Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"translatome"”.
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Genetic regulation of maize floral development - insights from the transcriptome and translatome
… gene expression regulation at transcriptome and translatome level to gain insights into floral development. To transcriptionally gain insight into the functional differences between florets with different fates, I examined gene expression in upper and lower floral meristems in maize ear using …
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Computational and experimental detection of uncoupling between transcriptome and translatome changes of gene expression
… extensive uncoupling between transcriptome and translatome variations of mRNA levels emerges, measured by a significant difference between steady state and polysomal fold changes induced by a cellular physiological or pathological transition. It seems clear that virtually the majority of …
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A multiplexed approach for quantitative profiling of the translatome using bioorthogonal non-canonical amino acids
… synthesis rate at any given time (i.e the Translatome). At this time, measurements of protein synthesis rates are limited to methods that use mRNA abundance as a proxy; however, there are regulatory steps on the level of translation that can confound correlation between mRNA abundance and …
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A multi-level approach of gene expression data analysis to investigate translatome dynamics across multiple tissues, stages, and mouse models of SMA
… advantage of ribosome profiling to obtain the translatome from multiple tissues, stages and disease mouse models. Given that SMN is ubiquitously expressed, brain, spinal cord and liver were collected to investigate if common features underly translational defects upon its loss in these tissues. …
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Translational control mechanisms in the p53 response network
… gene expression responses, we performed translatome analyses upon its activation either by different agents or cellular contexts. Considered as a proxy for the proteome, the translatome allows us to characterize the translational status of each mRNA, independently from transcriptional …
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Mechanisms of selective translational control under short-term high light stress
… early layer in the journey to a tailored translatome upon high light stress. More specific regulatory processes evidently affect translation downstream of stress signaling, targeting differential mRNA characteristics, specific mRNA recognition via interaction with RNA-binding proteins …
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Host lncRNAs recognize invading bacteria and aid against infection by associating with polysomes
… monitored changes in the transcriptome and the translatome upon infection at early time-points after infection and studying the possible function and mechanism of two lncRNAs that I found to be over-expressed upon infection. RESULTS: I showed that infection with either bacterial strain induced …
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Network-based approaches for multi-omic data integration
… data such as genome, epigenome, transcriptome, translatome, proteome, metabolome and interactome. Biological systems are highly dynamic and complex mechanisms which involve not only the within-level functionality but also the between-level regulation. In order to uncover the complexity of …
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New Tools to Understand Mechanisms of Nutrient Transfer from Plants to Biotrophic Pathogens
… nature. I have developed a method to isolate the translatome (i.e., mRNAs associated with ribosomes) of pathogen-proximal cells. This method utilizes translating ribosome immuno-purification technology (TRAP), regulated by both pathogen-responsive and tissue-specific promoters, to isolate mRNAs …
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Translational mechanisms of stem cell fate regulation in epidermal oncogene tolerance
… of a substantial subset of the oncogenic RAS translatome. Furthermore, by coupling ribosome profiling with genetic screening, we provide direct functional evidence that RAS-induced differentiation is driven by eIF2B5-mediated translation of ubiquitination genes. We reveal ubiquitin ligase …
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Massive Exchange of mRNA between a Parasitic Plant and its Hosts
… of host mRNAs by detecting them in the Cuscuta translatome as revealed by sequencing polysomal RNA and ribosome-protected RNA. This work highlights RNA trafficking as a potentially important new form of interaction between hosts and Cuscuta.
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Overcoming radioresistance by targeting p21 in cancer
… loss of p21 induced substantial changes in the translatome, with reduced polysome-bound RNA, confirming altered gene expression post-radiation. In response to radiation, p21 recruits a multi-faced interaction network favoring survival after DNA-damage, cytoskeletal stability, vesicles …
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Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression
… to the swine industry worldwide. Here, the viral translatome was characterised using ribosome profiling (RiboSeq), a high-throughput sequencing-based technique that allows the positions of translating ribosomes to be mapped to a genome with sub-codon precision. This was used in parallel with RNA …
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Role of 4E-BP2 in neuronal translation
… in a few residues. Moreover, using unbiased translatome mapping, we discovered that overexpression of deamidated 4E-BP2 represses the translation of a distinct pool of mRNAs linked to cerebral development, mitochondria and chiefly NF- κB activity. Collectively, these data describe a …
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Integrative analysis of heterogeneous plant cell wall related data
… of the Arabidopsis root transcriptome and translatome for the identification of cell wall related genes and compare expression patterns of cell wall synthesis genes. The computational analysis considered correlation and variation of expression across cell-types at both system-levels, and …
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Commonalities between SMA and ALS: investigation of ribosome heterogeneity and translational defects
… with the translation machinery. In addition, translatome changes are commonly observed in both diseases and loss of SMN and dysregulation of TDP43 and MATR3 are associated with translation defects of specific mRNAs. Therefore, exploration of a direct involvement of these proteins in …