Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"translation inhibitor"”.
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Characterization of endocytic escape pathway and its role in adaptive immunity
… When saporin enters the cytosol, it causes translational arrest quantified using puromycin labelling of newly synthesized polypeptides. Using the saporin assay, the Kozik lab performed CRISPR/Cas9 genetic screen targeting 200 candidate genes. The screen yielded one strong hit: pore-forming …
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Identifying Novel Transcriptional Effectors of the Juvenile Hormone Pathway in Aedes aegypti
… reproductive tissues in the presence of a translation inhibitor, we identified two early JH responsive gene candidates, FoxA and zinc finger 519, p-value <0.05. The functional characterization of these two remains to be seen, however, in Drosophila melanogaster, they both have roles in …
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Semisynthetic Development of Blasticidin S as an Antibiotic Lead and Development of Other Anti-Infectives
… one such forgotten compound, the broad-spectrum, translation inhibitor, blasticidin S. We produced three series of semisynthetic derivatives of blasticidin S that show a marked increase in antibacterial activity against gram-positive bacteria and an increase in selectivity index for pathogenic …
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Investigating temperature signalling pathways in Arabidopsis thaliana using small molecules
… of plant temperature responses. In addition, the translation inhibitor cycloheximide was identified as a repressor of heat-inducible genes and an activator of early cold-inducible genes. Diverse translation inhibitors trigger a cytosolic influx of calcium ions and several inhibitors of translation …
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Energy Stress Causes Chaperones to Assemble Into Cytoplasmic Complexes
… proteins to be anchored into the membrane post-translationally, exposing hydrophobic residues prone to aggregation. By performing the glucose v deprivation in the presence of cycloheximide, a translation inhibitor, I observed that, indeed, newly synthesized proteins must be present for the …
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Characterisation of two aphid picorna-like viruses
… were identified by immunoprecipitation. ALPV translation products were post-translationally cleaved as demonstrated in pulse-chase experiments and in experiments using a translation inhibitor. The efficiency of cleavage was concentration-dependent indicating the action of a protease. In …
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Biochemical purification and functional characterization of the She RNP complex from S. cerevisiae
… proteins, including the kinase Gin4p and the translation inhibitor Eap1p. Eap1p has been of outstanding interest since a systematic RNA localization assay with ash1 mutants that contained premature stop codons inserted at various positions within the coding sequence have revealed severe …
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Mechanism-Based Strategies to Enhance The Actions of A
… I hypothesized that inhibition of transcription/translation of HSP or client proteins would enhance 17-AAG-mediated cytotoxicity. Multiple myeloma (MM) cell lines MM.1S, RPMI-8226, and U266 were used as a model. To test this hypothesis, two different strategies were used. For the first approach, …