Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 36 for “"translocase"”.
-
The Translocase of the Outer Membrane of Mitochondria (TOM complex)
… with N-terminal targeting signals. The TOM translocase was found to bind precursor proteins efficiently in a specific manner in the absence of chaperones and lipids in a bilayer structure. Following the initial binding, the presequence was transferred into the translocation pore in a step …
-
THE BIPC MINOR TRANSLOCASE MEMBRANE PROTEIN OF THE BURKHOLDERIA TRANSLOCON
… up the tip of the needle, BipB is the major translocase, and BipC is the minor translocase. Major and minor translocator proteins form a pore for bacterial effector transfer. BipC is predicted to be the minor translocase in Burkholderia pseudomallei. It is postulated that BipC, minor …
-
Mapping protein-protein interactions in the <i>Escherichia coli</i> Twin Arginine Translocase
The Twin Arginine Translocase (Tat) system is a membrane-bound transport system present in plants and bacteria that has the remarkable ability to export fully folded proteins across a lipid bilayer, powered by the protonmotive force. In Escherichia coli the Tat system is composed of only three …
-
Molekulare Ursachen des Mohr-Tranebjaerg-Syndromes
… a defects in the biogenesis of the human TIM23 translocase Tim8 and Tim13 of yeast belong to a family of evolutionary conserved zinc finger proteins that are organised in hetero-oligomeric complexes in the mitochondrial intermembrane space (IMS). The TIM8-13 complex assists the import of Tim23, …
-
Investigations into the roles of bacterial TorD family chaperone proteins
The twin-arginine translocase (Tat) is a highly specialised protein transport system, present in prokaryotes and plant chloroplasts. This translocase functions to transport proteins across the cytoplasmic membrane in a fully folded state. Substrates of the Tat system are targeted to the system by …
-
Single Molecule Förster Resonance Energy Transfer and Super Resolution Force Spectroscopy Study of Elongation Factor G Evolution, Conformation, and Function
… the ribosome decodes mRNA facilitated by a translocase enzyme, elongation factor G (EF-G). EF-G induces the advancement of the mRNA reading frame by 3 nucleotides (nt) and tRNA translocation via the hydrolysis of one GTP molecule. The mechanism of this reaction remains largely unknown. …
-
A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
… phospholipids, and the membrane-embedded translocase SecYEG. Functional studies implied that some of these interactions occurred simultaneously. However, little is known about the details of these binding sites, and it was not clear to what extent SecA interacted with these diverse ligands …
-
Mechanistic studies of a AAA+ protease
… of the hexameric ClpX unfoldase and polypeptide translocase and the ClpP compartmental peptidase. ClpX binds a substrate by an unstructured degradation tag and then, by multiple rounds of ATP-binding and hydrolysis, unfolds and translocates the substrate into the proteolytic chamber of ClpP. To …
-
Discovery of Natural Product-based Antimycobacterial Agents Effective against Non-replicating Bacilli
… in their enzymatic inhibitory activities against translocase I (MraY/MurX) and prenyl-phosphate-GlcNAc-1-phosphate transferase (WecA). UT-01320 (45) is identified as a selective WecA inhibitor that kills both replicating and non-replicating Mtb at 1.50 μg/mL (MIC) and 2.58 μg/mL, respectively. …
-
THE ROLE OF SKELETAL MUSCLE-SYNTHESIZED BRAIN DERIVED NEUROTROPHIC FACTOR IN THE MAINTENANCE OF MOTOR NEURON MITOCHONDRIAL POPULATIONS
… labeling to target the mitochondrial translocase of outer membrane, TOM20. We used immunolabeling to further delineate mitochondrial populations within gastroc-associated motorneurons. To determine if experimental groups exhibit altered mitochondrial populations, we used confocal …
-
XPD Helicase: Shifting the Inchworm Into Reverse
… This work characterizes XPD as a 5' to 3' ssDNA translocase that preferentially unwinds forked DNA substrates, contains an FeS cluster, binds to the ssDNA-dsDNA junction of forked substrates, and is capable of unwinding forked substrates up to 40 bps as a monomer in the presence of RPA2. I found …
-
Functional Analysis of FtsA-Mediated Recruitment of FtsK in Escherichia coli
… septal synthesis and recruiting the DNA translocase FtsK was examined using mutational analysis in <em>Escherichia coli</em>.</p> <p>The FtsA<sup>M96E R153D </sup>variant, which has previously been shown to disrupt double-stranded (DS) filament formation, was unable to support cell …
-
AN INSIGHT INTO DIFFERENT MODES OF REMODELER REGULATION: FOCUS ON SACCHAROMYCES CEREVISIAE SWI/SNF
… share a conserved ATP hydrolysis and DNA translocase domain, their biochemical actions and in-vivo characteristics differ because of their subunits and accessory domains in the catalytic subunit that regulate its activity. Understanding how these domains contribute to remodeler regulation …
-
DNA double-strand break repair studied by atomic force microscopy
… activity of the NurA nuclease and hexameric HerA-translocase to generate long single-stranded DNA overhangs essential for DSB repair by HR in archaea. The present data verify and clarify the presumed biological role of this complex. Overall, the present study provides new insights into the initial …
-
Fancm and Faap24 Maintain Genomic Stability Through Cooperative and Unique Functions
… bypass factors to ICL damage sites through its translocase activity. Collectively, my studies provide mechanistic insights into how genome integrity is both coordinately and independently protected by FANCM and FAAP24.</p>
-
Communication & coordination between components of the ClpAP degradation machine
… ClpA is a double-ring hexameric AAA+ unfoldase/translocase that functions with the tetradecameric ClpP peptidase to degrade proteins that are damaged, unneeded, or require degradation for regulation. ClpA has two distinct, stacked rings, termed D1 and D2, constructed from hexamerization of …
-
Protective antigen-mediated delivery of biomolecules
… Protective antigen, a naturally evolved protein translocase derived from Bacillus anthracis, has shown promise as a platform of protein delivery due to its ability to form a transmembrane pore that allows the cargo to have cytosolic access. We and others have used the LFN/PA system to deliver a …
-
Functions of Human DNA2 and its Protein Partners in DNA End Resection
… or BLM. We propose that the DNA2 motor acts as a translocase on single-stranded DNA accelerating the degradation by the nuclease domain of hDNA2. I also participated in several collaborative projects showing that DNA2 cooperates with WRN/BLM in DNA end resection and the restart of reversed …
-
!Development of small molecule therapeutics against anti-infectious and anti-cancer drug resistance via structure-based drug design
… (SARs).Phospho-N-acetyl-muramyl-pentapeptide translocase (MraY) is an important membrane enzyme involved in the early-stage biosynthesis of bacterial peptidoglycans. As the inhibition of MraY leads to bacterial cell lysis, such MraY inhibitors (e.g., muraymycin) hold great promise for …
Page 1 of 2