Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"translocation mechanism"”.
-
Artificial Transmembrane Signalling
… an indirect artificial transmembrane signalling mechanism which operates by controlled translocation of a synthetic transducer across the lipid bilayer was investigated. The fundamental concept of the translocation mechanism is the switching of membrane-permeability of the two head groups of the …
-
Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
… and optical tweezers force spectroscopy to study translocation and unwinding mechanism of Escherichia coli Rep, a model helicase with 3’5’ motor translocation activity on single‐stranded DNA (ssDNA) fueled by ATP hydrolysis. When a translocating Rep on a 3’‐overhang DNA reaches the DNA duplex …
-
Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
… and optical tweezers force spectroscopy to study translocation and unwinding mechanism of Escherichia coli Rep, a model helicase with 3’5’ motor translocation activity on single‐stranded DNA (ssDNA) fueled by ATP hydrolysis. When a translocating Rep on a 3’‐overhang DNA reaches the DNA duplex …
-
Ehrlichia chaffeensis TRP32 is a Nucleomodulin that Regulates Host Gene Expression and Post-Translational Modifications Determine its Localization and Function
… enters the host cell nucleus via a noncanonical translocation mechanism that involves phosphorylation of Y179 located in a C-terminal tri-tyrosine motif. Both genistein and mutation of Y179 inhibited TRP32 nuclear entry. I also show that TRP32 is mono and poly- ubiquitinated on multiple lysine …
-
Super-Resolution Force Spectroscopy for Revealing Sub-nucleotide Motion in Ribosomal Frameshifting and DNA Methylation
… transfer (FRET) have been utilized to study the translocation mechanism. However, it remains challenging to directly resolve the different reading frames. This dissertation focuses on applying super-resolution force spectroscopy (SURFS) as a unique tool to resolve frameshifting products and …
-
Delivery of biomolecules into mammalian cells using anthrax toxin
… and functionalities. Collectively, these translocation studies have helped to elucidate the translocation mechanism and to probe intracellular biological processes. In this thesis, the PA/LFN delivery platform was used to analyze the delivery of assorted biomolecules through the PA pore. A …
-
Structural Studies of Protein Transport
… core, which plays a similar role in a similar translocation mechanism and uses a similar set of amino acids. This indicates that proto-SecY evolved its translocation pore by bringing together the grooves of two Oxa1 proteins. This model provides a new paradigm for understanding how these …
-
The mechanisms of aquaporin expression and translocation in cerebral pathologies
… this thesis primarily elucidates the regulatory mechanisms involved in processes of hypotonicity and hypoxia mediated AQP4 translocation in primary astrocytes and examines current therapies for cerebral oedema including hypothermia. Using confocal microscopy, cell surface biotinylation, mutants …
-
A study of protein-DNA interactions using atomic force microscopy and DNA origami
… regulatory proteins. Better understanding of the mechanisms used by these natural proteins should facilitate the use of genome editing tools. In nature, gene regulation is usually sparked by a change in the cellular environment, such as viruses invading a bacterium. Restriction enzymes defend the …