Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 130 for “"transmembrane domain"”.
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Biochemical Analysis of SNARE Protein Interactions – Role of Transmembrane Domain
SNARE (soluble NSF attachment protein receptor) Proteine umfassen verschiedene Familien von Proteinen, welche in Eukaryonten von der Hefe bis hin zum Menschen vorkommen. Sie sind für die Membranfusion bei zellulären Prozessen, wie Vesikeltransport, Neurotransmitterausschüttung oder …
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Evaluating the Interaction Between the Human Melanocortin-2 Receptor and the Accessory Protein, Mrap1: Chimeric Receptor and Alanine Substitution Studies on Transmembrane Domain 4, Extracellular Loop 2, and Transmembrane Domain 5
… melanocortin hormone, ACTH. MRAP1 is a single transmembrane-spanning domain protein that creates a homodimer with another MRAP1 protein. Furthermore, MRAP2 creates a heterodimer with the MC2R. Previous studies have shown that the MRAP1 protein contains an activation motif required for …
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Mutational analysis of the Vam3 transmembrane domain and its effects on vacuole fusion in Saccharomyces cerevisiae
… The specific role of the Vam3 trans-membrane domain (TMD) during vacuole fusion remains unclear. Previous work had shown that replacing Vam3 TMD with a prenyl lipid anchor (CCIIM) results in attenuated fusion and accumulation of trans-SNARE complexes, indicating CCIIM can not transit enough …
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The Role of Transmembrane Domain Helix-Helix Interactions in the Function of Pentameric Ligand-Gated Ion Channels
… anionic and neutral lipids. The lipid-exposed transmembrane alpha-helix, M4, in each homologous subunit likely acts as a lipid sensor. One model proposes that activating lipids promote M4 “binding” to the adjacent alpha-helices, M1 and M3, to enhance interactions between the M4 C-terminus and …
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A signal for retention of cytochrome P450 2C1 in the endoplasmic reticulum
… retention of proteins with a large cytoplasmic domain is the default pathway, the cellular distributions of a series of P450 2C1 and EGFR chimeric proteins were examined. The data indicate that the cytoplasmic domain of the protein also has retention properties. Protein containing only the …
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Calcium Channel Gamma(6) Subunit: In Search of Its Functions and Mechanisms of Action
… studies from our lab indicated that the first transmembrane domain of gamma6 is responsible for its inhibitory function. Using mutational analysis, we further identified the first GxxxA motif in the first transmembrane domain of gamma6 as being essential for the interaction between gamma6 and …
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Investigation of Prestin Membrane Topology by Substituted Cysteine Accessibility Method.
… two contradicting topology models: the Dallos 12 transmembrane domain model, and the Santos-Sacchi 10 transmembrane domain model. These two models are grossly different, in that the extracellular loops in one model are intracellular loops in the other. I hypothesize that prestin topology is a …
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Vývoj vybraných CD znaků a jejich role ve fylogenezi imunitního systému člověka
… been used. The intent was to identify at the domain level the first organism in which it is possible to find the searched molecule. At the N-terminal domain of the CD38 of birds, a sequence showing significant similarity to the archaebacterial flagellin domain was found. This flagellin …
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An Investigation of the Phospholamban-Serca Regulatory Interaction with Fluorescence Resonance Energy Transfer
… an alternative binding site. We propose that the transmembrane domain of PLB could move independently of its cytosolic domain. Our fluorescent probe is attached to the cytosolic domain of PLB, which would explain our inability to detect a change in the position of the transmembrane domain. My …
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Ceramide-Induced Alternative Translocation of TM4SF20
The polytopic membrane protein TM4SF20 (transmembrane 4 L6 family 20) is a protein containing four transmembrane helices that inhibits the Regulated Intramembrane Proteolysis (RIP) of the transcriptional factor CREB3L1 (cAMP response element binding protein 3-like 1), a transcription factor …
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The Translocase of the Outer Membrane of Mitochondria (TOM complex)
… elements were identified in this region: (i) a transmembrane domain (amino acid residues 45-68), (ii) a presequence-like helix (residues 69-83), and (iii) an import-auxiliary sequence (residues 84-126). The contribution of each of these elements to import was studied. The transmembrane domain …
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PhoQ: Structural and Mechanistic Investigations into an Important Bacterial Sensor Kinase
… other pathogenic gram negative bacteria. Certain domains of PhoQ and other two-component systems have been studied extensively and this research has yielded several structures with atomic level resolution. However, no complete structure or experimentally-based model has been forthcoming and the …
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Characterization of structure and dynamics of membrane proteins from solid-state NMR
… membrane proteins: 1) the N-terminal ectodomain and C-terminal cytoplasmic domain of influenza A virus M2 and 2) HIV-1 glycoprotein gp4l membrane-proximal external region and transmembrane domain (MPER-TMD) in a near native membrane environment. The cytoplasmic domain of M2 is necessary …
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Site-directed mutagenesis and molecular modeling studies of h5-HT7(a) receptor reveal important residues for binding and activation
… residues have been identified in the 7th transmembrane domain and in the second intracellular loop of the h5-HT7(a) receptor.
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Functional studies on receptor-type protein tyrosine phosphatases of the R3 subgroup
… They are characterised by an extracellular domain (ECD), comprised of multiple FNIII-like repeats, a transmembrane domain and a single intracellular phosphatase domain. Although their phosphatase domains have been Fstudied in detail the functional roles of their extracellular regions have …
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The role of tapasin and its isoforms in antigen presentation and tumor immunity
… by confocal microscopy. Isoform 3 lacks a transmembrane domain and was found to be secreted from cells as well as being found within the ER. All isoforms were widely expressed at the RNA level in many tissues and cell types; however, mature dendritic cells (DCs) expressed the highest levels …
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Identification and characterization of a novel structural protein of porcine reproductive and respiratory syndrome virus, the replicase nonstructural protein 2
… isolates.</p> <p>Next the hydrophobic domain of nsp2 was characterized as a putative multi-pass transmembrane domain predicted to facilitate nsp2 packaging through association with the viral envelope. Within an in vitro cell-free translation system nsp2 was found to strongly associate …
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Differential glycosylation of Tractin and LeechCAM, two novel Ig-superfamily members, regulates neurite extension and fascicle formation
… unusual structure: it contains six Ig-like domains, four FNIII-like domains, an acidic-domain, twelve repeats of a novel proline-and glycine-rich motif, a transmembrane domain, and an intracellular domain with an ankyrin binding motif. Tractin possesses a potential cleavage site and evidence …
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Structure and dynamics of influenza M2 proton channels from solid-state NMR
… proton channel activity is encapsulated by a transmembrane domain (TM) that is targeted by the FDA-approved antiviral drugs amantadine and rimantadine to inhibit viral replication. Proton conduction by M2's TM is mediated by a His-xxx-Trp motif conserved in the otherwise disparate M2 from …
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Understanding Host Antiviral Signaling Pathways
… NF-kappa B and IRFs through its N-terminal CARD domains. An important area of research is to identify the downstream host factors that participate in these pathways and to determine the mechanism by which they function. Here, I describe the development of an in vitro biochemical assay to detect …
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