion-conducting pore domain. It was thought that all …
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NMR investigations of blood coagulation: Conformational changes of membrane bilayers and proteins in blood coagulation
… of tissue factor (absent the membrane binding transmembrane helix) are assigned in solution and nanocrystalline samples by solution and solid-state NMR respectively. The chemical shifts are then used to investigate changes in the extracellular domain of tissue factor when bound to the membrane …
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The dual-targeted membrane assembly of the <i>Streptomyces coelicolor</i> Rieske protein
… proteins. The Sec machinery inserts multiple transmembrane domains into the cytoplasmic membrane by a co-translational mechanism, whereas, the Tat machinery translocates fully folded proteins across the membrane and is only known to integrate proteins that carry a single transmembrane …
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De Novo Heme Protein Design
… the 4--5 loop is as hydrophobic as the fourth helix of OR, charge neutralization of metal ion increases its hydrophobicity, thus it might turn into transmembrane helix and replace the fourth helix. Endogenous ligand binding to the metal site might disturb the charge balance and triggers helix …
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Mapping protein-protein interactions in the <i>Escherichia coli</i> Twin Arginine Translocase
… identified a binding site for TatA at the sixth transmembrane helix of TatC occupied in the resting state, indicating that TatA interacts constitutively with the TatBC recognition complex. Further crosslinking experiments found that the protonmotive force was required for TatA to occupy this …
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Molecular genetic manipulation and characterization of the high-potential chain of cyclic electron transport in Rhodobacter sphaeroides
… to the membrane by a single amino-terminal, transmembrane helix. Sequence homology with dioxygenase subunits binding a spectrally similar 2FE-2S cluster, suggests the fully conserved residues of the cytochrome bc$\sb{1}$ and b$\sb{6}$f sequences ligating the iron-sulfur cluster may be the …
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Novel Strategies towards the Inhibition of ATP-Binding Cassette (ABC) Transporters from Pathogenic Bacteria
… or that target hydrophobic pockets in the transmembrane domain. While some promising steps have been made, studies of inhibition of bacterial transporters still need to catch up to their eukaryotic counterparts. This work aimed to elucidate the characteristics of inhibition of MsbA and …
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Resolving Signal Transduction in Complex Biological Environments
… and G-protein coupled receptors (GPCRs), transmembrane signaling in enzyme-linked receptors have so far remained elusive due to the presence of only a single transmembrane helix in their structure for signal propagation. In this work, we explore the signal transduction in the epidermal …
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Effects of 2ʹ-fucosyllactose on brain and cognitive development of intrauterine growth restricted piglets
… to integral components of membranes and transmembrane helix, while down-regulated genes were clustered based on ion channel and transport, zinc ion binding, zinc-finger, and metal-binding. Finally, in the size by diet interaction, there were no clusters discovered as a result of …
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Towards atomistic characterization of blood coagulation proteins by NMR spectroscopy
… 15N mTF samples, a construct that includes the transmembrane helix, have been incorporated into both phospholipid Nanodiscs and POPC/DPPC liposomes. The impact of different lipid preparations on the structure of TF as well as mTF functional assay data are presented. Our preliminary results …
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The Heme -Copper Oxidoreductase Superfamily: Genomics and Structural Analyses
… is found to originate within a different transmembrane helix than in the A- and B-families. We then use mass spectrometry to verify that the predicted histidine-tyrosine crosslinked cofactor is formed in the active site of the C-type oxygen reductases. This is the first known example of …
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A Simulation Approach to the Study of Bacterial Secretion Proteins
… of the major pseudopilin PulG containing one transmembrane helix were simulated, demonstrating N-terminal interactions made possible by wild-type methylation of residue Phe1. Simulations of several monomeric PulG variants provided insight into the roles of the essential residue Glu5 and Phe1 …
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Structure and Function of the ER-Membrane Complex
… and in defining the topology of the first transmembrane helix in GPCRs. In this thesis I investigate the role of the EMC in the biogenesis of misfolded ABC transporters Yor1$\Delta$FRT and the homologous CFTR$\Delta$F. I expand on previous work on Yor1$\Delta$FRT to reveal that loss of EMC …
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Structure, Function and Dynamics of G-Protein coupled Receptors
… to elucidate the molecular mechanisms by which transmembrane signaling based physiological processes,i. e., the interactions of extracellular ligands with membrane-bound receptors, are regulated. In this work, synthetic transmembrane segments derived from the visual photoreceptor rhodopsin, the …
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I) Hv1, LBP and LCF reconstitution in Liposomes II) Signed, Sealed, and Delivered: CPP Technology Delivers Active Protein Cargo
… the conformational change within the 4th transmembrane helix of the Hv1 protein. Active Hv1 was achieved with two separate protein preparations as shown by quenching of the fluorescent dye ACMA after a valinomycin-induced membrane potential. Technical difficulties prevented further …
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Probing the organisation of the TatC component in the Tat system of <i>Escherichia coli</i>
… a substrate-induced interaction at the fifth transmembrane helix of TatC. Fluorescence microscopy experiments revealed that the dominant negative TatC variants prevented the polymerisation of TatA-YFP in vivo. These results show that TatC possesses at least two interaction interfaces and imply …
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Dynamics of protein-membrane interactions in drug metabolism and cancer
… of P450 enzymes provide insight into how transmembrane helix mutations might modulate effector interactions, and a molecular dynamics study of homotropic cooperativity brings atomic-scale mechanistic insight to P450 drug metabolism. Orientation measurements of KRas4b will also be described …
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